Experiment set53IT027 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.1 mM 24 hr

200 most important genes:

  gene name fitness t score description  
b2808 gcvA -4.2 -4.1 DNA-binding transcriptional dual regulator (NCBI) compare
b3183 obgE -3.2 -2.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3860 dsbA -3.2 -8.2 periplasmic protein disulfide isomerase I (NCBI) compare
b4242 mgtA -3.2 -4.4 magnesium transporter (NCBI) compare
b0142 folK -3.2 -1.4 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1185 dsbB -3.1 -3.7 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4171 miaA -2.8 -4.2 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0683 fur -2.8 -1.9 ferric uptake regulator (NCBI) compare
b4702 mgtL -2.8 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b0222 lpcA -2.7 -1.9 phosphoheptose isomerase (NCBI) compare
b0439 lon -2.6 -3.0 DNA-binding ATP-dependent protease La (NCBI) compare
b3164 pnp -2.4 -4.9 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2312 purF -2.3 -8.0 amidophosphoribosyltransferase (NCBI) compare
b3844 fre -2.3 -9.1 NAD(P)H-flavin reductase (NCBI) compare
b1855 lpxM -2.3 -5.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3493 pitA -2.2 -8.6 phosphate transporter, low-affinity (NCBI) compare
b2318 truA -2.2 -4.3 tRNA pseudouridine synthase A (NCBI) compare
b0726 sucA -2.2 -6.9 alpha-ketoglutarate decarboxylase (NCBI) compare
b1290 sapF -2.1 -2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3052 rfaE -2.1 -2.5 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0438 clpX -2.0 -3.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4647 mokA -2.0 -1.3 no description compare
b4005 purD -2.0 -10.5 phosphoribosylamine--glycine ligase (NCBI) compare
b2912 ygfA -1.9 -3.0 putative ligase (VIMSS) compare
b0463 acrA -1.9 -12.4 multidrug efflux system (NCBI) compare
b0080 fruR -1.9 -7.6 DNA-binding transcriptional dual regulator (NCBI) compare
b3979 thrT -1.9 -0.8 tRNA-Thr (NCBI) compare
b1292 sapC -1.8 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1882 cheY -1.8 -1.3 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1291 sapD -1.8 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4414 tff -1.8 -3.5 unknown RNA (NCBI) compare
b2168 fruK -1.8 -1.5 1-phosphofructokinase (NCBI) compare
b4006 purH -1.8 -9.5 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b0630 lipB -1.8 -1.6 protein of lipoate biosynthesis (VIMSS) compare
b4375 prfC -1.8 -6.8 peptide chain release factor 3 (NCBI) compare
b3916 pfkA -1.7 -3.7 6-phosphofructokinase (NCBI) compare
b0632 dacA -1.6 -8.8 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1286 rnb -1.6 -2.9 exoribonuclease II (NCBI) compare
b1235 rssB -1.6 -5.9 response regulator of RpoS (NCBI) compare
b0400 phoR -1.6 -9.9 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b2414 cysK -1.6 -6.7 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b0051 ksgA -1.5 -5.2 dimethyladenosine transferase (NCBI) compare
b0049 apaH -1.5 -1.0 diadenosinetetraphosphatase (NCBI) compare
b0437 clpP -1.5 -1.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0522 purK -1.5 -6.9 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b4481 rffT -1.5 -3.8 4-alpha-L-fucosyltransferase (NCBI) compare
b0889 lrp -1.5 -4.3 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2501 ppk -1.4 -9.3 polyphosphate kinase (NCBI) compare
b2288 nuoA -1.4 -4.3 NADH dehydrogenase I chain A (VIMSS) compare
b1084 rne -1.4 -4.9 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2784 relA -1.4 -9.5 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2620 smpB -1.4 -1.4 SsrA-binding protein (NCBI) compare
b0145 dksA -1.4 -3.8 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4179 vacB -1.4 -4.4 putative enzyme (VIMSS) compare
b2330 yfcB -1.4 -2.6 putative adenine-specific methylase (VIMSS) compare
b4203 rplI -1.4 -5.2 50S ribosomal protein L9 (NCBI) compare
b2329 aroC -1.4 -6.9 chorismate synthase (NCBI) compare
b0200 gmhB -1.4 -0.9 hypothetical protein (NCBI) compare
b2421 cysM -1.4 -6.1 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1438 ydcQ -1.3 -2.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3621 rfaC -1.3 -1.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2512 yfgL -1.3 -5.2 protein assembly complex, lipoprotein component (NCBI) compare
b3849 trkH -1.3 -9.2 potassium transporter (NCBI) compare
b3620 rfaF -1.3 -2.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3630 rfaP -1.3 -5.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3631 rfaG -1.3 -7.7 glucosyltransferase I (NCBI) compare
b0564 appY -1.3 -1.0 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0908 aroA -1.3 -7.6 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4529 ydbJ -1.3 -1.7 hypothetical protein (NCBI) compare
b3838 tatB -1.2 -5.3 sec-independent translocase (NCBI) compare
b0688 pgm -1.2 -2.7 phosphoglucomutase (NCBI) compare
b4180 rlmB -1.2 -7.6 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b4654 yjdQ -1.2 -4.4 no description compare
b3842 rfaH -1.2 -3.4 transcriptional activator RfaH (NCBI) compare
b3867 hemN -1.2 -8.3 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b4146 yjeK -1.2 -2.0 predicted lysine aminomutase (NCBI) compare
b0226 dinJ -1.2 -4.0 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4174 hflK -1.2 -8.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3162 deaD -1.2 -4.7 inducible ATP-independent RNA helicase (VIMSS) compare
b3790 rffC -1.2 -3.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b1850 eda -1.2 -1.5 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2276 nuoN -1.1 -5.1 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3204 ptsN -1.1 -4.2 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2504 yfgG -1.1 -1.8 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3870 glnA -1.1 -2.9 glutamine synthetase (NCBI) compare
b2617 smpA -1.1 -4.3 small membrane protein A (VIMSS) compare
b0386 proC -1.1 -2.5 pyrroline-5-carboxylate reductase (NCBI) compare
b4201 priB -1.1 -3.0 primosomal replication protein N (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b2188 yejM -1.1 -7.4 predicted hydrolase, inner membrane (NCBI) compare
b2277 nuoM -1.1 -5.4 NADH dehydrogenase subunit M (NCBI) compare
b2279 nuoK -1.1 -1.9 NADH dehydrogenase subunit K (NCBI) compare
b3741 gidA -1.1 -1.9 glucose-inhibited division protein A (NCBI) compare
b2278 nuoL -1.1 -6.5 NADH dehydrogenase subunit L (NCBI) compare
b3398 yrfF -1.0 -3.2 predicted inner membrane protein (NCBI) compare
b1874 cutC -1.0 -0.7 copper homeostasis protein (NCBI) compare
b3791 rffA -1.0 -3.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2280 nuoJ -1.0 -4.4 NADH dehydrogenase subunit J (NCBI) compare
b0912 ihfB -1.0 -0.6 integration host factor subunit beta (NCBI) compare
b1567 intK -1.0 -2.7 Qin prophage; predicted protein (RefSeq) compare
b3120 yhaB -1.0 -1.5 orf, hypothetical protein (VIMSS) compare
b2897 ygfY -1.0 -4.1 hypothetical protein (NCBI) compare
b4503 yafF -1.0 -1.2 no description compare
b1090 plsX -0.9 -4.3 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2313 cvpA -0.9 -1.9 membrane protein required for colicin V production (NCBI) compare
b2282 nuoH -0.9 -2.9 NADH dehydrogenase subunit H (NCBI) compare
b3728 pstS -0.9 -2.7 phosphate transporter subunit (NCBI) compare
b2284 nuoF -0.9 -3.9 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b4175 hflC -0.9 -5.7 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b0143 pcnB -0.9 -3.8 poly(A) polymerase I (VIMSS) compare
b0767 ybhE -0.9 -3.7 6-phosphogluconolactonase (NCBI) compare
b1281 pyrF -0.9 -1.5 orotidine 5'-phosphate decarboxylase (NCBI) compare
b1334 fnr -0.9 -3.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4025 pgi -0.9 -6.2 glucose-6-phosphate isomerase (NCBI) compare
b1192 ldcA -0.9 -2.1 L,D-carboxypeptidase A (NCBI) compare
b3794 rffM -0.9 -4.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1293 sapB -0.9 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0722 sdhD -0.9 -3.1 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2906 visC -0.9 -4.7 hypothetical protein (NCBI) compare
b0675 nagD -0.8 -5.6 UMP phosphatase (NCBI) compare
b3601 mtlR -0.8 -5.5 DNA-binding repressor (NCBI) compare
b2499 purM -0.8 -3.1 phosphoribosylaminoimidazole synthetase (NCBI) compare
b1677 lpp -0.8 -1.7 murein lipoprotein (NCBI) compare
b0724 sdhB -0.8 -4.0 succinate dehydrogenase, FeS subunit (NCBI) compare
b0928 aspC -0.8 -5.4 aspartate aminotransferase, PLP-dependent (NCBI) compare
b3778 rep -0.8 -4.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2569 lepA -0.8 -2.8 GTP-binding protein LepA (NCBI) compare
b1258 yciF -0.8 -1.0 hypothetical protein (NCBI) compare
b1729 ydjN -0.8 -3.5 predicted transporter (NCBI) compare
b3930 menA -0.8 -6.9 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1551 b1551 -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b2951 yggS -0.8 -3.8 predicted enzyme (NCBI) compare
b2557 purL -0.8 -5.6 phosphoribosylformylglycinamidine synthase (NCBI) compare
b3742 mioC -0.8 -4.0 flavodoxin (NCBI) compare
b0033 carB -0.8 -2.9 carbamoyl-phosphate synthase large subunit (NCBI) compare
b2340 sixA -0.8 -3.1 phosphohistidine phosphatase (NCBI) compare
b3786 rffE -0.8 -5.6 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0882 clpA -0.8 -6.1 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b3205 yhbJ -0.8 -2.8 hypothetical protein (NCBI) compare
b2809 b2809 -0.8 -3.2 orf, hypothetical protein (VIMSS) compare
b0721 sdhC -0.8 -3.2 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b0237 pepD -0.8 -5.6 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1753 ynjA -0.7 -1.3 hypothetical protein (NCBI) compare
b1871 yecP -0.7 -3.3 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b3082 ygjM -0.7 -0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0723 sdhA -0.7 -5.8 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3636 rpmG -0.7 -2.0 50S ribosomal protein L33 (NCBI) compare
b0053 surA -0.7 -0.7 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2287 nuoB -0.7 -2.3 NADH dehydrogenase subunit B (NCBI) compare
b3494 yhiO -0.7 -3.7 universal stress protein UspB (NCBI) compare
b3247 cafA -0.7 -5.5 bundles of cytoplasmic filaments (VIMSS) compare
b3129 sohA -0.7 -2.7 predicted regulator (NCBI) compare
b0429 cyoD -0.7 -2.7 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b3545 proK -0.7 -2.1 tRNA-Pro (NCBI) compare
b3414 gntY -0.7 -2.9 predicted gluconate transport associated protein (NCBI) compare
b3764 yifE -0.7 -3.1 hypothetical protein (NCBI) compare
b3387 dam -0.7 -3.6 DNA adenine methylase (NCBI) compare
b3787 rffD -0.7 -4.3 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1731 cedA -0.7 -3.2 cell division modulator (RefSeq) compare
b2940 yqgC -0.7 -2.3 hypothetical protein (NCBI) compare
b2283 nuoG -0.7 -3.9 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b3236 mdh -0.7 -4.5 malate dehydrogenase (NCBI) compare
b2017 yefM -0.7 -0.6 orf, hypothetical protein (VIMSS) compare
b2113 mrp -0.7 -3.0 putative ATPase (VIMSS) compare
b1686 ydiI -0.7 -1.4 hypothetical protein (NCBI) compare
b2904 gcvH -0.7 -1.6 glycine cleavage system protein H (NCBI) compare
b2051 wcaH -0.7 -2.1 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0523 purE -0.7 -1.7 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b0167 glnD -0.7 -3.0 PII uridylyl-transferase (NCBI) compare
b4000 hupA -0.6 -3.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1426 ydcH -0.6 -1.5 orf, hypothetical protein (VIMSS) compare
b3642 pyrE -0.6 -1.3 orotate phosphoribosyltransferase (NCBI) compare
b4573 insZ -0.6 -3.3 no description compare
b0926 ycbK -0.6 -3.6 hypothetical protein (NCBI) compare
b0009 mog -0.6 -4.4 molybdenum cofactor biosynthesis protein (NCBI) compare
b2905 gcvT -0.6 -3.9 glycine cleavage system aminomethyltransferase T (NCBI) compare
b3627 rfaI -0.6 -4.5 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3911 cpxA -0.6 -3.3 two-component sensor protein (NCBI) compare
b0113 pdhR -0.6 -2.6 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b4480 hdfR -0.6 -4.1 transcriptional regulator HdfR (NCBI) compare
b0902 pflA -0.6 -3.7 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b1075 flgD -0.6 -3.0 flagellar basal body rod modification protein D (NCBI) compare
b1982 amn -0.6 -4.7 AMP nucleosidase (NCBI) compare
b3619 rfaD -0.6 -0.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1171 b1171 -0.6 -1.6 orf, hypothetical protein (VIMSS) compare
b3652 recG -0.6 -4.3 ATP-dependent DNA helicase (NCBI) compare
b1323 tyrR -0.6 -2.7 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b1984 asnW -0.6 -1.0 tRNA-Asn (NCBI) compare
b0008 talB -0.6 -4.0 transaldolase B (NCBI) compare
b0197 metQ -0.6 -3.9 DL-methionine transporter subunit (NCBI) compare
b0693 speF -0.6 -3.7 ornithine decarboxylase isozyme, inducible (NCBI) compare
b0783 moaC -0.6 -3.2 molybdenum cofactor biosynthesis protein C (NCBI) compare
b2281 nuoI -0.6 -1.9 NADH dehydrogenase subunit I (NCBI) compare
b1540 ydfH -0.6 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2699 recA -0.6 -0.4 recombinase A (NCBI) compare
b2683 ygaH -0.6 -2.0 predicted inner membrane protein (NCBI) compare
b4390 nadR -0.6 -4.5 probable nadAB transcriptional regulator (VIMSS) compare
b0948 ycbY -0.6 -4.0 predicted methyltransferase (NCBI) compare
b0232 yafN -0.6 -0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3281 aroE -0.6 -4.5 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms