Experiment set53IT052 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.06 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b1709 btuD +1.2 2.3 vitamin B12-transporter ATPase (NCBI) compare
b2741 rpoS +1.2 9.3 RNA polymerase sigma factor (NCBI) compare
b3341 rpsG +1.1 2.0 30S ribosomal protein S7 (NCBI) compare
b3206 npr +1.0 5.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1085 b1085 +1.0 3.3 orf, hypothetical protein (VIMSS) compare
b1150 ymfR +1.0 2.0 e14 prophage; predicted protein (NCBI) compare
b1823 cspC +0.9 3.2 stress protein, member of the CspA-family (NCBI) compare
b2829 ptsP +0.9 8.4 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2217 rcsB +0.9 6.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1954 dsrA +0.8 3.3 regulatory antisense RNA (NCBI) compare
b1628 rsxB +0.8 3.5 electron transport complex protein RnfB (NCBI) compare
b1600 mdtJ +0.8 2.2 multidrug efflux system transporter (NCBI) compare
b2688 gshA +0.8 5.7 glutamate--cysteine ligase (NCBI) compare
b1726 yniB +0.8 2.6 predicted inner membrane protein (NCBI) compare
b0082 mraW +0.8 4.5 S-adenosyl-methyltransferase (NCBI) compare
b0730 mngR +0.8 1.8 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3443 yrhA +0.8 2.0 orf, hypothetical protein (VIMSS) compare
b0955 ycbZ +0.8 5.8 predicted peptidase (NCBI) compare
b2659 b2659 +0.8 3.1 orf, hypothetical protein (VIMSS) compare
b1936 intG +0.7 1.0 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3753 rbsR +0.7 6.6 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b4505 insX +0.7 1.9 predicted IS protein (RefSeq) compare
b4676 yoaK +0.6 0.8 expressed protein (RefSeq) compare
b4133 cadC +0.6 5.8 DNA-binding transcriptional activator (NCBI) compare
b4590 ybfK +0.6 1.0 hypothetical protein (RefSeq) compare
b4215 ytfI +0.6 1.5 hypothetical protein (NCBI) compare
b0661 miaB +0.6 5.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b2947 gshB +0.6 2.7 glutathione synthetase (NCBI) compare
b1824 yobF +0.6 3.3 hypothetical protein (NCBI) compare
b0240 crl +0.6 4.6 DNA-binding transcriptional regulator (NCBI) compare
b1380 ldhA +0.6 2.6 D-lactate dehydrogenase (NCBI) compare
b1295 ymjA +0.5 2.0 hypothetical protein (NCBI) compare
b1613 manA +0.5 1.0 mannose-6-phosphate isomerase (VIMSS) compare
b1179 ycgL +0.5 2.0 conserved protein (NCBI) compare
b3181 greA +0.5 3.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1335 ogt +0.5 2.4 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b1765 ydjA +0.5 0.8 predicted oxidoreductase (NCBI) compare
b3146 yraL +0.5 3.5 predicted methyltransferase (NCBI) compare
b1849 purT +0.5 3.5 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1573 ydfC +0.5 1.4 Qin prophage; predicted protein (RefSeq) compare
b0388 aroL +0.5 3.6 shikimate kinase II (NCBI) compare
b3261 fis +0.5 1.3 DNA-binding protein Fis (NCBI) compare
b3816 corA +0.5 4.0 magnesium/nickel/cobalt transporter (NCBI) compare
b1642 slyA +0.5 2.0 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1627 rsxA +0.5 2.7 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b4557 yidD +0.5 2.6 hypothetical protein (NCBI) compare
b2903 gcvP +0.5 4.1 glycine dehydrogenase (NCBI) compare
b2528 iscA +0.5 1.2 iron-sulfur cluster assembly protein (NCBI) compare
b3766 ilvL +0.5 2.1 ilvG operon leader peptide (NCBI) compare
b2904 gcvH +0.5 1.8 glycine cleavage system protein H (NCBI) compare
b2136 yohD +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b0965 b0965 +0.5 1.1 orf, hypothetical protein (VIMSS) compare
b1346 ydaQ +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b4227 ytfQ +0.4 2.3 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b0864 artP +0.4 2.2 arginine transporter subunit (NCBI) compare
b1891 flhC +0.4 2.3 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b3929 rraA +0.4 3.3 ribonuclease activity regulator protein RraA (NCBI) compare
b2007 yeeX +0.4 0.8 conserved protein (RefSeq) compare
b0303 ykgI +0.4 0.5 orf, hypothetical protein (VIMSS) compare
b0382 yaiB +0.4 3.2 hypothetical protein (NCBI) compare
b2258 b2258 +0.4 1.0 putative transport/receptor protein (VIMSS) compare
b3523 yhjE +0.4 3.6 predicted transporter (NCBI) compare
b2061 wzb +0.4 1.6 protein-tyrosine phosphatase (NCBI) compare
b1492 gadC +0.4 2.6 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1839 yebY +0.4 1.9 hypothetical protein (NCBI) compare
b0535 fimZ +0.4 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4071 nrfB +0.4 1.8 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1233 ychJ +0.4 2.2 hypothetical protein (NCBI) compare
b4450 arcZ +0.4 2.1 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0696 kdpC +0.4 1.2 potassium-transporting ATPase subunit C (NCBI) compare
b2212 alkB +0.4 2.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b3516 gadX +0.4 3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2684 mprA +0.4 1.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1259 yciG +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1630 rsxD +0.4 2.0 electron transport complex protein RnfD (NCBI) compare
b1505 ydeT +0.4 0.9 hypothetical protein (RefSeq) compare
b1324 tpx +0.4 2.3 thiol peroxidase (NCBI) compare
b1246 oppD +0.4 2.9 oligopeptide transporter ATP-binding component (NCBI) compare
b1582 ynfA +0.4 1.4 hypothetical protein (NCBI) compare
b2929 yggD +0.4 2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3166 truB +0.4 2.5 tRNA pseudouridine synthase B (NCBI) compare
b1591 b1591 +0.4 0.9 putative oxidoreductase component (VIMSS) compare
b1194 ycgR +0.4 2.1 protein involved in flagellar function (NCBI) compare
b1754 b1754 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1013 ycdC +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3512 GadE +0.4 1.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0263 afuB +0.4 0.8 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b3858 yihD +0.4 1.7 hypothetical protein (NCBI) compare
b4391 yjjK +0.4 2.8 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2490 b2490 +0.4 1.4 putative protein processing element (VIMSS) compare
b1882 cheY +0.4 0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1400 paaY +0.3 1.0 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1306 pspC +0.3 1.7 DNA-binding transcriptional activator (NCBI) compare
b0998 torD +0.3 0.8 chaperone protein TorD (NCBI) compare
b4595 yciY +0.3 1.4 hypothetical protein (RefSeq) compare
b3027 ygiZ +0.3 0.3 conserved inner membrane protein (NCBI) compare
b3198 kdsC +0.3 1.3 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b1562 hokD +0.3 1.5 Qin prophage; small toxic polypeptide (NCBI) compare
b3293 yhdN +0.3 1.8 hypothetical protein (NCBI) compare
b1743 spy +0.3 1.6 envelope stress induced periplasmic protein (NCBI) compare
b4094 phnN +0.3 1.8 ribose 1,5-bisphosphokinase (NCBI) compare
b1603 pntA +0.3 1.8 NAD(P) transhydrogenase subunit alpha (NCBI) compare
b2570 rseC +0.3 2.3 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0468 ybaN +0.3 2.1 conserved inner membrane protein (NCBI) compare
b2650 ypjC +0.3 0.2 hypothetical protein (RefSeq) compare
b3366 nirD +0.3 1.9 nitrite reductase small subunit (NCBI) compare
b1407 ydbD +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b3468 yhhN +0.3 3.0 conserved inner membrane protein (NCBI) compare
b4653 yicT +0.3 0.7 no description compare
b3071 yqjI +0.3 1.7 predicted transcriptional regulator (NCBI) compare
b1760 ynjH +0.3 1.6 hypothetical protein (NCBI) compare
b2229 b2229 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4529 ydbJ +0.3 0.7 hypothetical protein (NCBI) compare
b2946 yggJ +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1190 dadX +0.3 2.0 alanine racemase (NCBI) compare
b1143 ymfI +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b4049 dusA +0.3 2.6 tRNA-dihydrouridine synthase A (RefSeq) compare
b1448 yncA +0.3 1.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2637 yfjT +0.3 1.1 CP4-57 prophage; predicted protein (NCBI) compare
b1138 ymfE +0.3 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b1278 pgpB +0.3 1.2 phosphatidylglycerophosphatase B (NCBI) compare
b1919 yedO +0.3 1.0 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1334 fnr +0.3 1.5 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0560 nohB +0.3 1.3 DLP12 prophage; DNA packaging protein (NCBI) compare
b1010 ycdK +0.3 1.3 hypothetical protein (NCBI) compare
b2181 yejG +0.3 1.8 hypothetical protein (NCBI) compare
b1632 rsxE +0.3 2.5 NADH-ubiquinone oxidoreductase (NCBI) compare
b1243 oppA +0.3 2.1 oligopeptide transporter subunit (NCBI) compare
b4642 yoeD +0.3 1.1 no description compare
b3153 yhbO +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b1396 paaI +0.3 1.8 predicted thioesterase (NCBI) compare
b4095 phnM +0.3 2.1 carbon-phosphorus lyase complex subunit (NCBI) compare
b1186 nhaB +0.3 2.5 NhaB sodium/proton transporter (Katherine Huang) compare
b1247 oppF +0.3 2.1 oligopeptide transporter subunit (NCBI) compare
b4614 sokA +0.3 1.0 no description compare
b0357 yaiN +0.3 0.9 putative alpha helix chain (VIMSS) compare
b4397 creA +0.3 1.8 hypothetical protein (NCBI) compare
b0191 yaeJ +0.3 1.7 hypothetical protein (NCBI) compare
b4203 rplI +0.3 1.6 50S ribosomal protein L9 (NCBI) compare
b0713 ybgL +0.3 2.2 hypothetical protein (NCBI) compare
b4585 sroB +0.3 0.6 Novel sRNA, function unknown (RefSeq) compare
b4235 pmbA +0.3 2.6 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b1482 osmC +0.3 1.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b4416 rybA +0.3 0.8 unknown RNA (NCBI) compare
b2450 yffS +0.3 1.1 CPZ-55 prophage; predicted protein (RefSeq) compare
b2445 yffN +0.3 1.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b0822 ybiV +0.3 1.5 predicted hydrolase (NCBI) compare
b3446 yrhB +0.3 1.6 hypothetical protein (NCBI) compare
b0065 yabI +0.3 1.6 conserved inner membrane protein (NCBI) compare
b2668 ygaP +0.3 1.9 predicted inner membrane protein with hydrolase activity (NCBI) compare
b2349 intS +0.3 2.0 CPS-53 (KpLE1) prophage; predicted prophage CPS-53 integrase (NCBI) compare
b1961 dcm +0.3 0.6 DNA cytosine methylase (NCBI) compare
b4594 ymgJ +0.3 1.1 hypothetical protein (RefSeq) compare
b1594 dgsA +0.3 1.3 DNA-binding transcriptional repressor (NCBI) compare
b4685 yrbN +0.3 1.1 expressed protein (RefSeq) compare
b3495 uspA +0.3 2.4 universal stress global response regulator (NCBI) compare
b3658 selC +0.3 1.3 tRNA-Sec (NCBI) compare
b4692 oweE +0.3 1.1 no description compare
b1244 oppB +0.3 1.8 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b0068 tbpA +0.3 1.9 thiamin transporter subunit (NCBI) compare
b0310 ykgH +0.3 0.6 predicted inner membrane protein (NCBI) compare
b4135 yjdC +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2660 ygaF +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b1245 oppC +0.3 1.8 oligopeptide transporter subunit (NCBI) compare
b1218 chaC +0.3 1.7 regulatory protein for cation transport (RefSeq) compare
b1382 ynbE +0.3 0.8 predicted lipoprotein (NCBI) compare
b2502 ppx +0.3 2.2 exopolyphosphatase (NCBI) compare
b1070 flgN +0.3 0.7 export chaperone for FlgK and FlgL (NCBI) compare
b2783 chpR +0.3 1.0 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b1125 potB +0.3 1.5 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b4695 ykgT +0.3 0.8 no description compare
b1139 lit +0.3 1.6 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b2474 ypfI +0.3 2.2 predicted hydrolase (NCBI) compare
b2471 yffB +0.3 1.4 hypothetical protein (NCBI) compare
b1394 paaG +0.3 1.1 enoyl-CoA hydratase (NCBI) compare
b3507 dctR +0.3 2.3 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b1640 ydhH +0.3 1.3 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1077 flgF +0.3 1.9 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b2448 yffQ +0.3 0.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b1890 motA +0.3 1.6 flagellar motor protein MotA (NCBI) compare
b1602 pntB +0.3 1.1 pyridine nucleotide transhydrogenase (NCBI) compare
b1900 araG +0.3 1.1 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0920 ycbC +0.3 1.3 conserved inner membrane protein (NCBI) compare
b0792 ybhR +0.3 1.6 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1949 fliQ +0.3 1.2 flagellar biosynthesis protein Q (NCBI) compare
b1696 ydiP +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4684 yqfG +0.3 1.8 expressed protein (RefSeq) compare
b1079 flgH +0.3 1.2 flagellar L-ring protein precursor H (NCBI) compare
b2346 vacJ +0.3 1.9 predicted lipoprotein (NCBI) compare
b1524 yneH +0.3 0.5 predicted glutaminase (NCBI) compare
b2377 yfdY +0.3 1.2 predicted inner membrane protein (NCBI) compare
b1164 ycgZ +0.3 0.7 hypothetical protein (NCBI) compare
b2811 ygdK +0.2 1.4 predicted Fe-S metabolism protein (NCBI) compare
b1487 ddpA +0.2 1.6 D-ala-D-a la transporter subunit (NCBI) compare
b1942 fliJ +0.2 0.5 flagellar biosynthesis chaperone (NCBI) compare
b1822 rrmA +0.2 1.2 23S rRNA m1G745 methyltransferase (NCBI) compare
b0616 citE +0.2 1.5 citrate lyase beta chain (acyl lyase subunit) (VIMSS) compare
b1302 puuE +0.2 1.4 GABA aminotransferase, PLP-dependent (NCBI) compare
b3130 yhaV +0.2 1.7 hypothetical protein (NCBI) compare
b3503 arsC +0.2 1.4 arsenate reductase (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms