Experiment set53IT029 for Escherichia coli BW25113
M9 with Methotrexate 1.98 mM 48 hr
Group: stressMedia: M9 + Methotrexate (1.98 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 12 genes in this experiment
For stress Methotrexate in Escherichia coli BW25113
For stress Methotrexate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ABC transporter oligopeptide (TC 3.A.1.5.1) | 5 |
| Nitrate and nitrite ammonification | 1 |
| Sex pheromones in Enterococcus faecalis and other Firmicutes | 1 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes: