Experiment set53IT037 for Escherichia coli BW25113
M9 with Methotrexate 0.06 mM 24 hr
Group: stressMedia: M9 + Methotrexate (0.06 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 1 genes in this experiment
For stress Methotrexate in Escherichia coli BW25113
For stress Methotrexate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biogenesis of cytochrome c oxidases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) | 4 | 1 | 1 |
| photosynthesis light reactions | 4 | 1 | 1 |
| superpathway of photosynthetic hydrogen production | 6 | 3 | 1 |
| oxygenic photosynthesis | 17 | 11 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |