Experiment set53IT038 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 1.98 mM 24 hr

Group: stress
Media: M9 + Methotrexate (1.98 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 15 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter oligopeptide (TC 3.A.1.5.1) 5
Folate Biosynthesis 2
Deoxyribose and Deoxynucleoside Catabolism 1
Nitrate and nitrite ammonification 1
Sex pheromones in Enterococcus faecalis and other Firmicutes 1
cAMP signaling in bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
tetrahydromonapterin biosynthesis 4 3 2
2-deoxy-α-D-ribose 1-phosphate degradation 3 3 1
tetrahydrofolate biosynthesis I 3 3 1
dTMP de novo biosynthesis (mitochondrial) 3 3 1
2-deoxy-D-ribose degradation I 3 3 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 6 1
superpathway of purine deoxyribonucleosides degradation 7 7 1
folate transformations III (E. coli) 9 9 1
superpathway of tetrahydrofolate biosynthesis 10 9 1
folate transformations II (plants) 11 10 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 11 1
superpathway of chorismate metabolism 59 54 1