Experiment set53IT049 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.06 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b1085 b1085 +1.1 4.8 orf, hypothetical protein (VIMSS) compare
b1765 ydjA +1.1 1.7 predicted oxidoreductase (NCBI) compare
b4603 rseX +1.0 1.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2741 rpoS +1.0 6.9 RNA polymerase sigma factor (NCBI) compare
b3206 npr +1.0 5.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2829 ptsP +0.9 8.5 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1954 dsrA +0.9 4.9 regulatory antisense RNA (NCBI) compare
b1150 ymfR +0.9 1.8 e14 prophage; predicted protein (NCBI) compare
b1642 slyA +0.9 4.2 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0955 ycbZ +0.8 6.8 predicted peptidase (NCBI) compare
b2007 yeeX +0.8 1.4 conserved protein (RefSeq) compare
b2168 fruK +0.8 1.3 1-phosphofructokinase (NCBI) compare
b1823 cspC +0.8 3.0 stress protein, member of the CspA-family (NCBI) compare
b2934 cmtB +0.8 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3753 rbsR +0.8 6.8 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2217 rcsB +0.8 5.8 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1824 yobF +0.7 4.3 hypothetical protein (NCBI) compare
b4585 sroB +0.7 1.8 Novel sRNA, function unknown (RefSeq) compare
b1628 rsxB +0.7 2.2 electron transport complex protein RnfB (NCBI) compare
b2688 gshA +0.7 4.8 glutamate--cysteine ligase (NCBI) compare
b3027 ygiZ +0.7 0.6 conserved inner membrane protein (NCBI) compare
b0082 mraW +0.6 3.3 S-adenosyl-methyltransferase (NCBI) compare
b4133 cadC +0.6 6.0 DNA-binding transcriptional activator (NCBI) compare
b4679 yohP +0.6 2.6 expressed protein (RefSeq) compare
b1627 rsxA +0.6 3.3 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0661 miaB +0.6 5.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1007 b1007 +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b1936 intG +0.6 0.9 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0240 crl +0.6 4.4 DNA-binding transcriptional regulator (NCBI) compare
b2684 mprA +0.5 3.1 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3146 yraL +0.5 3.9 predicted methyltransferase (NCBI) compare
b2659 b2659 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b0623 cspE +0.5 2.1 cold shock protein E (NCBI) compare
b2006 yeeW +0.5 2.6 CP4-44 prophage; predicted protein (RefSeq) compare
b3198 kdsC +0.5 1.6 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b0263 afuB +0.5 1.2 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b1179 ycgL +0.5 1.4 conserved protein (NCBI) compare
b1630 rsxD +0.5 2.8 electron transport complex protein RnfD (NCBI) compare
b4648 ysaC +0.5 1.7 no description compare
b3261 fis +0.5 1.3 DNA-binding protein Fis (NCBI) compare
b1573 ydfC +0.5 1.3 Qin prophage; predicted protein (RefSeq) compare
b1557 cspB +0.5 1.1 Qin prophage; cold shock protein (NCBI) compare
b2444 yffM +0.5 1.5 CPZ-55 prophage; predicted protein (NCBI) compare
b3443 yrhA +0.5 0.7 orf, hypothetical protein (VIMSS) compare
b2229 b2229 +0.5 2.6 orf, hypothetical protein (VIMSS) compare
b3199 yrbK +0.5 1.1 hypothetical protein (NCBI) compare
b4443 gcvB +0.5 0.3 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b3929 rraA +0.5 3.7 ribonuclease activity regulator protein RraA (NCBI) compare
b2903 gcvP +0.4 3.9 glycine dehydrogenase (NCBI) compare
b4594 ymgJ +0.4 1.8 hypothetical protein (RefSeq) compare
b1841 yobA +0.4 1.6 hypothetical protein (NCBI) compare
b3816 corA +0.4 3.6 magnesium/nickel/cobalt transporter (NCBI) compare
b1583 ynfB +0.4 1.6 hypothetical protein (NCBI) compare
b3181 greA +0.4 2.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1950 fliR +0.4 2.1 flagellar biosynthesis protein R (NCBI) compare
b1818 manY +0.4 1.8 mannose-specific enzyme IIC component of PTS (NCBI) compare
b3523 yhjE +0.4 3.7 predicted transporter (NCBI) compare
b3166 truB +0.4 3.2 tRNA pseudouridine synthase B (NCBI) compare
b3977 tyrU +0.4 0.6 tRNA-Tyr (NCBI) compare
b1726 yniB +0.4 1.4 predicted inner membrane protein (NCBI) compare
b0806 ybiM +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1582 ynfA +0.4 1.5 hypothetical protein (NCBI) compare
b1540 ydfH +0.4 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1607 ydgC +0.4 1.3 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3979 thrT +0.4 0.3 tRNA-Thr (NCBI) compare
b4049 dusA +0.4 3.0 tRNA-dihydrouridine synthase A (RefSeq) compare
b2369 evgA +0.4 1.9 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b0388 aroL +0.4 2.9 shikimate kinase II (NCBI) compare
b3890 yiiF +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b1138 ymfE +0.4 1.2 e14 prophage; predicted inner membrane protein (NCBI) compare
b1357 ydaS +0.4 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4066 yjcF +0.4 0.3 hypothetical protein (NCBI) compare
b1595 ynfL +0.4 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1492 gadC +0.4 2.7 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b4595 yciY +0.4 1.6 hypothetical protein (RefSeq) compare
b2960 yggH +0.4 2.5 tRNA(m7G46)-methyltransferase (NCBI) compare
b2617 smpA +0.4 2.0 small membrane protein A (VIMSS) compare
b1831 proQ +0.4 2.1 putative solute/DNA competence effector (NCBI) compare
b1259 yciG +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b4203 rplI +0.4 2.4 50S ribosomal protein L9 (NCBI) compare
b2236 yfaE +0.4 1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1487 ddpA +0.4 1.8 D-ala-D-a la transporter subunit (NCBI) compare
b2915 yqfE +0.4 1.7 hypothetical protein (RefSeq) compare
b3495 uspA +0.4 3.4 universal stress global response regulator (NCBI) compare
b1609 rstB +0.4 2.3 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b4181 yjfI +0.4 1.7 hypothetical protein (NCBI) compare
b2743 pcm +0.4 1.8 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b4100 phnH +0.4 2.3 carbon-phosphorus lyase complex subunit (NCBI) compare
b1667 ydhR +0.4 1.1 hypothetical protein (NCBI) compare
b1306 pspC +0.3 1.6 DNA-binding transcriptional activator (NCBI) compare
b2346 vacJ +0.3 2.7 predicted lipoprotein (NCBI) compare
b3028 mdaB +0.3 2.6 NADPH quinone reductase (NCBI) compare
b1822 rrmA +0.3 1.6 23S rRNA m1G745 methyltransferase (NCBI) compare
b1361 ydaW +0.3 0.3 orf, hypothetical protein (VIMSS) compare
b1358 ydaT +0.3 0.9 Rac prophage; predicted protein (NCBI) compare
b1125 potB +0.3 1.3 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1743 spy +0.3 1.3 envelope stress induced periplasmic protein (NCBI) compare
b2668 ygaP +0.3 2.3 predicted inner membrane protein with hydrolase activity (NCBI) compare
b0136 yadK +0.3 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b4071 nrfB +0.3 1.4 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b4148 sugE +0.3 2.0 suppresses groEL, may be chaperone (VIMSS) compare
b4532 yncN +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2061 wzb +0.3 1.2 protein-tyrosine phosphatase (NCBI) compare
b1669 ydhT +0.3 1.2 hypothetical protein (NCBI) compare
b1632 rsxE +0.3 2.4 NADH-ubiquinone oxidoreductase (NCBI) compare
b4431 rprA +0.3 1.0 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b0500 ybbD +0.3 0.2 hypothetical protein (RefSeq) compare
b1821 b1821 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b4536 yobH +0.3 1.5 hypothetical protein (NCBI) compare
b4371 rsmC +0.3 2.6 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1960 vsr +0.3 1.3 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b4642 yoeD +0.3 1.0 no description compare
b0535 fimZ +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0821 ybiU +0.3 2.1 hypothetical protein (NCBI) compare
b0461 ybaJ +0.3 2.2 hypothetical protein (NCBI) compare
b1493 gadB +0.3 0.5 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1758 b1758 +0.3 0.7 putative cytochrome oxidase (VIMSS) compare
b1243 oppA +0.3 2.5 oligopeptide transporter subunit (NCBI) compare
b4279 yjhB +0.3 0.2 putative transport protein (VIMSS) compare
b1218 chaC +0.3 1.5 regulatory protein for cation transport (RefSeq) compare
b1948 fliP +0.3 1.5 flagellar biosynthesis protein P (NCBI) compare
b0310 ykgH +0.3 0.7 predicted inner membrane protein (NCBI) compare
b4653 yicT +0.3 0.8 no description compare
b0382 yaiB +0.3 2.4 hypothetical protein (NCBI) compare
b2181 yejG +0.3 1.6 hypothetical protein (NCBI) compare
b3516 gadX +0.3 2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2560 yfhB +0.3 2.3 orf, hypothetical protein (VIMSS) compare
b1247 oppF +0.3 2.2 oligopeptide transporter subunit (NCBI) compare
b4391 yjjK +0.3 2.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4513 kdpF +0.3 1.4 potassium ion accessory transporter subunit (NCBI) compare
b4557 yidD +0.3 1.6 hypothetical protein (NCBI) compare
b1849 purT +0.3 1.6 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1942 fliJ +0.3 0.9 flagellar biosynthesis chaperone (NCBI) compare
b2968 yghD +0.3 1.5 predicted secretion pathway M-type protein, membrane anchored (NCBI) compare
b3686 ibpB +0.3 1.9 heat shock chaperone (NCBI) compare
b3884 yihW +0.3 1.8 putative DEOR-type transcriptional regulator (VIMSS) compare
b4545 ypdJ +0.3 1.2 no description compare
b4196 ulaD +0.3 1.5 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI) compare
b1884 cheR +0.3 1.5 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b4370 leuQ +0.3 0.9 tRNA-Leu (NCBI) compare
b2637 yfjT +0.3 1.2 CP4-57 prophage; predicted protein (NCBI) compare
b4450 arcZ +0.3 1.1 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1186 nhaB +0.3 2.1 NhaB sodium/proton transporter (Katherine Huang) compare
b0303 ykgI +0.3 0.3 orf, hypothetical protein (VIMSS) compare
b3688 yidQ +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2679 proX +0.3 1.7 glycine betaine transporter subunit (NCBI) compare
b3611 yibN +0.3 1.5 predicted rhodanese-related sulfurtransferase (NCBI) compare
b1380 ldhA +0.3 1.1 D-lactate dehydrogenase (NCBI) compare
b3468 yhhN +0.3 2.4 conserved inner membrane protein (NCBI) compare
b4406 yaeP +0.3 1.3 hypothetical protein (NCBI) compare
b0982 yccY +0.3 1.9 putative phosphatase (VIMSS) compare
b3442 yhhZ +0.3 0.2 hypothetical protein (NCBI) compare
b2570 rseC +0.3 1.8 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b3995 rsd +0.3 1.9 stationary phase protein, binds sigma 70 RNA polymerase subunit (NCBI) compare
b4095 phnM +0.3 2.1 carbon-phosphorus lyase complex subunit (NCBI) compare
b4376 osmY +0.3 2.2 periplasmic protein (NCBI) compare
b2474 ypfI +0.3 2.3 predicted hydrolase (NCBI) compare
b0864 artP +0.3 1.4 arginine transporter subunit (NCBI) compare
b1172 ymgG +0.3 1.8 conserved protein, UPF0757 family (RefSeq) compare
b4694 yagP +0.3 1.7 no description compare
b0933 ssuB +0.3 1.4 alkanesulfonate transporter subunit (NCBI) compare
b1305 pspB +0.3 0.8 phage shock protein B (NCBI) compare
b2141 yohJ +0.3 1.6 hypothetical protein (NCBI) compare
b1301 puuB +0.3 2.1 gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI) compare
b1848 yebG +0.3 0.7 conserved protein regulated by LexA (NCBI) compare
b1343 dbpA +0.3 1.8 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b1300 puuC +0.3 1.3 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b2512 yfgL +0.3 1.4 protein assembly complex, lipoprotein component (NCBI) compare
b2953 yggU +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b2502 ppx +0.3 2.1 exopolyphosphatase (NCBI) compare
b2768 ygcP +0.3 1.0 predicted anti-terminator regulatory protein (NCBI) compare
b4050 yjbO +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4163 glyV +0.3 0.8 tRNA-Gly (NCBI) compare
b0232 yafN +0.3 0.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1921 fliZ +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2904 gcvH +0.3 0.6 glycine cleavage system protein H (NCBI) compare
b2003 yeeT +0.3 1.6 CP4-44 prophage; predicted protein (NCBI) compare
b1279 yciS +0.3 0.6 conserved inner membrane protein (NCBI) compare
b0946 ycbW +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2504 yfgG +0.3 0.6 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1245 oppC +0.3 2.0 oligopeptide transporter subunit (NCBI) compare
b0238 gpt +0.3 1.0 xanthine phosphoribosyltransferase (NCBI) compare
b2759 ygcK +0.3 1.8 hypothetical protein (NCBI) compare
b1148 ymfM +0.3 1.2 e14 prophage; predicted protein (NCBI) compare
b4135 yjdC +0.2 1.8 orf, hypothetical protein (VIMSS) compare
b1640 ydhH +0.2 1.5 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b1534 ydeE +0.2 1.6 predicted transporter (NCBI) compare
b0131 panD +0.2 1.6 aspartate 1-decarboxylase precursor (NCBI) compare
b3858 yihD +0.2 1.0 hypothetical protein (NCBI) compare
b2319 usg +0.2 1.3 hypothetical protein (NCBI) compare
b1257 yciE +0.2 0.1 hypothetical protein (NCBI) compare
b3889 yiiE +0.2 0.8 predicted transcriptional regulator (RefSeq) compare
b3472 b3472 +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b4373 rimI +0.2 0.8 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b2047 wcaJ +0.2 1.2 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b2664 ygaE +0.2 1.9 putative transcriptional regulator (VIMSS) compare
b2170 setB +0.2 1.1 lactose/glucose efflux system (NCBI) compare
b4032 malG +0.2 1.8 maltose transporter subunit (NCBI) compare
b1920 fliY +0.2 0.9 cystine transporter subunit (NCBI) compare
b2528 iscA +0.2 0.4 iron-sulfur cluster assembly protein (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms