Experiment set53IT040 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.06 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b3929 rraA +3.7 32.8 ribonuclease activity regulator protein RraA (NCBI) compare
b1085 b1085 +2.7 3.8 orf, hypothetical protein (VIMSS) compare
b2576 srmB +2.3 9.5 ATP-dependent RNA helicase (NCBI) compare
b0754 aroG +2.1 16.8 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b2934 cmtB +2.0 3.1 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2688 gshA +2.0 14.7 glutamate--cysteine ligase (NCBI) compare
b0882 clpA +1.8 15.2 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b0755 gpmA +1.5 11.5 phosphoglyceromutase (NCBI) compare
b4313 fimE +1.5 11.2 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2684 mprA +1.5 5.4 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0388 aroL +1.4 10.8 shikimate kinase II (NCBI) compare
b3199 yrbK +1.4 4.8 hypothetical protein (NCBI) compare
b1954 dsrA +1.3 5.4 regulatory antisense RNA (NCBI) compare
b1642 slyA +1.2 4.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3146 yraL +1.2 8.3 predicted methyltransferase (NCBI) compare
b3210 arcB +1.2 8.8 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1831 proQ +1.1 3.7 putative solute/DNA competence effector (NCBI) compare
b3198 kdsC +1.1 4.9 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b0082 mraW +1.1 7.0 S-adenosyl-methyltransferase (NCBI) compare
b0955 ycbZ +1.1 9.3 predicted peptidase (NCBI) compare
b0830 yliB +1.1 8.4 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b2007 yeeX +1.1 6.4 conserved protein (RefSeq) compare
b3162 deaD +1.1 6.2 inducible ATP-independent RNA helicase (VIMSS) compare
b4603 rseX +1.0 1.3 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1861 ruvA +0.9 0.9 Holliday junction DNA helicase motor protein (NCBI) compare
b2620 smpB +0.9 1.6 SsrA-binding protein (NCBI) compare
b2741 rpoS +0.9 7.6 RNA polymerase sigma factor (NCBI) compare
b2217 rcsB +0.9 6.4 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1628 rsxB +0.9 2.6 electron transport complex protein RnfB (NCBI) compare
b2948 yqgE +0.9 3.1 orf, hypothetical protein (VIMSS) compare
b1936 intG +0.9 1.3 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1482 osmC +0.9 3.3 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3206 npr +0.9 4.1 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4045 yjbJ +0.8 2.1 predicted stress response protein (NCBI) compare
b4552 yrhC +0.8 1.4 no description compare
b1269 rluB +0.8 4.0 23S rRNA pseudouridylate synthase (NCBI) compare
b2947 gshB +0.8 4.0 glutathione synthetase (NCBI) compare
b3816 corA +0.8 5.9 magnesium/nickel/cobalt transporter (NCBI) compare
b0832 yliD +0.7 6.3 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3163 nlpI +0.7 3.6 hypothetical protein (NCBI) compare
b0831 yliC +0.7 4.1 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1108 ycfP +0.7 3.4 orf, hypothetical protein (VIMSS) compare
b3755 yieP +0.7 5.5 predicted transcriptional regulator (NCBI) compare
b3753 rbsR +0.7 6.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2421 cysM +0.7 4.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0998 torD +0.7 1.6 chaperone protein TorD (NCBI) compare
b2829 ptsP +0.7 6.2 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1492 gadC +0.7 4.9 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b0829 gsiA +0.7 5.8 fused predicted peptide transport subunits of ABC superfamily: ATP-binding components (RefSeq) compare
b2786 barA +0.7 6.5 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b3628 rfaB +0.6 5.0 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1573 ydfC +0.6 1.8 Qin prophage; predicted protein (RefSeq) compare
b1627 rsxA +0.6 2.9 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2571 rseB +0.6 4.4 periplasmic negative regulator of sigmaE (NCBI) compare
b4227 ytfQ +0.6 2.9 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1630 rsxD +0.6 2.8 electron transport complex protein RnfD (NCBI) compare
b1591 b1591 +0.6 2.1 putative oxidoreductase component (VIMSS) compare
b1914 uvrY +0.6 4.1 response regulator (NCBI) compare
b3676 yidH +0.6 3.7 conserved inner membrane protein (NCBI) compare
b0976 hyaE +0.6 2.7 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b4133 cadC +0.6 5.2 DNA-binding transcriptional activator (NCBI) compare
b2570 rseC +0.6 3.5 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0122 yacC +0.6 3.2 orf, hypothetical protein (VIMSS) compare
b0705 ybfL +0.6 0.6 putative receptor protein (VIMSS) compare
b2911 ssrS +0.6 1.2 6S regulatory RNA (NCBI) compare
b1632 rsxE +0.6 4.3 NADH-ubiquinone oxidoreductase (NCBI) compare
b2618 b2618 +0.6 2.7 orf, hypothetical protein (VIMSS) compare
b4450 arcZ +0.6 3.5 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1824 yobF +0.5 2.2 hypothetical protein (NCBI) compare
b3157 yhbT +0.5 2.9 predicted lipid carrier protein (NCBI) compare
b4256 yjgM +0.5 4.3 predicted acetyltransferase (NCBI) compare
b0759 galE +0.5 2.9 UDP-galactose-4-epimerase (VIMSS) compare
b0240 crl +0.5 4.6 DNA-binding transcriptional regulator (NCBI) compare
b1150 ymfR +0.5 0.9 e14 prophage; predicted protein (NCBI) compare
b1558 cspF +0.5 2.2 Qin prophage; cold shock protein (NCBI) compare
b2528 iscA +0.5 1.4 iron-sulfur cluster assembly protein (NCBI) compare
b3341 rpsG +0.5 1.3 30S ribosomal protein S7 (NCBI) compare
b2136 yohD +0.5 3.0 orf, hypothetical protein (VIMSS) compare
b2749 ygbE +0.5 4.5 conserved inner membrane protein (NCBI) compare
b1164 ycgZ +0.5 1.1 hypothetical protein (NCBI) compare
b1830 prc +0.5 2.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3349 slyD +0.5 3.7 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b1187 fadR +0.5 2.5 fatty acid metabolism regulator (NCBI) compare
b0382 yaiB +0.5 3.9 hypothetical protein (NCBI) compare
b4679 yohP +0.5 2.4 expressed protein (RefSeq) compare
b1629 rsxC +0.5 3.5 electron transport complex protein RnfC (NCBI) compare
b3261 fis +0.5 1.4 DNA-binding protein Fis (NCBI) compare
b2012 yeeD +0.5 1.3 hypothetical protein (NCBI) compare
b4012 yjaB +0.5 1.7 predicted acetyltransferase (NCBI) compare
b0178 hlpA +0.5 2.7 periplasmic chaperone (NCBI) compare
b3009 yghB +0.5 3.6 conserved inner membrane protein (NCBI) compare
b0436 tig +0.5 3.6 trigger factor (NCBI) compare
b0661 miaB +0.5 4.0 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b2145 yeiS +0.5 2.8 predicted inner membrane protein (NCBI) compare
b1837 yebW +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b0613 citG +0.5 1.4 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b2189 proL +0.5 1.6 tRNA-Pro (NCBI) compare
b0864 artP +0.5 2.4 arginine transporter subunit (NCBI) compare
b3871 bipA +0.5 3.8 GTP-binding protein (NCBI) compare
b1422 ydcI +0.5 2.6 putative transcriptional regulator LYSR-type (VIMSS) compare
b1325 ycjG +0.5 2.0 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b1761 gdhA +0.5 3.5 glutamate dehydrogenase (NCBI) compare
b0051 ksgA +0.5 2.0 dimethyladenosine transferase (NCBI) compare
b0490 ybbL +0.5 3.2 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0925 ycbB +0.4 3.9 predicted carboxypeptidase (NCBI) compare
b0225 yafQ +0.4 2.1 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1999 yeeP +0.4 2.6 putative histone (VIMSS) compare
b1919 yedO +0.4 2.4 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b4451 ryhB +0.4 1.4 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1607 ydgC +0.4 0.9 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2635 ypjK +0.4 1.9 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2904 gcvH +0.4 0.7 glycine cleavage system protein H (NCBI) compare
b4590 ybfK +0.4 0.6 hypothetical protein (RefSeq) compare
b2485 hyfE +0.4 2.3 hydrogenase 4, membrane subunit (NCBI) compare
b3523 yhjE +0.4 3.7 predicted transporter (NCBI) compare
b4190 yjfP +0.4 3.2 predicted hydrolase (NCBI) compare
b1823 cspC +0.4 1.7 stress protein, member of the CspA-family (NCBI) compare
b1623 add +0.4 1.4 adenosine deaminase (NCBI) compare
b3181 greA +0.4 2.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4246 pyrL +0.4 0.9 pyrBI operon leader peptide (NCBI) compare
b2903 gcvP +0.4 3.1 glycine dehydrogenase (NCBI) compare
b2356 yfdM +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4049 dusA +0.4 2.9 tRNA-dihydrouridine synthase A (RefSeq) compare
b2679 proX +0.4 1.7 glycine betaine transporter subunit (NCBI) compare
b4557 yidD +0.4 2.1 hypothetical protein (NCBI) compare
b3672 ivbL +0.4 1.5 ilvB operon leader peptide (NCBI) compare
b1869 yecN +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b0641 rlpB +0.4 1.3 minor lipoprotein (NCBI) compare
b3627 rfaI +0.4 3.1 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2960 yggH +0.4 2.9 tRNA(m7G46)-methyltransferase (NCBI) compare
b0294 ykgK +0.4 1.9 predicted regulator (NCBI) compare
b3780 rhlB +0.4 3.3 ATP-dependent RNA helicase (NCBI) compare
b1186 nhaB +0.4 2.9 NhaB sodium/proton transporter (Katherine Huang) compare
b2050 wcaI +0.4 2.8 predicted glycosyl transferase (NCBI) compare
b1355 b1355 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b4011 yjaA +0.4 1.2 hypothetical protein (NCBI) compare
b3933 ftsN +0.4 2.8 essential cell division protein (NCBI) compare
b3292 zntR +0.4 2.0 zinc-responsive transcriptional regulator (NCBI) compare
b3133 agaV +0.4 1.0 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b3995 rsd +0.4 2.4 stationary phase protein, binds sigma 70 RNA polymerase subunit (NCBI) compare
b2480 bcp +0.4 2.6 thioredoxin-dependent thiol peroxidase (NCBI) compare
b2363 yfdT +0.4 1.4 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4431 rprA +0.4 1.3 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2464 talA +0.4 1.8 transaldolase A (NCBI) compare
b3503 arsC +0.4 2.3 arsenate reductase (NCBI) compare
b2003 yeeT +0.4 1.5 CP4-44 prophage; predicted protein (NCBI) compare
b4406 yaeP +0.4 1.7 hypothetical protein (NCBI) compare
b2668 ygaP +0.4 2.7 predicted inner membrane protein with hydrolase activity (NCBI) compare
b2667 ygaV +0.4 2.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1818 manY +0.4 1.1 mannose-specific enzyme IIC component of PTS (NCBI) compare
b3071 yqjI +0.4 1.9 predicted transcriptional regulator (NCBI) compare
b3512 GadE +0.4 1.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4181 yjfI +0.4 1.5 hypothetical protein (NCBI) compare
b3516 gadX +0.4 3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3002 yqhA +0.4 0.7 hypothetical protein (NCBI) compare
b4071 nrfB +0.4 1.7 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b0131 panD +0.4 2.1 aspartate 1-decarboxylase precursor (NCBI) compare
b1233 ychJ +0.4 1.6 hypothetical protein (NCBI) compare
b3611 yibN +0.4 1.8 predicted rhodanese-related sulfurtransferase (NCBI) compare
b0310 ykgH +0.4 0.8 predicted inner membrane protein (NCBI) compare
b0617 citD +0.3 1.4 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b3468 yhhN +0.3 3.0 conserved inner membrane protein (NCBI) compare
b3260 dusB +0.3 2.1 tRNA-dihydrouridine synthase B (NCBI) compare
b0143 pcnB +0.3 2.0 poly(A) polymerase I (VIMSS) compare
b3812 yigB +0.3 2.5 predicted hydrolase (NCBI) compare
b4631 ykgQ +0.3 0.7 no description compare
b2512 yfgL +0.3 1.4 protein assembly complex, lipoprotein component (NCBI) compare
b1730 ydjO +0.3 0.6 predicted protein (RefSeq) compare
b4585 sroB +0.3 0.7 Novel sRNA, function unknown (RefSeq) compare
b0279 yagM +0.3 0.2 CP4-6 prophage; predicted protein (NCBI) compare
b3237 argR +0.3 1.5 arginine repressor (NCBI) compare
b4559 yjdO +0.3 1.6 hypothetical protein (NCBI) compare
b1038 csgF +0.3 1.5 predicted transport protein (NCBI) compare
b1725 yniA +0.3 2.1 predicted phosphotransferase/kinase (NCBI) compare
b4215 ytfI +0.3 0.8 hypothetical protein (NCBI) compare
b0018 mokC +0.3 0.8 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4426 isrA +0.3 1.3 unknown RNA (NCBI) compare
b4651 yibW +0.3 0.2 no description compare
b1287 yciW +0.3 2.1 putative oxidoreductase (VIMSS) compare
b1263 trpD +0.3 0.5 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b3875 ompL +0.3 1.2 predicted outer membrane porin L (NCBI) compare
b4622 ytcA +0.3 1.8 hypothetical protein (RefSeq) compare
b0425 panE +0.3 2.7 2-dehydropantoate 2-reductase (NCBI) compare
b1295 ymjA +0.3 1.3 hypothetical protein (NCBI) compare
b2783 chpR +0.3 1.3 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b0851 nfsA +0.3 1.5 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1476 fdnI +0.3 0.6 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2099 yegU +0.3 2.0 predicted hydrolase (NCBI) compare
b1343 dbpA +0.3 2.1 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b1007 b1007 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1984 asnW +0.3 0.6 tRNA-Asn (NCBI) compare
b2768 ygcP +0.3 1.1 predicted anti-terminator regulatory protein (NCBI) compare
b1110 ycfJ +0.3 2.1 hypothetical protein (NCBI) compare
b1922 fliA +0.3 2.0 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS) compare
b2502 ppx +0.3 2.2 exopolyphosphatase (NCBI) compare
b1408 b1408 +0.3 2.0 probable enzyme (VIMSS) compare
b2266 elaB +0.3 1.8 hypothetical protein (NCBI) compare
b2061 wzb +0.3 1.1 protein-tyrosine phosphatase (NCBI) compare
b1674 ydhY +0.3 2.1 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b1442 ydcU +0.3 1.6 predicted spermidine/putrescine transporter subunit (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms