Experiment set53IT016 for Escherichia coli BW25113

Compare to:

M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b2741 rpoS +2.8 25.3 RNA polymerase sigma factor (NCBI) compare
b3929 rraA +2.4 19.7 ribonuclease activity regulator protein RraA (NCBI) compare
b1085 b1085 +2.3 3.1 orf, hypothetical protein (VIMSS) compare
b3210 arcB +2.0 12.9 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b3844 fre +1.9 13.4 NAD(P)H-flavin reductase (NCBI) compare
b1954 dsrA +1.5 7.9 regulatory antisense RNA (NCBI) compare
b2576 srmB +1.4 6.2 ATP-dependent RNA helicase (NCBI) compare
b1830 prc +1.4 7.2 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3163 nlpI +1.4 6.0 hypothetical protein (NCBI) compare
b0990 cspG +1.3 2.1 DNA-binding transcriptional regulator (NCBI) compare
b2168 fruK +1.2 1.4 1-phosphofructokinase (NCBI) compare
b4313 fimE +1.2 9.7 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2934 cmtB +1.2 1.7 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3443 yrhA +1.2 1.3 orf, hypothetical protein (VIMSS) compare
b3198 kdsC +1.2 3.6 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b0122 yacC +1.1 5.9 orf, hypothetical protein (VIMSS) compare
b2684 mprA +1.1 6.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4000 hupA +1.0 6.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3146 yraL +1.0 7.7 predicted methyltransferase (NCBI) compare
b0232 yafN +1.0 0.9 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2688 gshA +1.0 6.0 glutamate--cysteine ligase (NCBI) compare
b2846 yqeH +1.0 1.1 orf, hypothetical protein (VIMSS) compare
b0882 clpA +1.0 8.8 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b4603 rseX +1.0 1.2 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2571 rseB +1.0 6.1 periplasmic negative regulator of sigmaE (NCBI) compare
b0240 crl +1.0 8.1 DNA-binding transcriptional regulator (NCBI) compare
b2531 iscR +1.0 2.7 DNA-binding transcriptional repressor (NCBI) compare
b0925 ycbB +0.9 7.8 predicted carboxypeptidase (NCBI) compare
b1823 cspC +0.9 2.8 stress protein, member of the CspA-family (NCBI) compare
b0955 ycbZ +0.9 7.2 predicted peptidase (NCBI) compare
b1936 intG +0.9 1.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3628 rfaB +0.9 7.3 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2829 ptsP +0.9 8.3 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3162 deaD +0.9 5.1 inducible ATP-independent RNA helicase (VIMSS) compare
b1382 ynbE +0.9 2.6 predicted lipoprotein (NCBI) compare
b0382 yaiB +0.9 6.9 hypothetical protein (NCBI) compare
b1150 ymfR +0.9 1.5 e14 prophage; predicted protein (NCBI) compare
b2217 rcsB +0.8 5.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2512 yfgL +0.8 5.1 protein assembly complex, lipoprotein component (NCBI) compare
b3206 npr +0.8 4.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1182 hlyE +0.8 0.8 hemolysin E (VIMSS) compare
b1269 rluB +0.8 3.3 23S rRNA pseudouridylate synthase (NCBI) compare
b4097 phnK +0.8 2.9 carbon-phosphorus lyase complex subunit (NCBI) compare
b1642 slyA +0.8 3.2 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0178 hlpA +0.8 4.8 periplasmic chaperone (NCBI) compare
b4704 arrS +0.8 0.4 Antisense sRNA ArrS, function unknown (NCBI) compare
b4071 nrfB +0.8 1.8 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1179 ycgL +0.8 2.3 conserved protein (NCBI) compare
b3764 yifE +0.8 4.7 hypothetical protein (NCBI) compare
b4066 yjcF +0.8 0.6 hypothetical protein (NCBI) compare
b3871 bipA +0.7 6.3 GTP-binding protein (NCBI) compare
b1233 ychJ +0.7 3.4 hypothetical protein (NCBI) compare
b0759 galE +0.7 4.6 UDP-galactose-4-epimerase (VIMSS) compare
b3672 ivbL +0.7 1.7 ilvB operon leader peptide (NCBI) compare
b0754 aroG +0.7 4.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b1743 spy +0.7 1.6 envelope stress induced periplasmic protein (NCBI) compare
b4585 sroB +0.7 1.5 Novel sRNA, function unknown (RefSeq) compare
b3753 rbsR +0.7 5.7 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0082 mraW +0.7 4.0 S-adenosyl-methyltransferase (NCBI) compare
b3816 corA +0.7 5.3 magnesium/nickel/cobalt transporter (NCBI) compare
b1381 ydbH +0.7 5.5 hypothetical protein (NCBI) compare
b3199 yrbK +0.7 1.5 hypothetical protein (NCBI) compare
b4480 hdfR +0.7 4.7 transcriptional regulator HdfR (NCBI) compare
b0535 fimZ +0.7 3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3523 yhjE +0.7 5.3 predicted transporter (NCBI) compare
b4692 oweE +0.7 1.4 no description compare
b2617 smpA +0.7 3.3 small membrane protein A (VIMSS) compare
b4133 cadC +0.7 6.0 DNA-binding transcriptional activator (NCBI) compare
b1480 sra +0.7 1.1 30S ribosomal subunit protein S22 (NCBI) compare
b2749 ygbE +0.6 4.2 conserved inner membrane protein (NCBI) compare
b3933 ftsN +0.6 4.7 essential cell division protein (NCBI) compare
b3349 slyD +0.6 4.4 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b4552 yrhC +0.6 1.0 no description compare
b1667 ydhR +0.6 1.7 hypothetical protein (NCBI) compare
b2007 yeeX +0.6 2.6 conserved protein (RefSeq) compare
b1678 ynhG +0.6 4.1 hypothetical protein (NCBI) compare
b4410 ecnA +0.6 1.5 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1346 ydaQ +0.6 1.0 orf, hypothetical protein (VIMSS) compare
b1761 gdhA +0.6 3.5 glutamate dehydrogenase (NCBI) compare
b2786 barA +0.6 5.9 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b1253 yciA +0.6 1.7 predicted hydrolase (NCBI) compare
b0131 panD +0.6 2.5 aspartate 1-decarboxylase precursor (NCBI) compare
b3261 fis +0.6 1.5 DNA-binding protein Fis (NCBI) compare
b3387 dam +0.6 3.8 DNA adenine methylase (NCBI) compare
b4293 fecI +0.6 1.8 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b0503 ybbB +0.6 1.9 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1333 uspE +0.6 3.8 stress-induced protein (NCBI) compare
b1914 uvrY +0.6 3.6 response regulator (NCBI) compare
b1628 rsxB +0.6 1.9 electron transport complex protein RnfB (NCBI) compare
b4679 yohP +0.6 2.6 expressed protein (RefSeq) compare
b1824 yobF +0.6 1.8 hypothetical protein (NCBI) compare
b0998 torD +0.5 1.2 chaperone protein TorD (NCBI) compare
b0440 hupB +0.5 3.4 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b2136 yohD +0.5 3.3 orf, hypothetical protein (VIMSS) compare
b0234 yafP +0.5 1.5 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1725 yniA +0.5 1.9 predicted phosphotransferase/kinase (NCBI) compare
b2944 sprT +0.5 1.9 hypothetical protein (NCBI) compare
b4443 gcvB +0.5 0.3 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b0388 aroL +0.5 3.8 shikimate kinase II (NCBI) compare
b1831 proQ +0.5 2.9 putative solute/DNA competence effector (NCBI) compare
b2363 yfdT +0.5 2.0 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1627 rsxA +0.5 2.5 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1591 b1591 +0.5 1.8 putative oxidoreductase component (VIMSS) compare
b0851 nfsA +0.5 1.9 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1187 fadR +0.5 3.2 fatty acid metabolism regulator (NCBI) compare
b1422 ydcI +0.5 3.0 putative transcriptional regulator LYSR-type (VIMSS) compare
b2314 dedD +0.5 2.0 putative lipoprotein (VIMSS) compare
b4450 arcZ +0.5 2.9 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3755 yieP +0.5 3.2 predicted transcriptional regulator (NCBI) compare
b0991 ymcE +0.5 2.4 cold shock gene (NCBI) compare
b0661 miaB +0.5 4.4 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3234 degQ +0.5 4.0 serine endoprotease, periplasmic (NCBI) compare
b0819 ybiS +0.5 3.4 hypothetical protein (NCBI) compare
b3043 ygiL +0.5 2.8 predicted fimbrial-like adhesin protein (NCBI) compare
b0864 artP +0.5 2.5 arginine transporter subunit (NCBI) compare
b2811 ygdK +0.5 2.1 predicted Fe-S metabolism protein (NCBI) compare
b3495 uspA +0.5 4.1 universal stress global response regulator (NCBI) compare
b0483 ybaQ +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b2042 galF +0.5 3.6 predicted subunit with GalU (NCBI) compare
b1891 flhC +0.5 2.4 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1729 ydjN +0.5 2.3 predicted transporter (NCBI) compare
b3459 yhhK +0.5 2.7 hypothetical protein (NCBI) compare
b1492 gadC +0.5 3.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b0436 tig +0.5 2.4 trigger factor (NCBI) compare
b3512 GadE +0.5 2.3 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1448 yncA +0.5 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3858 yihD +0.5 1.5 hypothetical protein (NCBI) compare
b1217 chaB +0.5 1.0 cation transport regulator (NCBI) compare
b1218 chaC +0.5 2.9 regulatory protein for cation transport (RefSeq) compare
b0432 cyoA +0.4 3.5 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b0505 allA +0.4 1.3 ureidoglycolate hydrolase (NCBI) compare
b1992 cobS +0.4 1.2 cobalamin synthase (NCBI) compare
b2643 yfjX +0.4 1.3 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b4694 yagP +0.4 1.6 no description compare
b2330 yfcB +0.4 1.1 putative adenine-specific methylase (VIMSS) compare
b3627 rfaI +0.4 3.1 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2768 ygcP +0.4 2.0 predicted anti-terminator regulatory protein (NCBI) compare
b2435 amiA +0.4 2.1 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1186 nhaB +0.4 3.2 NhaB sodium/proton transporter (Katherine Huang) compare
b3014 yqhH +0.4 2.7 predicted outer membrane lipoprotein (NCBI) compare
b3961 oxyR +0.4 3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2659 b2659 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3213 gltD +0.4 3.6 glutamate synthase, 4Fe-4S protein, small subunit (NCBI) compare
b2506 yfgI +0.4 1.2 hypothetical protein (NCBI) compare
b1679 sufE +0.4 2.5 cysteine desufuration protein SufE (NCBI) compare
b2448 yffQ +0.4 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b4590 ybfK +0.4 0.6 hypothetical protein (RefSeq) compare
b1942 fliJ +0.4 0.8 flagellar biosynthesis chaperone (NCBI) compare
b1243 oppA +0.4 3.3 oligopeptide transporter subunit (NCBI) compare
b4458 oxyS +0.4 1.1 global regulatory RNA (NCBI) compare
b1164 ycgZ +0.4 1.1 hypothetical protein (NCBI) compare
b4227 ytfQ +0.4 2.1 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b2698 recX +0.4 1.5 RecA regulator RecX (NCBI) compare
b1556 b1556 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b2229 b2229 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b1630 rsxD +0.4 2.0 electron transport complex protein RnfD (NCBI) compare
b2436 hemF +0.4 2.3 coproporphyrinogen III oxidase (NCBI) compare
b1246 oppD +0.4 2.8 oligopeptide transporter ATP-binding component (NCBI) compare
b3341 rpsG +0.4 0.9 30S ribosomal protein S7 (NCBI) compare
b2644 yfjY +0.4 1.2 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b1613 manA +0.4 0.8 mannose-6-phosphate isomerase (VIMSS) compare
b4426 isrA +0.4 1.5 unknown RNA (NCBI) compare
b4255 yjgD +0.4 1.7 hypothetical protein (NCBI) compare
b2286 nuoC +0.4 2.2 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1822 rrmA +0.4 1.2 23S rRNA m1G745 methyltransferase (NCBI) compare
b1648 b1648 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1625 ydgT +0.4 2.2 predicted regulator (NCBI) compare
b2093 gatB +0.4 1.6 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b3009 yghB +0.4 2.2 conserved inner membrane protein (NCBI) compare
b3466 yhhL +0.4 2.4 conserved inner membrane protein (NCBI) compare
b1244 oppB +0.4 2.8 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1446 ydcY +0.4 1.7 hypothetical protein (NCBI) compare
b1818 manY +0.4 1.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1347 ydaC +0.4 0.2 Rac prophage; predicted protein (NCBI) compare
b3194 yrbE +0.4 2.8 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2006 yeeW +0.4 1.9 CP4-44 prophage; predicted protein (RefSeq) compare
b1583 ynfB +0.4 1.9 hypothetical protein (NCBI) compare
b0868 b0868 +0.4 3.1 putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS) compare
b4701 sokX +0.4 0.7 sok-related sRNA, function unknown (RefSeq) compare
b4594 ymgJ +0.4 1.1 hypothetical protein (RefSeq) compare
b2004 yeeU +0.4 1.3 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b4595 yciY +0.4 1.2 hypothetical protein (RefSeq) compare
b3643 rph +0.4 2.0 ribonuclease PH (NCBI) compare
b4402 yjjY +0.4 0.7 hypothetical protein (NCBI) compare
b0425 panE +0.4 3.2 2-dehydropantoate 2-reductase (NCBI) compare
b3718 yieK +0.4 2.3 hypothetical protein (NCBI) compare
b1557 cspB +0.4 1.2 Qin prophage; cold shock protein (NCBI) compare
b1949 fliQ +0.4 1.7 flagellar biosynthesis protein Q (NCBI) compare
b3354 yheU +0.4 2.0 hypothetical protein (NCBI) compare
b1689 b1689 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b0831 yliC +0.4 2.4 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1330 ynaI +0.4 2.0 conserved inner membrane protein (NCBI) compare
b4215 ytfI +0.3 0.8 hypothetical protein (NCBI) compare
b1132 hflD +0.3 1.4 hypothetical protein (NCBI) compare
b1869 yecN +0.3 2.6 orf, hypothetical protein (VIMSS) compare
b1045 ymdB +0.3 1.9 hypothetical protein (NCBI) compare
b2667 ygaV +0.3 2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b0862 artQ +0.3 1.7 arginine transporter subunit (NCBI) compare
b3102 yqjG +0.3 1.8 predicted S-transferase (NCBI) compare
b3516 gadX +0.3 2.9 DNA-binding transcriptional dual regulator (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For no stress control Dimethyl Sulfoxide in Escherichia coli BW25113

For no stress control Dimethyl Sulfoxide across organisms