Experiment set53IT026 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 1.98 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b1243 oppA +3.7 26.0 oligopeptide transporter subunit (NCBI) compare
b1246 oppD +3.7 31.7 oligopeptide transporter ATP-binding component (NCBI) compare
b1244 oppB +3.6 26.6 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1247 oppF +3.6 24.6 oligopeptide transporter subunit (NCBI) compare
b1245 oppC +3.6 28.8 oligopeptide transporter subunit (NCBI) compare
b2684 mprA +2.7 8.2 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0929 ompF +2.5 16.9 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b3911 cpxA +2.4 19.6 two-component sensor protein (NCBI) compare
b1236 galU +1.8 6.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2469 narQ +1.8 6.4 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b3169 nusA +1.7 3.8 transcription elongation factor NusA (NCBI) compare
b2078 baeS +1.5 8.5 sensory histidine kinase in two-component regulatory system with BaeR (NCBI) compare
b3842 rfaH +1.5 6.9 transcriptional activator RfaH (NCBI) compare
b0688 pgm +1.4 5.7 phosphoglucomutase (NCBI) compare
b0464 acrR +1.3 10.8 DNA-binding transcriptional repressor (NCBI) compare
b4402 yjjY +1.3 2.4 hypothetical protein (NCBI) compare
b3979 thrT +1.2 0.9 tRNA-Thr (NCBI) compare
b1530 marR +1.2 2.8 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3631 rfaG +1.2 10.0 glucosyltransferase I (NCBI) compare
b1936 intG +1.2 1.8 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2911 ssrS +1.2 2.2 6S regulatory RNA (NCBI) compare
b0178 hlpA +1.2 6.7 periplasmic chaperone (NCBI) compare
b2303 folX +1.1 10.0 D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI) compare
b1613 manA +1.1 2.7 mannose-6-phosphate isomerase (VIMSS) compare
b4603 rseX +1.0 1.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1606 folM +1.0 6.7 short chain dehydrogenase (NCBI) compare
b3181 greA +1.0 5.5 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2617 smpA +1.0 4.4 small membrane protein A (VIMSS) compare
b4246 pyrL +1.0 1.9 pyrBI operon leader peptide (NCBI) compare
b3341 rpsG +0.9 2.1 30S ribosomal protein S7 (NCBI) compare
b3778 rep +0.9 6.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1150 ymfR +0.9 1.7 e14 prophage; predicted protein (NCBI) compare
b4041 plsB +0.9 3.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4163 glyV +0.8 2.2 tRNA-Gly (NCBI) compare
b1284 yciT +0.8 5.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1482 osmC +0.8 2.5 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b1818 manY +0.7 2.0 mannose-specific enzyme IIC component of PTS (NCBI) compare
b2659 b2659 +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b4279 yjhB +0.7 0.5 putative transport protein (VIMSS) compare
b2217 rcsB +0.7 5.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3180 yhbY +0.7 4.2 predicted RNA-binding protein (NCBI) compare
b0730 mngR +0.7 1.6 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1257 yciE +0.7 0.4 hypothetical protein (NCBI) compare
b0759 galE +0.6 4.6 UDP-galactose-4-epimerase (VIMSS) compare
b2531 iscR +0.6 1.6 DNA-binding transcriptional repressor (NCBI) compare
b3423 glpR +0.6 5.5 DNA-binding transcriptional repressor (NCBI) compare
b0209 yafD +0.6 4.2 hypothetical protein (NCBI) compare
b2821 ptr +0.6 5.6 protease III (NCBI) compare
b4066 yjcF +0.6 0.5 hypothetical protein (NCBI) compare
b3443 yrhA +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b1095 fabF +0.6 4.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b4030 yjbA +0.6 4.1 phosphate-starvation-inducible protein PsiE (NCBI) compare
b3512 GadE +0.6 2.8 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0122 yacC +0.6 4.1 orf, hypothetical protein (VIMSS) compare
b2903 gcvP +0.6 5.3 glycine dehydrogenase (NCBI) compare
b3082 ygjM +0.6 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1837 yebW +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b3095 yqjA +0.6 4.7 conserved inner membrane protein (NCBI) compare
b3620 rfaF +0.6 2.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b4648 ysaC +0.6 2.0 no description compare
b1164 ycgZ +0.6 1.5 hypothetical protein (NCBI) compare
b4391 yjjK +0.6 4.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2642 yfjW +0.6 1.0 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3627 rfaI +0.6 4.4 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1831 proQ +0.6 3.0 putative solute/DNA competence effector (NCBI) compare
b1242 ychE +0.5 2.5 predicted inner membrane protein (NCBI) compare
b3933 ftsN +0.5 4.4 essential cell division protein (NCBI) compare
b3630 rfaP +0.5 3.8 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2904 gcvH +0.5 1.9 glycine cleavage system protein H (NCBI) compare
b0263 afuB +0.5 2.4 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b3026 qseC +0.5 1.2 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1361 ydaW +0.5 0.5 orf, hypothetical protein (VIMSS) compare
b2450 yffS +0.5 1.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b1159 mcrA +0.5 1.0 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b0046 kefF +0.5 3.4 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b2934 cmtB +0.5 0.6 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1842 holE +0.5 1.7 DNA polymerase III, theta subunit (NCBI) compare
b3628 rfaB +0.5 4.0 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1961 dcm +0.5 1.3 DNA cytosine methylase (NCBI) compare
b1583 ynfB +0.5 1.9 hypothetical protein (NCBI) compare
b0401 brnQ +0.5 3.8 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b2218 rcsC +0.5 4.0 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b1548 nohA +0.5 1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b3194 yrbE +0.5 3.8 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1919 yedO +0.4 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b3146 yraL +0.4 3.4 predicted methyltransferase (NCBI) compare
b1085 b1085 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b4570 lomR +0.4 1.5 no description compare
b2012 yeeD +0.4 2.0 hypothetical protein (NCBI) compare
b3238 yhcN +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b3500 gor +0.4 1.1 glutathione reductase (NCBI) compare
b2181 yejG +0.4 2.1 hypothetical protein (NCBI) compare
b1070 flgN +0.4 1.1 export chaperone for FlgK and FlgL (NCBI) compare
b4651 yibW +0.4 0.3 no description compare
b1434 ydcN +0.4 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3429 glgA +0.4 3.4 glycogen synthase (NCBI) compare
b2749 ygbE +0.4 2.7 conserved inner membrane protein (NCBI) compare
b1804 rnd +0.4 2.2 ribonuclease D (NCBI) compare
b1111 ycfQ +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1674 ydhY +0.4 2.7 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b3391 hofQ +0.4 3.4 predicted fimbrial transporter (NCBI) compare
b2236 yfaE +0.4 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4655 ythA +0.4 0.7 expressed protein (RefSeq) compare
b4436 ryeC +0.4 1.4 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1349 recT +0.4 2.7 Rac prophage; recombination and repair protein (NCBI) compare
b4045 yjbJ +0.4 1.5 predicted stress response protein (NCBI) compare
b3766 ilvL +0.4 2.0 ilvG operon leader peptide (NCBI) compare
b2480 bcp +0.4 2.9 thioredoxin-dependent thiol peroxidase (NCBI) compare
b2477 nlpB +0.4 2.0 lipoprotein-34 (VIMSS) compare
b1642 slyA +0.4 1.5 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4173 hflX +0.4 2.7 predicted GTPase (NCBI) compare
b2168 fruK +0.4 0.6 1-phosphofructokinase (NCBI) compare
b3192 yrbC +0.4 3.2 predicted ABC-type organic solvent transporter (NCBI) compare
b1949 fliQ +0.4 1.5 flagellar biosynthesis protein Q (NCBI) compare
b1604 ydgH +0.4 2.5 hypothetical protein (NCBI) compare
b4596 yciZ +0.4 1.0 hypothetical protein (RefSeq) compare
b1527 yneK +0.4 1.3 hypothetical protein (NCBI) compare
b1553 ydfP +0.4 1.3 Qin prophage; conserved protein (NCBI) compare
b2029 gnd +0.4 3.0 6-phosphogluconate dehydrogenase (NCBI) compare
b1344 ydaO +0.4 1.2 predicted C32 tRNA thiolase (NCBI) compare
b2569 lepA +0.4 1.7 GTP-binding protein LepA (NCBI) compare
b3858 yihD +0.4 1.9 hypothetical protein (NCBI) compare
b0213 yafS +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b2346 vacJ +0.4 2.8 predicted lipoprotein (NCBI) compare
b1536 ydeI +0.4 0.6 hypothetical protein (NCBI) compare
b3397 nudE +0.4 2.7 ADP-ribose diphosphatase (NCBI) compare
b2156 lysP +0.4 3.1 lysine transporter (NCBI) compare
b4579 yaiX +0.4 2.4 no description compare
b0955 ycbZ +0.4 2.9 predicted peptidase (NCBI) compare
b3191 yrbB +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b1001 yccE +0.4 0.2 hypothetical protein (NCBI) compare
b0047 kefC +0.4 3.0 glutathione-regulated potassium-efflux system protein (NCBI) compare
b1492 gadC +0.4 2.4 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b3213 gltD +0.4 3.0 glutamate synthase, 4Fe-4S protein, small subunit (NCBI) compare
b1773 ydjI +0.4 1.2 predicted aldolase (NCBI) compare
b3507 dctR +0.4 3.2 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b1761 gdhA +0.4 2.4 glutamate dehydrogenase (NCBI) compare
b1630 rsxD +0.4 1.7 electron transport complex protein RnfD (NCBI) compare
b0949 uup +0.4 2.7 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1582 ynfA +0.3 1.7 hypothetical protein (NCBI) compare
b0065 yabI +0.3 2.1 conserved inner membrane protein (NCBI) compare
b4686 yshB +0.3 2.1 expressed protein (RefSeq) compare
b4554 yibT +0.3 1.8 hypothetical protein (NCBI) compare
b2449 yffR +0.3 1.8 CPZ-55 prophage; predicted protein (NCBI) compare
b1476 fdnI +0.3 0.6 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b4679 yohP +0.3 1.5 expressed protein (RefSeq) compare
b0920 ycbC +0.3 2.1 conserved inner membrane protein (NCBI) compare
b2812 ygdL +0.3 2.8 hypothetical protein (NCBI) compare
b1524 yneH +0.3 0.6 predicted glutaminase (NCBI) compare
b3466 yhhL +0.3 2.0 conserved inner membrane protein (NCBI) compare
b3133 agaV +0.3 0.9 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b0357 yaiN +0.3 1.0 putative alpha helix chain (VIMSS) compare
b1579 intQ +0.3 1.5 Qin prophage; predicted defective integrase (RefSeq) compare
b3212 gltB +0.3 3.2 glutamate synthase, large subunit (NCBI) compare
b2037 rfbX +0.3 2.6 predicted polisoprenol-linked O-antigen transporter (NCBI) compare
b1363 trkG +0.3 0.3 Rac prophage; potassium transporter subunit (NCBI) compare
b0885 aat +0.3 1.8 leucyl/phenylalanyl-tRNA--protein transferase (NCBI) compare
b1539 ydfG +0.3 1.5 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b1441 ydcT +0.3 1.9 predicted spermidine/putrescine transporter subunit (NCBI) compare
b2570 rseC +0.3 2.4 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b2369 evgA +0.3 1.5 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b4511 ybdZ +0.3 1.6 hypothetical protein (NCBI) compare
b3153 yhbO +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b3740 gidB +0.3 2.6 glucose-inhibited division protein B (NCBI) compare
b4692 oweE +0.3 1.1 no description compare
b0125 hpt +0.3 1.7 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b2528 iscA +0.3 0.8 iron-sulfur cluster assembly protein (NCBI) compare
b1907 tyrP +0.3 1.8 tyrosine transporter (NCBI) compare
b2681 ygaY +0.3 2.2 predicted transporter (pseudogene) (RefSeq) compare
b0946 ycbW +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2960 yggH +0.3 2.1 tRNA(m7G46)-methyltransferase (NCBI) compare
b2614 grpE +0.3 1.3 heat shock protein (NCBI) compare
b1195 ymgE +0.3 1.6 predicted inner membrane protein (NCBI) compare
b1905 ftn +0.3 2.5 ferritin iron storage protein (cytoplasmic) (NCBI) compare
b3555 yiaG +0.3 1.3 predicted transcriptional regulator (NCBI) compare
b3193 yrbD +0.3 2.4 predicted ABC-type organic solvent transporter (NCBI) compare
b1125 potB +0.3 1.5 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2006 yeeW +0.3 2.0 CP4-44 prophage; predicted protein (RefSeq) compare
b0880 cspD +0.3 1.3 cold shock protein homolog (NCBI) compare
b3472 b3472 +0.3 2.3 orf, hypothetical protein (VIMSS) compare
b2488 hyfH +0.3 1.5 hydrogenase 4, Fe-S subunit (NCBI) compare
b3775 ppiC +0.3 1.9 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b3995 rsd +0.3 1.7 stationary phase protein, binds sigma 70 RNA polymerase subunit (NCBI) compare
b0775 bioB +0.3 2.1 biotin synthase (NCBI) compare
b0736 ybgC +0.3 1.5 predicted acyl-CoA thioesterase (NCBI) compare
b1038 csgF +0.3 0.9 predicted transport protein (NCBI) compare
b3794 rffM +0.3 1.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4227 ytfQ +0.3 1.9 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b4557 yidD +0.3 1.4 hypothetical protein (NCBI) compare
b1090 plsX +0.3 1.2 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2204 napH +0.3 1.2 quinol dehydrogenase membrane component (NCBI) compare
b3632 rfaQ +0.3 2.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1954 dsrA +0.3 1.3 regulatory antisense RNA (NCBI) compare
b2259 pmrD +0.3 0.7 polymyxin resistance protein B (VIMSS) compare
b3120 yhaB +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b0487 cueR +0.3 1.7 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b4012 yjaB +0.3 1.7 predicted acetyltransferase (NCBI) compare
b1007 b1007 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b0232 yafN +0.3 0.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0798 ybiA +0.3 1.1 hypothetical protein (NCBI) compare


Specific Phenotypes

For 13 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms