Experiment set53IT030 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.1 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +3.2 23.6 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1954 dsrA +1.8 9.0 regulatory antisense RNA (NCBI) compare
b1823 cspC +1.7 4.4 stress protein, member of the CspA-family (NCBI) compare
b4402 yjjY +1.6 3.1 hypothetical protein (NCBI) compare
b2217 rcsB +1.3 9.0 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4133 cadC +1.1 9.7 DNA-binding transcriptional activator (NCBI) compare
b4603 rseX +1.0 1.3 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2531 iscR +1.0 2.7 DNA-binding transcriptional repressor (NCBI) compare
b4246 pyrL +1.0 2.4 pyrBI operon leader peptide (NCBI) compare
b2659 b2659 +1.0 2.5 orf, hypothetical protein (VIMSS) compare
b3443 yrhA +0.9 2.1 orf, hypothetical protein (VIMSS) compare
b2741 rpoS +0.9 6.4 RNA polymerase sigma factor (NCBI) compare
b1709 btuD +0.9 1.6 vitamin B12-transporter ATPase (NCBI) compare
b4215 ytfI +0.8 2.1 hypothetical protein (NCBI) compare
b3753 rbsR +0.8 6.9 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b3755 yieP +0.8 5.5 predicted transcriptional regulator (NCBI) compare
b1506 yneL +0.8 1.7 predicted transcriptional regulator (NCBI) compare
b1831 proQ +0.7 4.1 putative solute/DNA competence effector (NCBI) compare
b1848 yebG +0.7 2.0 conserved protein regulated by LexA (NCBI) compare
b3206 npr +0.7 3.8 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1253 yciA +0.7 2.3 predicted hydrolase (NCBI) compare
b4585 sroB +0.7 1.6 Novel sRNA, function unknown (RefSeq) compare
b1400 paaY +0.7 2.2 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b3146 yraL +0.7 4.7 predicted methyltransferase (NCBI) compare
b0240 crl +0.7 5.6 DNA-binding transcriptional regulator (NCBI) compare
b2502 ppx +0.7 5.1 exopolyphosphatase (NCBI) compare
b1642 slyA +0.7 2.8 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1245 oppC +0.7 4.2 oligopeptide transporter subunit (NCBI) compare
b2181 yejG +0.6 3.0 hypothetical protein (NCBI) compare
b2846 yqeH +0.6 0.7 orf, hypothetical protein (VIMSS) compare
b2012 yeeD +0.6 1.6 hypothetical protein (NCBI) compare
b1607 ydgC +0.6 1.8 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2189 proL +0.6 2.2 tRNA-Pro (NCBI) compare
b3512 GadE +0.6 3.2 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0232 yafN +0.6 0.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4045 yjbJ +0.6 1.8 predicted stress response protein (NCBI) compare
b1936 intG +0.6 0.9 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4593 ymgI +0.6 1.0 hypothetical protein (RefSeq) compare
b2829 ptsP +0.6 5.5 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1658 purR +0.6 3.9 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3261 fis +0.6 1.6 DNA-binding protein Fis (NCBI) compare
b2007 yeeX +0.6 1.4 conserved protein (RefSeq) compare
b1134 ymfB +0.6 1.5 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1839 yebY +0.6 2.0 hypothetical protein (NCBI) compare
b0234 yafP +0.6 1.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1765 ydjA +0.6 0.8 predicted oxidoreductase (NCBI) compare
b3933 ftsN +0.6 4.2 essential cell division protein (NCBI) compare
b3516 gadX +0.6 4.9 DNA-binding transcriptional dual regulator (NCBI) compare
b1361 ydaW +0.5 0.5 orf, hypothetical protein (VIMSS) compare
b1358 ydaT +0.5 1.4 Rac prophage; predicted protein (NCBI) compare
b3397 nudE +0.5 3.9 ADP-ribose diphosphatase (NCBI) compare
b3495 uspA +0.5 4.7 universal stress global response regulator (NCBI) compare
b2479 gcvR +0.5 2.8 transcriptional regulation of gcv operon (VIMSS) compare
b1669 ydhT +0.5 2.5 hypothetical protein (NCBI) compare
b2343 b2343 +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b3889 yiiE +0.5 1.7 predicted transcriptional regulator (RefSeq) compare
b2637 yfjT +0.5 2.1 CP4-57 prophage; predicted protein (NCBI) compare
b4450 arcZ +0.5 2.9 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1860 ruvB +0.5 0.4 Holliday junction DNA helicase B (NCBI) compare
b2960 yggH +0.5 3.3 tRNA(m7G46)-methyltransferase (NCBI) compare
b1465 narV +0.5 1.2 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1247 oppF +0.5 2.5 oligopeptide transporter subunit (NCBI) compare
b1246 oppD +0.5 3.4 oligopeptide transporter ATP-binding component (NCBI) compare
b1179 ycgL +0.5 1.4 conserved protein (NCBI) compare
b4642 yoeD +0.5 2.0 no description compare
b1150 ymfR +0.5 0.8 e14 prophage; predicted protein (NCBI) compare
b1233 ychJ +0.5 1.3 hypothetical protein (NCBI) compare
b1984 asnW +0.5 0.9 tRNA-Asn (NCBI) compare
b3238 yhcN +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b0802 ybiJ +0.5 2.1 hypothetical protein (NCBI) compare
b1493 gadB +0.5 0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4545 ypdJ +0.5 1.9 no description compare
b0661 miaB +0.5 3.8 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3816 corA +0.5 3.6 magnesium/nickel/cobalt transporter (NCBI) compare
b3190 yrbA +0.4 2.9 orf, hypothetical protein (VIMSS) compare
b4214 cysQ +0.4 3.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0436 tig +0.4 2.6 trigger factor (NCBI) compare
b0696 kdpC +0.4 1.7 potassium-transporting ATPase subunit C (NCBI) compare
b0247 ykfG +0.4 1.1 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1600 mdtJ +0.4 1.1 multidrug efflux system transporter (NCBI) compare
b4066 yjcF +0.4 0.3 hypothetical protein (NCBI) compare
b1244 oppB +0.4 3.0 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b4590 ybfK +0.4 0.6 hypothetical protein (RefSeq) compare
b3098 yqjD +0.4 2.3 hypothetical protein (NCBI) compare
b0388 aroL +0.4 2.3 shikimate kinase II (NCBI) compare
b1151 ymfO +0.4 2.5 e14 prophage; conserved protein (RefSeq) compare
b4071 nrfB +0.4 2.0 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b3779 gpp +0.4 3.7 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b1670 ydhU +0.4 1.8 predicted cytochrome (NCBI) compare
b1726 yniB +0.4 0.8 predicted inner membrane protein (NCBI) compare
b3429 glgA +0.4 3.4 glycogen synthase (NCBI) compare
b2528 iscA +0.4 0.8 iron-sulfur cluster assembly protein (NCBI) compare
b0580 ybdJ +0.4 1.0 predicted inner membrane protein (NCBI) compare
b1949 fliQ +0.4 1.9 flagellar biosynthesis protein Q (NCBI) compare
b2266 elaB +0.4 1.7 hypothetical protein (NCBI) compare
b1221 narL +0.4 2.4 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1243 oppA +0.4 2.7 oligopeptide transporter subunit (NCBI) compare
b1824 yobF +0.4 1.8 hypothetical protein (NCBI) compare
b4431 rprA +0.4 1.4 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2668 ygaP +0.4 2.5 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1891 flhC +0.4 1.9 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b4686 yshB +0.4 1.6 expressed protein (RefSeq) compare
b1647 ydhF +0.4 2.5 predicted oxidoreductase (NCBI) compare
b0447 ybaO +0.4 2.3 putative LRP-like transcriptional regulator (VIMSS) compare
b1295 ymjA +0.4 1.0 hypothetical protein (NCBI) compare
b3643 rph +0.4 2.7 ribonuclease PH (NCBI) compare
b0920 ycbC +0.4 1.1 conserved inner membrane protein (NCBI) compare
b1357 ydaS +0.4 1.2 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b2742 nlpD +0.4 2.3 predicted outer membrane lipoprotein (NCBI) compare
b0426 yajQ +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b2666 yqaE +0.4 1.7 predicted membrane protein (NCBI) compare
b1362 rzpR +0.4 1.5 putative Rac prophage endopeptidase (VIMSS) compare
b1426 ydcH +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b3766 ilvL +0.4 1.4 ilvG operon leader peptide (NCBI) compare
b3138 agaB +0.4 1.6 N-acetylgalactosamine-specific enzyme IIB component of PTS (NCBI) compare
b3507 dctR +0.4 3.2 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b0242 proB +0.4 2.2 gamma-glutamyl kinase (NCBI) compare
b0613 citG +0.4 1.9 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b3292 zntR +0.4 1.6 zinc-responsive transcriptional regulator (NCBI) compare
b2535 csiE +0.4 3.1 orf, hypothetical protein (VIMSS) compare
b1143 ymfI +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b1164 ycgZ +0.4 0.9 hypothetical protein (NCBI) compare
b1180 ycgM +0.4 1.9 predicted isomerase/hydrolase (NCBI) compare
b1627 rsxA +0.4 2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1085 b1085 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1387 maoC +0.3 2.1 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b0955 ycbZ +0.3 2.7 predicted peptidase (NCBI) compare
b1586 b1586 +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b1953 b1953 +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b2300 yfcE +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b0082 mraW +0.3 1.5 S-adenosyl-methyltransferase (NCBI) compare
b2911 ssrS +0.3 0.5 6S regulatory RNA (NCBI) compare
b3228 sspB +0.3 1.6 ClpXP protease specificity-enhancing factor (NCBI) compare
b4651 yibW +0.3 0.2 no description compare
b2506 yfgI +0.3 1.0 hypothetical protein (NCBI) compare
b1446 ydcY +0.3 1.3 hypothetical protein (NCBI) compare
b4552 yrhC +0.3 0.5 no description compare
b1422 ydcI +0.3 1.5 putative transcriptional regulator LYSR-type (VIMSS) compare
b0263 afuB +0.3 0.8 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b2448 yffQ +0.3 1.5 CPZ-55 prophage; predicted protein (RefSeq) compare
b4222 ytfP +0.3 1.3 hypothetical protein (NCBI) compare
b2291 yfbR +0.3 1.8 hypothetical protein (NCBI) compare
b1671 ydhX +0.3 1.1 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b3912 cpxR +0.3 2.3 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b0616 citE +0.3 2.2 citrate lyase beta chain (acyl lyase subunit) (VIMSS) compare
b1492 gadC +0.3 2.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1743 spy +0.3 1.4 envelope stress induced periplasmic protein (NCBI) compare
b2945 endA +0.3 2.5 DNA-specific endonuclease I (NCBI) compare
b1012 b1012 +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1537 ydeJ +0.3 1.8 competence damage-inducible protein A (NCBI) compare
b1382 ynbE +0.3 0.9 predicted lipoprotein (NCBI) compare
b2936 yggG +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.3 0.7 hypothetical protein (NCBI) compare
b2341 yfcX +0.3 2.3 fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI) compare
b1040 csgD +0.3 0.8 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1250 kch +0.3 2.2 voltage-gated potassium channel (NCBI) compare
b4124 dcuR +0.3 2.6 DNA-binding response regulator in two-component regulatory system with DcuS (NCBI) compare
b0225 yafQ +0.3 1.6 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3071 yqjI +0.3 1.7 predicted transcriptional regulator (NCBI) compare
b2455 eutE +0.3 1.6 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b0660 ybeZ +0.3 1.7 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b1355 b1355 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1443 ydcV +0.3 1.3 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1218 chaC +0.3 2.1 regulatory protein for cation transport (RefSeq) compare
b4050 yjbO +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b0243 proA +0.3 2.3 gamma-glutamyl phosphate reductase (NCBI) compare
b3017 sufI +0.3 2.4 repressor protein for FtsI (NCBI) compare
b4157 yjeN +0.3 1.7 hypothetical protein (NCBI) compare
b0357 yaiN +0.3 0.9 putative alpha helix chain (VIMSS) compare
b3468 yhhN +0.3 2.5 conserved inner membrane protein (NCBI) compare
b0999 cbpM +0.3 1.1 modulator of CbpA co-chaperone (NCBI) compare
b1487 ddpA +0.3 1.7 D-ala-D-a la transporter subunit (NCBI) compare
b4181 yjfI +0.3 1.1 hypothetical protein (NCBI) compare
b3043 ygiL +0.3 1.4 predicted fimbrial-like adhesin protein (NCBI) compare
b0792 ybhR +0.3 1.4 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4572 ylbE +0.3 1.5 no description compare
b1730 ydjO +0.3 0.5 predicted protein (RefSeq) compare
b1821 b1821 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2476 purC +0.3 1.0 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b2953 yggU +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1194 ycgR +0.3 1.7 protein involved in flagellar function (NCBI) compare
b0435 bolA +0.3 1.3 possible regulator of murein genes (VIMSS) compare
b1948 fliP +0.3 1.5 flagellar biosynthesis protein P (NCBI) compare
b3166 truB +0.3 2.1 tRNA pseudouridine synthase B (NCBI) compare
b0803 ybiI +0.3 1.4 hypothetical protein (NCBI) compare
b3934 cytR +0.3 2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b2398 yfeC +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b0864 artP +0.3 1.4 arginine transporter subunit (NCBI) compare
b1213 ychQ +0.3 1.8 predicted transcriptional regulator (NCBI) compare
b3393 yrfB +0.3 1.9 hypothetical protein (NCBI) compare
b4072 nrfC +0.3 1.8 formate-dependent nitrite reductase, 4Fe4S subunit (NCBI) compare
b2082 ogrK +0.3 1.5 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b4101 phnG +0.3 1.0 carbon-phosphorus lyase complex subunit (NCBI) compare
b1989 asnV +0.3 1.0 tRNA-Asn (NCBI) compare
b1309 ycjM +0.3 1.5 putative polysaccharide hydrolase (VIMSS) compare
b2443 yffL +0.3 1.2 CPZ-55 prophage; predicted protein (NCBI) compare
b2644 yfjY +0.3 1.2 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b2916 argP +0.3 1.8 chromosome replication initiation inhibitor protein (NCBI) compare
b1007 b1007 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b3152 yraR +0.3 1.6 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms