Experiment set53IT030 for Escherichia coli BW25113

BHIS with Methotrexate 0.1 mM 48 hr

Group: stress
Media: BHIS + Methotrexate (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Cysteine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine biosynthesis I	2	2	1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine)	4	3	1
seleno-amino acid biosynthesis (plants)	5	3	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of seleno-compound metabolism	19	6	1