Experiment set53IT027 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.1 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +3.4 24.1 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1954 dsrA +2.1 11.6 regulatory antisense RNA (NCBI) compare
b2741 rpoS +2.1 17.7 RNA polymerase sigma factor (NCBI) compare
b1823 cspC +1.7 6.6 stress protein, member of the CspA-family (NCBI) compare
b2217 rcsB +1.5 11.5 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4402 yjjY +1.5 3.4 hypothetical protein (NCBI) compare
b2531 iscR +1.5 2.1 DNA-binding transcriptional repressor (NCBI) compare
b1642 slyA +1.3 5.7 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4133 cadC +1.1 10.1 DNA-binding transcriptional activator (NCBI) compare
b3229 sspA +1.0 5.0 stringent starvation protein A (NCBI) compare
b1709 btuD +1.0 1.2 vitamin B12-transporter ATPase (NCBI) compare
b1085 b1085 +1.0 3.4 orf, hypothetical protein (VIMSS) compare
b1831 proQ +1.0 6.1 putative solute/DNA competence effector (NCBI) compare
b3753 rbsR +0.9 7.8 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2659 b2659 +0.9 2.3 orf, hypothetical protein (VIMSS) compare
b3238 yhcN +0.9 2.8 orf, hypothetical protein (VIMSS) compare
b0240 crl +0.8 5.3 DNA-binding transcriptional regulator (NCBI) compare
b4603 rseX +0.8 1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0014 dnaK +0.8 3.3 molecular chaperone DnaK (NCBI) compare
b1150 ymfR +0.8 1.4 e14 prophage; predicted protein (NCBI) compare
b2189 proL +0.7 2.6 tRNA-Pro (NCBI) compare
b1247 oppF +0.7 4.4 oligopeptide transporter subunit (NCBI) compare
b1824 yobF +0.7 2.6 hypothetical protein (NCBI) compare
b2829 ptsP +0.7 6.5 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3516 gadX +0.7 6.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0357 yaiN +0.7 2.2 putative alpha helix chain (VIMSS) compare
b1233 ychJ +0.7 2.3 hypothetical protein (NCBI) compare
b3672 ivbL +0.7 2.0 ilvB operon leader peptide (NCBI) compare
b3206 npr +0.7 3.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1001 yccE +0.7 0.4 hypothetical protein (NCBI) compare
b3146 yraL +0.7 4.9 predicted methyltransferase (NCBI) compare
b3443 yrhA +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b1582 ynfA +0.7 2.7 hypothetical protein (NCBI) compare
b1344 ydaO +0.7 2.5 predicted C32 tRNA thiolase (NCBI) compare
b2502 ppx +0.6 5.1 exopolyphosphatase (NCBI) compare
b3755 yieP +0.6 3.8 predicted transcriptional regulator (NCBI) compare
b1246 oppD +0.6 3.9 oligopeptide transporter ATP-binding component (NCBI) compare
b4012 yjaB +0.6 1.4 predicted acetyltransferase (NCBI) compare
b3027 ygiZ +0.6 0.5 conserved inner membrane protein (NCBI) compare
b3495 uspA +0.6 5.0 universal stress global response regulator (NCBI) compare
b3397 nudE +0.6 4.9 ADP-ribose diphosphatase (NCBI) compare
b1658 purR +0.6 4.5 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1243 oppA +0.6 4.4 oligopeptide transporter subunit (NCBI) compare
b4071 nrfB +0.6 1.5 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b3649 rpoZ +0.6 2.0 DNA-directed RNA polymerase subunit omega (NCBI) compare
b2668 ygaP +0.6 3.6 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1160 elbA +0.6 0.9 hypothetical protein (NCBI) compare
b0660 ybeZ +0.5 3.5 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b0705 ybfL +0.5 0.6 putative receptor protein (VIMSS) compare
b4406 yaeP +0.5 2.4 hypothetical protein (NCBI) compare
b1279 yciS +0.5 1.2 conserved inner membrane protein (NCBI) compare
b0388 aroL +0.5 3.3 shikimate kinase II (NCBI) compare
b4045 yjbJ +0.5 1.3 predicted stress response protein (NCBI) compare
b2007 yeeX +0.5 1.6 conserved protein (RefSeq) compare
b0613 citG +0.5 2.0 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b2448 yffQ +0.5 1.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b1765 ydjA +0.5 0.7 predicted oxidoreductase (NCBI) compare
b1936 intG +0.5 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0436 tig +0.5 3.2 trigger factor (NCBI) compare
b2742 nlpD +0.5 2.7 predicted outer membrane lipoprotein (NCBI) compare
b4345 mcrC +0.5 0.9 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1685 ydiH +0.5 1.4 predicted protein (RefSeq) compare
b1670 ydhU +0.5 2.2 predicted cytochrome (NCBI) compare
b3429 glgA +0.5 4.2 glycogen synthase (NCBI) compare
b3779 gpp +0.5 4.4 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b3446 yrhB +0.5 2.9 hypothetical protein (NCBI) compare
b2229 b2229 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b4585 sroB +0.5 1.1 Novel sRNA, function unknown (RefSeq) compare
b3912 cpxR +0.5 2.7 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b4407 thiS +0.5 1.9 sulfur carrier protein ThiS (NCBI) compare
b4397 creA +0.5 1.6 hypothetical protein (NCBI) compare
b0456 ybaA +0.5 1.6 hypothetical protein (NCBI) compare
b2083 yegZ +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b4676 yoaK +0.5 0.4 expressed protein (RefSeq) compare
b4128 yjdK +0.5 0.4 hypothetical protein (NCBI) compare
b4066 yjcF +0.5 0.4 hypothetical protein (NCBI) compare
b4426 isrA +0.5 1.8 unknown RNA (NCBI) compare
b3166 truB +0.5 3.8 tRNA pseudouridine synthase B (NCBI) compare
b1164 ycgZ +0.5 1.1 hypothetical protein (NCBI) compare
b0294 ykgK +0.5 2.2 predicted regulator (NCBI) compare
b2743 pcm +0.5 2.3 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0092 ddlB +0.4 2.5 D-alanylalanine synthetase (NCBI) compare
b0661 miaB +0.4 3.8 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3512 GadE +0.4 1.6 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b2944 sprT +0.4 2.2 hypothetical protein (NCBI) compare
b1244 oppB +0.4 3.1 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1245 oppC +0.4 2.9 oligopeptide transporter subunit (NCBI) compare
b1448 yncA +0.4 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1597 asr +0.4 2.2 acid shock protein (VIMSS) compare
b1492 gadC +0.4 3.0 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b2637 yfjT +0.4 1.3 CP4-57 prophage; predicted protein (NCBI) compare
b0535 fimZ +0.4 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2199 ccmC +0.4 2.0 heme exporter subunit (NCBI) compare
b0082 mraW +0.4 2.1 S-adenosyl-methyltransferase (NCBI) compare
b2768 ygcP +0.4 1.1 predicted anti-terminator regulatory protein (NCBI) compare
b0851 nfsA +0.4 1.7 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b2181 yejG +0.4 2.2 hypothetical protein (NCBI) compare
b1743 spy +0.4 1.2 envelope stress induced periplasmic protein (NCBI) compare
b1607 ydgC +0.4 1.3 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1400 paaY +0.4 1.1 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1822 rrmA +0.4 1.5 23S rRNA m1G745 methyltransferase (NCBI) compare
b1789 yeaL +0.4 1.7 conserved inner membrane protein (NCBI) compare
b1167 ymgC +0.4 0.2 hypothetical protein (NCBI) compare
b3153 yhbO +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b3228 sspB +0.4 2.2 ClpXP protease specificity-enhancing factor (NCBI) compare
b0955 ycbZ +0.4 3.5 predicted peptidase (NCBI) compare
b1600 mdtJ +0.4 1.0 multidrug efflux system transporter (NCBI) compare
b2644 yfjY +0.4 1.5 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b3292 zntR +0.4 2.0 zinc-responsive transcriptional regulator (NCBI) compare
b3042 b3042 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4477 dgoA +0.4 1.8 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (NCBI) compare
b3884 yihW +0.4 2.3 putative DEOR-type transcriptional regulator (VIMSS) compare
b0503 ybbB +0.4 1.2 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b2479 gcvR +0.4 2.5 transcriptional regulation of gcv operon (VIMSS) compare
b1865 nudB +0.4 0.7 dATP pyrophosphohydrolase (NCBI) compare
b0717 ybgP +0.4 2.8 predicted assembly protein (NCBI) compare
b1253 yciA +0.4 1.2 predicted hydrolase (NCBI) compare
b3875 ompL +0.4 1.5 predicted outer membrane porin L (NCBI) compare
b2646 ypjF +0.4 1.4 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b3163 nlpI +0.4 1.8 hypothetical protein (NCBI) compare
b0782 moaB +0.4 0.9 molybdopterin biosynthesis protein B (NCBI) compare
b3816 corA +0.4 3.1 magnesium/nickel/cobalt transporter (NCBI) compare
b2960 yggH +0.4 2.6 tRNA(m7G46)-methyltransferase (NCBI) compare
b2165 yeiN +0.4 2.1 hypothetical protein (NCBI) compare
b0858 ybjO +0.4 1.3 predicted inner membrane protein (NCBI) compare
b4642 yoeD +0.4 1.1 no description compare
b1461 ydcE +0.4 1.2 4-oxalocrotonate tautomerase (NCBI) compare
b0617 citD +0.4 0.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2004 yeeU +0.4 1.0 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b3261 fis +0.4 1.0 DNA-binding protein Fis (NCBI) compare
b2058 wcaB +0.4 0.8 predicted acyl transferase (NCBI) compare
b0426 yajQ +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b1013 ycdC +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0018 mokC +0.4 0.9 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1920 fliY +0.4 1.1 cystine transporter subunit (NCBI) compare
b1007 b1007 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0122 yacC +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b3686 ibpB +0.4 1.8 heat shock chaperone (NCBI) compare
b3507 dctR +0.4 3.2 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b4293 fecI +0.4 1.2 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b2326 yfcM +0.4 1.5 hypothetical protein (NCBI) compare
b4504 ykfH +0.4 2.0 hypothetical protein (NCBI) compare
b3032 cpdA +0.4 2.8 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b3432 glgB +0.3 3.1 glycogen branching enzyme (NCBI) compare
b3890 yiiF +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2998 yghW +0.3 1.5 hypothetical protein (NCBI) compare
b1919 yedO +0.3 1.6 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1218 chaC +0.3 1.7 regulatory protein for cation transport (RefSeq) compare
b4571 wbbL +0.3 1.7 no description compare
b2317 dedA +0.3 1.2 conserved inner membrane protein (NCBI) compare
b1970 yedX +0.3 1.6 hypothetical protein (NCBI) compare
b0549 ybcO +0.3 1.0 DLP12 prophage; predicted protein (NCBI) compare
b4214 cysQ +0.3 2.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2099 yegU +0.3 1.6 predicted hydrolase (NCBI) compare
b4435 isrC +0.3 1.1 unknown RNA (NCBI) compare
b1329 b1329 +0.3 2.1 putative transport periplasmic protein (VIMSS) compare
b4215 ytfI +0.3 0.8 hypothetical protein (NCBI) compare
b1079 flgH +0.3 1.3 flagellar L-ring protein precursor H (NCBI) compare
b1573 ydfC +0.3 0.9 Qin prophage; predicted protein (RefSeq) compare
b2934 cmtB +0.3 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1358 ydaT +0.3 0.9 Rac prophage; predicted protein (NCBI) compare
b1134 ymfB +0.3 1.2 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b3889 yiiE +0.3 1.1 predicted transcriptional regulator (RefSeq) compare
b2859 ygeO +0.3 0.9 hypothetical protein (RefSeq) compare
b1295 ymjA +0.3 1.6 hypothetical protein (NCBI) compare
b0382 yaiB +0.3 2.5 hypothetical protein (NCBI) compare
b4094 phnN +0.3 1.5 ribose 1,5-bisphosphokinase (NCBI) compare
b0713 ybgL +0.3 2.3 hypothetical protein (NCBI) compare
b1366 ydaY +0.3 1.4 Rac prophage; predicted protein (RefSeq) compare
b1422 ydcI +0.3 1.6 putative transcriptional regulator LYSR-type (VIMSS) compare
b1103 ycfF +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b2916 argP +0.3 2.1 chromosome replication initiation inhibitor protein (NCBI) compare
b4606 ypfM +0.3 1.4 hypothetical protein (RefSeq) compare
b0423 thiI +0.3 2.2 thiamine biosynthesis protein ThiI (NCBI) compare
b0217 yafT +0.3 1.9 predicted aminopeptidase (NCBI) compare
b2221 atoD +0.3 1.4 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b1890 motA +0.3 2.1 flagellar motor protein MotA (NCBI) compare
b1586 b1586 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b0864 artP +0.3 1.8 arginine transporter subunit (NCBI) compare
b4078 yjcO +0.3 1.5 hypothetical protein (NCBI) compare
b3468 yhhN +0.3 2.7 conserved inner membrane protein (NCBI) compare
b1891 flhC +0.3 2.0 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1674 ydhY +0.3 2.2 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b0623 cspE +0.3 0.8 cold shock protein E (NCBI) compare
b2535 csiE +0.3 2.5 orf, hypothetical protein (VIMSS) compare
b2705 srlD +0.3 2.1 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI) compare
b0502 ylbG +0.3 1.1 no description compare
b3131 agaR +0.3 1.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2145 yeiS +0.3 1.6 predicted inner membrane protein (NCBI) compare
b3002 yqhA +0.3 1.1 hypothetical protein (NCBI) compare
b4097 phnK +0.3 1.0 carbon-phosphorus lyase complex subunit (NCBI) compare
b0696 kdpC +0.3 1.4 potassium-transporting ATPase subunit C (NCBI) compare
b4592 yccB +0.3 1.1 hypothetical protein (RefSeq) compare
b2457 cchA +0.3 1.4 detox protein (VIMSS) compare
b1195 ymgE +0.3 1.7 predicted inner membrane protein (NCBI) compare
b3752 rbsK +0.3 2.3 ribokinase (NCBI) compare
b0191 yaeJ +0.3 1.7 hypothetical protein (NCBI) compare
b2047 wcaJ +0.3 1.6 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b2911 ssrS +0.3 0.4 6S regulatory RNA (NCBI) compare
b3812 yigB +0.3 1.8 predicted hydrolase (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Methotrexate across organisms