Experiment set53IT040 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.06 mM 48 hr

Group: stress
Media: M9 + Methotrexate (0.06 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 4 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms

SEED Subsystems

Subsystem #Specific
Chorismate Synthesis 2
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 2
Biogenesis of cytochrome c oxidases 1
CBSS-562.2.peg.5158 SK3 including 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-dehydroquinate biosynthesis I 2 2 1
chorismate biosynthesis I 7 7 2
photosynthesis light reactions 4 1 1
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) 4 1 1
superpathway of L-phenylalanine biosynthesis 10 10 2
superpathway of L-tyrosine biosynthesis 10 10 2
chorismate biosynthesis from 3-dehydroquinate 5 5 1
superpathway of photosynthetic hydrogen production 6 3 1
superpathway of L-tryptophan biosynthesis 13 13 2
superpathway of aromatic amino acid biosynthesis 18 18 2
chorismate biosynthesis II (archaea) 12 8 1
oxygenic photosynthesis 17 11 1
ethene biosynthesis V (engineered) 25 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1
superpathway of chorismate metabolism 59 54 2