Experiment set53IT016 for Escherichia coli BW25113

Compare to:

M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

200 most important genes:

  gene name fitness t score description  
b2838 lysA -6.5 -16.2 diaminopimelate decarboxylase, PLP-binding (NCBI) compare
b1261 trpB -5.2 -5.3 tryptophan synthase subunit beta (NCBI) compare
b4388 serB -5.1 -12.8 3-phosphoserine phosphatase (NCBI) compare
b0907 serC -5.1 -13.4 phosphoserine aminotransferase (NCBI) compare
b0522 purK -4.6 -11.0 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b2476 purC -4.5 -3.6 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1260 trpA -4.4 -8.4 tryptophan synthase subunit alpha (NCBI) compare
b2188 yejM -4.4 -15.0 predicted hydrolase, inner membrane (NCBI) compare
b2557 purL -4.4 -13.9 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2913 serA -4.2 -14.7 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b0004 thrC -4.2 -15.6 threonine synthase (NCBI) compare
b2312 purF -4.1 -7.8 amidophosphoribosyltransferase (NCBI) compare
b2499 purM -4.1 -6.8 phosphoribosylaminoimidazole synthetase (NCBI) compare
b4006 purH -4.1 -13.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4005 purD -4.0 -12.8 phosphoribosylamine--glycine ligase (NCBI) compare
b0523 purE -3.9 -5.1 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b1882 cheY -3.8 -1.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b0003 thrB -3.7 -9.0 homoserine kinase (NCBI) compare
b1281 pyrF -3.5 -2.4 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0957 ompA -3.5 -7.1 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b3281 aroE -3.4 -18.9 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3389 aroB -3.3 -13.7 3-dehydroquinate synthase (NCBI) compare
b2320 pdxB -3.3 -10.9 erythronate-4-phosphate dehydrogenase (NCBI) compare
b2564 pdxJ -3.2 -10.2 pyridoxal phosphate biosynthetic protein (NCBI) compare
b3960 argH -3.1 -14.0 argininosuccinate lyase (NCBI) compare
b0142 folK -3.1 -1.3 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1185 dsbB -3.1 -4.1 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0145 dksA -3.1 -3.6 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0052 pdxA -3.0 -14.3 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b3860 dsbA -3.0 -8.1 periplasmic protein disulfide isomerase I (NCBI) compare
b3390 aroK -2.8 -2.7 shikimate kinase I (NCBI) compare
b3941 metF -2.8 -18.7 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b0632 dacA -2.8 -9.3 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3977 tyrU -2.7 -1.6 tRNA-Tyr (NCBI) compare
b1693 aroD -2.7 -7.9 3-dehydroquinate dehydratase (NCBI) compare
b0908 aroA -2.7 -13.7 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0222 lpcA -2.7 -1.8 phosphoheptose isomerase (NCBI) compare
b3642 pyrE -2.6 -1.8 orotate phosphoribosyltransferase (NCBI) compare
b0002 thrA -2.6 -15.9 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b3172 argG -2.5 -15.3 argininosuccinate synthase (NCBI) compare
b3829 metE -2.4 -18.0 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (NCBI) compare
b0726 sucA -2.4 -6.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b1062 pyrC -2.4 -5.7 dihydroorotase (NCBI) compare
b2329 aroC -2.2 -8.0 chorismate synthase (NCBI) compare
b3956 ppc -2.2 -17.6 phosphoenolpyruvate carboxylase (NCBI) compare
b4245 pyrB -2.2 -2.1 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1235 rssB -2.2 -7.3 response regulator of RpoS (NCBI) compare
b3828 metR -2.1 -13.3 DNA-binding transcriptional activator, homocysteine-binding (NCBI) compare
b4242 mgtA -2.1 -9.2 magnesium transporter (NCBI) compare
b2024 hisA -2.1 -5.4 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b0438 clpX -2.1 -2.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2501 ppk -2.0 -10.3 polyphosphate kinase (NCBI) compare
b3052 rfaE -2.0 -2.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2839 lysR -1.9 -5.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2762 cysH -1.9 -9.4 phosphoadenosine phosphosulfate reductase (NCBI) compare
b0945 pyrD -1.9 -4.0 dihydroorotate dehydrogenase (NCBI) compare
b0149 mrcB -1.9 -10.7 penicillin-binding protein 1b (NCBI) compare
b2600 tyrA -1.9 -9.6 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b0032 carA -1.9 -3.8 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0564 appY -1.8 -1.0 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b2750 cysC -1.8 -10.9 adenylylsulfate kinase (NCBI) compare
b2912 ygfA -1.8 -2.7 putative ligase (VIMSS) compare
b1262 trpC -1.8 -4.9 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b4025 pgi -1.8 -9.9 glucose-6-phosphate isomerase (NCBI) compare
b0073 leuB -1.8 -10.6 3-isopropylmalate dehydrogenase (VIMSS) compare
b0889 lrp -1.7 -3.8 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3916 pfkA -1.7 -3.5 6-phosphofructokinase (NCBI) compare
b2569 lepA -1.7 -4.4 GTP-binding protein LepA (NCBI) compare
b1290 sapF -1.7 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0386 proC -1.7 -3.0 pyrroline-5-carboxylate reductase (NCBI) compare
b2023 hisH -1.7 -7.0 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3771 ilvD -1.7 -13.9 dihydroxy-acid dehydratase (NCBI) compare
b0072 leuC -1.6 -13.0 isopropylmalate isomerase large subunit (NCBI) compare
b2022 hisB -1.6 -8.5 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b2764 cysJ -1.6 -7.3 sulfite reductase, alpha subunit, flavoprotein (NCBI) compare
b2025 hisF -1.6 -4.5 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b3774 ilvC -1.6 -13.3 ketol-acid reductoisomerase (NCBI) compare
b0720 gltA -1.5 -8.1 citrate synthase (NCBI) compare
b0437 clpP -1.5 -1.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3870 glnA -1.5 -3.4 glutamine synthetase (NCBI) compare
b0928 aspC -1.5 -7.0 aspartate aminotransferase, PLP-dependent (NCBI) compare
b2763 cysI -1.5 -6.7 sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI) compare
b0243 proA -1.5 -8.6 gamma-glutamyl phosphate reductase (NCBI) compare
b1291 sapD -1.5 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2021 hisC -1.5 -6.0 histidinol-phosphate aminotransferase (NCBI) compare
b3924 fpr -1.5 -9.1 ferredoxin-NADP reductase (NCBI) compare
b3164 pnp -1.5 -3.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0049 apaH -1.5 -1.0 diadenosinetetraphosphatase (NCBI) compare
b3021 ygiT -1.4 -3.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2020 hisD -1.4 -7.8 histidinol dehydrogenase (NCBI) compare
b4214 cysQ -1.4 -9.4 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b0439 lon -1.4 -3.4 DNA-binding ATP-dependent protease La (NCBI) compare
b1273 yciN -1.4 -2.4 hypothetical protein (NCBI) compare
b3493 pitA -1.4 -6.8 phosphate transporter, low-affinity (NCBI) compare
b2019 hisG -1.4 -7.1 ATP phosphoribosyltransferase (NCBI) compare
b0001 thrL -1.4 -2.1 thr operon leader peptide (NCBI) compare
b3368 cysG -1.4 -10.8 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI) compare
b3772 ilvA -1.3 -11.2 threonine dehydratase (NCBI) compare
b0033 carB -1.3 -3.1 carbamoyl-phosphate synthase large subunit (NCBI) compare
b0071 leuD -1.3 -7.3 isopropylmalate isomerase small subunit (NCBI) compare
b0588 fepC -1.3 -8.3 iron-enterobactin transporter subunit (NCBI) compare
b0741 pal -1.3 -1.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0585 fes -1.3 -7.2 enterobactin/ferric enterobactin esterase (NCBI) compare
b0200 gmhB -1.3 -0.9 hypothetical protein (NCBI) compare
b3838 tatB -1.3 -6.2 sec-independent translocase (NCBI) compare
b3770 ilvE -1.3 -10.1 branched-chain amino acid aminotransferase (NCBI) compare
b3957 argE -1.3 -10.0 acetylornithine deacetylase (NCBI) compare
b3621 rfaC -1.3 -1.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0589 fepG -1.3 -8.9 iron-enterobactin transporter subunit (NCBI) compare
b4529 ydbJ -1.3 -1.6 hypothetical protein (NCBI) compare
b4171 miaA -1.3 -3.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0592 fepB -1.2 -8.9 iron-enterobactin transporter subunit (NCBI) compare
b2414 cysK -1.2 -8.1 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b0113 pdhR -1.2 -6.2 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b1332 ynaJ -1.2 -1.2 predicted inner membrane protein (NCBI) compare
b1175 minD -1.2 -4.1 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1192 ldcA -1.2 -2.7 L,D-carboxypeptidase A (NCBI) compare
b1438 ydcQ -1.2 -2.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3790 rffC -1.2 -3.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b2500 purN -1.2 -4.3 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2313 cvpA -1.2 -2.1 membrane protein required for colicin V production (NCBI) compare
b0590 fepD -1.2 -8.3 iron-enterobactin transporter subunit (NCBI) compare
b3792 wzxE -1.2 -2.3 O-antigen translocase (NCBI) compare
b3791 rffA -1.2 -3.6 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3008 metC -1.2 -8.5 cystathionine beta-lyase (NCBI) compare
b2611 ypjD -1.2 -6.0 predicted inner membrane protein (RefSeq) compare
b2144 sanA -1.2 -9.3 hypothetical protein (NCBI) compare
b2064 asmA -1.2 -5.7 predicted assembly protein (NCBI) compare
b2175 spr -1.1 -2.8 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0074 leuA -1.1 -7.9 2-isopropylmalate synthase (NCBI) compare
b0952 ymbA -1.1 -6.1 conserved protein (RefSeq) compare
b0767 ybhE -1.1 -4.3 6-phosphogluconolactonase (NCBI) compare
b3849 trkH -1.1 -7.6 potassium transporter (NCBI) compare
b0926 ycbK -1.1 -3.8 hypothetical protein (NCBI) compare
b1855 lpxM -1.1 -4.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4593 ymgI -1.1 -1.2 hypothetical protein (RefSeq) compare
b0400 phoR -1.1 -8.4 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b3787 rffD -1.1 -6.2 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1176 minC -1.1 -2.9 septum formation inhibitor (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b0242 proB -1.1 -4.4 gamma-glutamyl kinase (NCBI) compare
b3794 rffM -1.1 -5.1 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3781 trxA -1.1 -4.2 thioredoxin 1 (VIMSS) compare
b0434 yajG -1.0 -5.9 putative polymerase/proteinase (VIMSS) compare
b2818 argA -1.0 -6.7 N-acetylglutamate synthase (NCBI) compare
b3150 yraP -1.0 -5.6 hypothetical protein (NCBI) compare
b0951 pqiB -1.0 -6.5 paraquat-inducible protein B (NCBI) compare
b1264 trpE -1.0 -2.8 anthranilate synthase component I (NCBI) compare
b1865 nudB -1.0 -1.2 dATP pyrophosphohydrolase (NCBI) compare
b4179 vacB -1.0 -3.7 putative enzyme (VIMSS) compare
b1677 lpp -1.0 -2.1 murein lipoprotein (NCBI) compare
b3630 rfaP -1.0 -4.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2340 sixA -1.0 -3.9 phosphohistidine phosphatase (NCBI) compare
b3958 argC -1.0 -7.7 N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) compare
b4481 rffT -0.9 -2.8 4-alpha-L-fucosyltransferase (NCBI) compare
b4655 ythA -0.9 -2.0 expressed protein (RefSeq) compare
b3527 yhjJ -0.9 -8.4 predicted zinc-dependent peptidase (NCBI) compare
b1856 yebA -0.9 -6.6 orf, hypothetical protein (VIMSS) compare
b0912 ihfB -0.9 -0.6 integration host factor subunit beta (NCBI) compare
b0683 fur -0.9 -1.2 ferric uptake regulator (NCBI) compare
b3609 secB -0.9 -2.1 export protein SecB (NCBI) compare
b2479 gcvR -0.9 -4.9 transcriptional regulation of gcv operon (VIMSS) compare
b4676 yoaK -0.9 -0.7 expressed protein (RefSeq) compare
b3959 argB -0.9 -6.6 acetylglutamate kinase (RefSeq) compare
b3786 rffE -0.9 -6.2 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2518 ndk -0.9 -2.0 nucleoside diphosphate kinase (NCBI) compare
b0950 pqiA -0.9 -4.2 paraquat-inducible membrane protein A (NCBI) compare
b3006 exbB -0.9 -5.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2026 hisI -0.9 -5.6 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI) compare
b3993 thiE -0.9 -6.3 thiamine-phosphate pyrophosphorylase (NCBI) compare
b0226 dinJ -0.9 -3.8 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3375 yhfR -0.9 -6.5 putative transcriptional regulator (VIMSS) compare
b3082 ygjM -0.9 -0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b4596 yciZ -0.8 -1.6 hypothetical protein (RefSeq) compare
b3247 cafA -0.8 -6.4 bundles of cytoplasmic filaments (VIMSS) compare
b4702 mgtL -0.8 -1.3 regulatory leader peptide for mgtA (NCBI) compare
b3204 ptsN -0.8 -3.0 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1418 cybB -0.8 -1.2 cytochrome b(561) (VIMSS) compare
b3912 cpxR -0.8 -4.8 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b1084 rne -0.8 -3.1 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0688 pgm -0.8 -2.1 phosphoglucomutase (NCBI) compare
b4169 amiB -0.8 -6.0 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b1551 b1551 -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b3005 exbD -0.8 -2.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4451 ryhB -0.8 -1.8 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0075 leuL -0.8 -4.5 leu operon leader peptide (NCBI) compare
b2498 upp -0.8 -2.5 uracil phosphoribosyltransferase (VIMSS) compare
b3398 yrfF -0.8 -2.3 predicted inner membrane protein (NCBI) compare
b4418 sraB -0.7 -2.2 unknown RNA (NCBI) compare
b1105 ycfM -0.7 -2.9 predicted outer membrane lipoprotein (NCBI) compare
b2951 yggS -0.7 -3.2 predicted enzyme (NCBI) compare
b3911 cpxA -0.7 -4.4 two-component sensor protein (NCBI) compare
b2528 iscA -0.7 -1.3 iron-sulfur cluster assembly protein (NCBI) compare
b3250 mreC -0.7 -1.7 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b3990 thiH -0.7 -4.8 thiamine biosynthesis protein ThiH (NCBI) compare
b1252 tonB -0.7 -2.5 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2103 thiD -0.7 -4.4 phosphomethylpyrimidine kinase (NCBI) compare
b0077 ilvI -0.7 -5.5 acetolactate synthase III large subunit (NCBI) compare
b3465 yhhF -0.7 -3.9 predicted methyltransferase (NCBI) compare
b4375 prfC -0.7 -3.5 peptide chain release factor 3 (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For no stress control Dimethyl Sulfoxide in Escherichia coli BW25113

For no stress control Dimethyl Sulfoxide across organisms