Experiment set53IT054 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 1.98 mM 48 hr

Group: stress
Media: BHIS + Methotrexate (1.98 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 7 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter oligopeptide (TC 3.A.1.5.1) 5
Nitrate and nitrite ammonification 1
Sex pheromones in Enterococcus faecalis and other Firmicutes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.