Experiment set53IT016 for Escherichia coli BW25113
M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Quality Metrics:
Time0 | 8/11/21 Keio_ML9_set53 | which Time0s the sample was compared to |
cor12 | 0.37 | rank correlation(fit1, fit2), where fit1 is fitness for the first half (10-50%) and fit2 is fitness for the second half (50-90%) of each gene |
maxFit | 2.85 | The maximum fitness value |
opcor | 0.19 | rank correlation(upstream gene, downstream gene) over pairs that are adjacent and likely to be in the same operon |
adjcor | 0.02 | like opcor but for adjacent genes that are not on the same strand |
gccor | -0.10 | linear correlation of gene fitness and gene GC content |
mad12 | 0.14 | median absolute difference of fit1, fit2 |
mad12c | 0.72 | median absolute difference of log count for 1st and 2nd half of genes in this sample |
mad12c_t0 | 0.70 | like mad12c but for the Time0s |
gMed | 463 | median reads per gene in this sample |
gMedt0 | 1075 | median reads per gene in the Time0 sample |
gMean | 693 | mean reads per gene in this sample |
nMapped | 3.843 M | #reads for this sample that corresponded to a known strain (in millions) |
nPastEnd | 0.000 M | #reads that corresponded to a strain that has an insertion within the suicide vector instead of within the genome. |
nGenic | 2.627 M | #reads that lie within central 10-90% of a gene |
nUsed | 2.624 M | #reads used to estimate gene fitness (genic and enough coverage for strain and for gene) |
Specific Phenotypes
For 3 genes in this experiment
For no stress control Dimethyl Sulfoxide in Escherichia coli BW25113