Experiment set53IT025 for Escherichia coli BW25113

Compare to:

M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr

200 most important genes:

  gene name fitness t score description  
b2838 lysA -7.9 -9.4 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b2476 purC -6.7 -4.6 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b0907 serC -6.7 -12.0 phosphoserine aminotransferase (NCBI) compare
b0004 thrC -6.4 -14.9 threonine synthase (NCBI) compare
b4388 serB -6.4 -11.5 3-phosphoserine phosphatase (NCBI) compare
b3960 argH -6.1 -21.7 argininosuccinate lyase (NCBI) compare
b3172 argG -6.1 -20.7 argininosuccinate synthase (NCBI) compare
b1261 trpB -6.0 -6.0 tryptophan synthase subunit beta (NCBI) compare
b0522 purK -5.8 -8.0 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b2499 purM -5.8 -4.0 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2557 purL -5.8 -6.9 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2025 hisF -5.7 -8.7 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b1260 trpA -5.6 -9.5 tryptophan synthase subunit alpha (NCBI) compare
b2913 serA -5.6 -15.2 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b4006 purH -5.5 -11.2 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b2312 purF -5.4 -6.4 amidophosphoribosyltransferase (NCBI) compare
b4005 purD -5.4 -10.3 phosphoribosylamine--glycine ligase (NCBI) compare
b0003 thrB -5.3 -9.0 homoserine kinase (NCBI) compare
b2329 aroC -5.0 -9.6 chorismate synthase (NCBI) compare
b3956 ppc -4.9 -27.5 phosphoenolpyruvate carboxylase (NCBI) compare
b2320 pdxB -4.9 -12.1 erythronate-4-phosphate dehydrogenase (NCBI) compare
b3281 aroE -4.9 -19.3 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2564 pdxJ -4.8 -9.6 pyridoxal phosphate biosynthetic protein (NCBI) compare
b3389 aroB -4.7 -13.3 3-dehydroquinate synthase (NCBI) compare
b0002 thrA -4.6 -19.9 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b2762 cysH -4.6 -14.4 phosphoadenosine phosphosulfate reductase (NCBI) compare
b0908 aroA -4.6 -14.6 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0052 pdxA -4.6 -18.7 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b3941 metF -4.5 -18.9 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b0523 purE -4.3 -4.4 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4214 cysQ -4.2 -18.5 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2022 hisB -4.1 -11.7 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b2750 cysC -4.1 -14.8 adenylylsulfate kinase (NCBI) compare
b2023 hisH -4.0 -11.0 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b2024 hisA -4.0 -5.2 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b0726 sucA -4.0 -7.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b3828 metR -4.0 -20.6 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b3829 metE -4.0 -24.2 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b0889 lrp -3.9 -4.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2020 hisD -3.9 -15.0 histidinol dehydrogenase (NCBI) compare
b2021 hisC -3.9 -13.6 histidinol-phosphate aminotransferase (NCBI) compare
b1185 dsbB -3.8 -3.7 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0242 proB -3.8 -11.1 gamma-glutamyl kinase (NCBI) compare
b1693 aroD -3.8 -8.0 3-dehydroquinate dehydratase (NCBI) compare
b3774 ilvC -3.8 -25.4 ketol-acid reductoisomerase (NCBI) compare
b1062 pyrC -3.8 -2.6 dihydroorotase (NCBI) compare
b2600 tyrA -3.7 -8.2 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b3771 ilvD -3.7 -23.5 dihydroxy-acid dehydratase (NCBI) compare
b3860 dsbA -3.7 -7.1 periplasmic protein disulfide isomerase I (NCBI) compare
b2764 cysJ -3.5 -14.2 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b2501 ppk -3.5 -15.0 polyphosphate kinase (NCBI) compare
b0145 dksA -3.5 -2.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0243 proA -3.4 -15.1 gamma-glutamyl phosphate reductase (NCBI) compare
b3770 ilvE -3.4 -18.0 branched-chain amino acid aminotransferase (NCBI) compare
b0142 folK -3.3 -1.4 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2763 cysI -3.3 -11.2 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b0072 leuC -3.2 -20.2 isopropylmalate isomerase large subunit (NCBI) compare
b0071 leuD -3.2 -15.3 isopropylmalate isomerase small subunit (NCBI) compare
b0073 leuB -3.2 -14.1 3-isopropylmalate dehydrogenase (VIMSS) compare
b2839 lysR -3.1 -4.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2479 gcvR -3.1 -9.3 transcriptional regulation of gcv operon (VIMSS) compare
b3870 glnA -3.0 -2.0 glutamine synthetase (NCBI) compare
b2019 hisG -3.0 -9.1 ATP phosphoribosyltransferase (NCBI) compare
b4702 mgtL -2.9 -2.0 regulatory leader peptide for mgtA (NCBI) compare
b0074 leuA -2.9 -17.7 2-isopropylmalate synthase (NCBI) compare
b4242 mgtA -2.9 -8.5 magnesium transporter (NCBI) compare
b0222 lpcA -2.9 -1.9 phosphoheptose isomerase (NCBI) compare
b0720 gltA -2.8 -11.4 citrate synthase (NCBI) compare
b3164 pnp -2.8 -5.3 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3008 metC -2.8 -18.3 cystathionine beta-lyase (NCBI) compare
b3368 cysG -2.8 -21.7 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b3642 pyrE -2.8 -1.9 orotate phosphoribosyltransferase (NCBI) compare
b1235 rssB -2.8 -7.8 response regulator of RpoS (NCBI) compare
b3781 trxA -2.7 -8.6 thioredoxin 1 (VIMSS) compare
b1262 trpC -2.7 -5.3 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b2500 purN -2.6 -9.8 phosphoribosylglycinamide formyltransferase (NCBI) compare
b0585 fes -2.6 -11.5 enterobactin/ferric enterobactin esterase (NCBI) compare
b2026 hisI -2.6 -10.7 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b3204 ptsN -2.6 -7.6 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3957 argE -2.5 -19.3 acetylornithine deacetylase (NCBI) compare
b0945 pyrD -2.5 -1.7 dihydroorotate dehydrogenase (NCBI) compare
b4245 pyrB -2.5 -1.7 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0588 fepC -2.4 -13.8 iron-enterobactin transporter subunit (NCBI) compare
b0032 carA -2.3 -2.8 carbamoyl-phosphate synthase small subunit (NCBI) compare
b1290 sapF -2.3 -2.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0149 mrcB -2.3 -12.7 penicillin-binding protein 1b (NCBI) compare
b4451 ryhB -2.3 -3.6 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0589 fepG -2.3 -11.3 iron-enterobactin transporter subunit (NCBI) compare
b1677 lpp -2.3 -3.7 murein lipoprotein (NCBI) compare
b1281 pyrF -2.3 -3.0 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0590 fepD -2.3 -12.9 iron-enterobactin transporter subunit (NCBI) compare
b2414 cysK -2.3 -12.9 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b2599 pheA -2.3 -7.8 fused chorismate mutase P/prephenate dehydratase (NCBI) compare
b0438 clpX -2.3 -2.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0386 proC -2.2 -3.0 pyrroline-5-carboxylate reductase (NCBI) compare
b3959 argB -2.2 -16.2 acetylglutamate kinase (RefSeq) compare
b3052 rfaE -2.2 -2.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0592 fepB -2.2 -14.7 iron-enterobactin transporter subunit (NCBI) compare
b0439 lon -2.2 -4.3 DNA-binding ATP-dependent protease La (NCBI) compare
b3958 argC -2.2 -15.2 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b1263 trpD -2.2 -4.3 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0928 aspC -2.1 -10.9 aspartate aminotransferase, PLP-dependent (NCBI) compare
b2818 argA -2.1 -9.8 N-acetylglutamate synthase (NCBI) compare
b1252 tonB -2.1 -6.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0632 dacA -2.1 -10.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2188 yejM -2.0 -11.2 predicted hydrolase, inner membrane (NCBI) compare
b4171 miaA -2.0 -4.3 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0564 appY -2.0 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3006 exbB -2.0 -11.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3849 trkH -1.9 -13.3 potassium transporter (NCBI) compare
b3005 exbD -1.9 -8.7 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1264 trpE -1.9 -5.0 anthranilate synthase component I (NCBI) compare
b3169 nusA -1.9 -2.5 transcription elongation factor NusA (NCBI) compare
b2318 truA -1.9 -4.1 tRNA pseudouridine synthase A (NCBI) compare
b3772 ilvA -1.9 -15.4 threonine dehydratase (NCBI) compare
b3390 aroK -1.8 -2.6 shikimate kinase I (NCBI) compare
b2340 sixA -1.8 -6.2 phosphohistidine phosphatase (NCBI) compare
b0688 pgm -1.8 -3.9 phosphoglucomutase (NCBI) compare
b3650 spoT -1.8 -9.9 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b0584 fepA -1.7 -13.8 iron-enterobactin outer membrane transporter (NCBI) compare
b2752 cysD -1.7 -7.7 sulfate adenylyltransferase subunit 2 (NCBI) compare
b3993 thiE -1.7 -8.9 thiamine-phosphate pyrophosphorylase (NCBI) compare
b0033 carB -1.7 -2.5 carbamoyl-phosphate synthase large subunit (NCBI) compare
b0888 trxB -1.6 -4.5 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b0437 clpP -1.6 -1.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3609 secB -1.6 -3.4 export protein SecB (NCBI) compare
b0049 apaH -1.6 -1.1 diadenosinetetraphosphatase (NCBI) compare
b2601 aroF -1.6 -7.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tyrosine-repressible (NCBI) compare
b3924 fpr -1.6 -10.6 ferredoxin-NADP reductase (NCBI) compare
b3916 pfkA -1.6 -4.0 6-phosphofructokinase (NCBI) compare
b3493 pitA -1.6 -7.9 phosphate transporter, low-affinity (NCBI) compare
b0683 fur -1.6 -1.9 ferric uptake regulator (NCBI) compare
b1855 lpxM -1.6 -6.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3621 rfaC -1.5 -1.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3527 yhjJ -1.5 -13.9 predicted zinc-dependent peptidase (NCBI) compare
b3620 rfaF -1.5 -3.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2313 cvpA -1.5 -1.9 membrane protein required for colicin V production (NCBI) compare
b1273 yciN -1.5 -2.7 hypothetical protein (NCBI) compare
b0630 lipB -1.5 -1.7 protein of lipoate biosynthesis (VIMSS) compare
b3183 obgE -1.5 -2.1 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3994 thiC -1.5 -12.7 thiamine biosynthesis protein ThiC (NCBI) compare
b0200 gmhB -1.4 -1.0 hypothetical protein (NCBI) compare
b2103 thiD -1.4 -9.6 phosphomethylpyrimidine kinase (NCBI) compare
b1084 rne -1.4 -5.2 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3939 metB -1.4 -9.3 cystathionine gamma-synthase (NCBI) compare
b1684 sufA -1.4 -3.8 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b4481 rffT -1.4 -3.6 4-alpha-L-fucosyltransferase (NCBI) compare
b3630 rfaP -1.3 -6.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0750 nadA -1.3 -8.2 quinolinate synthetase (NCBI) compare
b3792 wzxE -1.3 -2.7 O-antigen translocase (NCBI) compare
b0926 ycbK -1.3 -7.0 hypothetical protein (NCBI) compare
b0767 ybhE -1.3 -5.1 6-phosphogluconolactonase (NCBI) compare
b4025 pgi -1.3 -8.6 glucose-6-phosphate isomerase (NCBI) compare
b3398 yrfF -1.3 -3.9 predicted inner membrane protein (NCBI) compare
b0078 ilvH -1.3 -9.0 acetolactate synthase small subunit (NCBI) compare
b0075 leuL -1.3 -3.8 leu operon leader peptide (NCBI) compare
b2751 cysN -1.3 -6.2 sulfate adenylyltransferase subunit 1 (NCBI) compare
b0113 pdhR -1.2 -4.8 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b0077 ilvI -1.2 -9.7 acetolactate synthase III large subunit (NCBI) compare
b2175 spr -1.2 -3.4 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2611 ypjD -1.2 -8.5 predicted inner membrane protein (RefSeq) compare
b3791 rffA -1.2 -4.1 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1291 sapD -1.2 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3790 rffC -1.2 -2.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b1882 cheY -1.2 -1.0 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3636 rpmG -1.2 -3.0 50S ribosomal protein L33 (NCBI) compare
b3247 cafA -1.2 -9.5 bundles of cytoplasmic filaments (VIMSS) compare
b0741 pal -1.1 -1.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0080 fruR -1.1 -5.7 DNA-binding transcriptional dual regulator (NCBI) compare
b2689 yqaA -1.1 -2.6 conserved inner membrane protein (NCBI) compare
b0088 murD -1.1 -3.2 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2912 ygfA -1.1 -2.2 putative ligase (VIMSS) compare
b3838 tatB -1.1 -5.2 sec-independent translocase (NCBI) compare
b1176 minC -1.1 -3.1 septum formation inhibitor (NCBI) compare
b4178 yjeB -1.1 -4.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1951 rcsA -1.1 -1.5 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b2808 gcvA -1.1 -3.7 DNA-binding transcriptional dual regulator (NCBI) compare
b1686 ydiI -1.1 -3.5 hypothetical protein (NCBI) compare
b1292 sapC -1.1 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3990 thiH -1.0 -6.8 thiamine biosynthesis protein ThiH (NCBI) compare
b0400 phoR -1.0 -8.3 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b3631 rfaG -1.0 -7.1 glucosyltransferase I (NCBI) compare
b2282 nuoH -1.0 -3.6 NADH dehydrogenase subunit H (NCBI) compare
b3935 priA -1.0 -3.4 primosome assembly protein PriA (NCBI) compare
b1865 nudB -1.0 -1.6 dATP pyrophosphohydrolase (NCBI) compare
b1850 eda -1.0 -1.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1111 ycfQ -1.0 -2.6 orf, hypothetical protein (VIMSS) compare
b0912 ihfB -1.0 -0.6 integration host factor subunit beta (NCBI) compare
b3375 yhfR -1.0 -7.2 putative transcriptional regulator (VIMSS) compare
b1192 ldcA -1.0 -2.4 L,D-carboxypeptidase A (NCBI) compare
b4179 vacB -1.0 -4.1 putative enzyme (VIMSS) compare
b3649 rpoZ -1.0 -2.9 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0957 ompA -1.0 -3.2 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3787 rffD -1.0 -6.4 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2683 ygaH -1.0 -3.3 predicted inner membrane protein (NCBI) compare
b3236 mdh -1.0 -5.4 malate dehydrogenase (NCBI) compare
b1286 rnb -1.0 -2.1 exoribonuclease II (NCBI) compare
b4013 metA -0.9 -5.1 homoserine O-succinyltransferase (NCBI) compare
b3786 rffE -0.9 -6.5 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2620 smpB -0.9 -1.1 SsrA-binding protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For no stress control Dimethyl Sulfoxide in Escherichia coli BW25113

For no stress control Dimethyl Sulfoxide across organisms