Experiment set53IT015 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.06 mM 24 hr

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +3.1 22.7 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2741 rpoS +2.4 20.7 RNA polymerase sigma factor (NCBI) compare
b4402 yjjY +2.2 5.9 hypothetical protein (NCBI) compare
b1954 dsrA +2.0 8.3 regulatory antisense RNA (NCBI) compare
b2217 rcsB +1.9 15.7 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b0014 dnaK +1.7 7.1 molecular chaperone DnaK (NCBI) compare
b1823 cspC +1.6 4.2 stress protein, member of the CspA-family (NCBI) compare
b3229 sspA +1.5 8.3 stringent starvation protein A (NCBI) compare
b1642 slyA +1.5 6.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2659 b2659 +1.1 2.6 orf, hypothetical protein (VIMSS) compare
b4133 cadC +1.1 10.4 DNA-binding transcriptional activator (NCBI) compare
b4593 ymgI +1.1 1.3 hypothetical protein (RefSeq) compare
b2531 iscR +1.0 3.4 DNA-binding transcriptional repressor (NCBI) compare
b3443 yrhA +0.9 1.8 orf, hypothetical protein (VIMSS) compare
b3753 rbsR +0.9 8.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b3672 ivbL +0.9 2.9 ilvB operon leader peptide (NCBI) compare
b1765 ydjA +0.9 1.3 predicted oxidoreductase (NCBI) compare
b3390 aroK +0.9 2.1 shikimate kinase I (NCBI) compare
b1730 ydjO +0.9 1.6 predicted protein (RefSeq) compare
b0240 crl +0.9 6.1 DNA-binding transcriptional regulator (NCBI) compare
b3516 gadX +0.8 7.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4546 ypeB +0.8 2.0 hypothetical protein (NCBI) compare
b3512 GadE +0.8 3.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1824 yobF +0.8 3.5 hypothetical protein (NCBI) compare
b4679 yohP +0.8 3.1 expressed protein (RefSeq) compare
b3002 yqhA +0.8 2.2 hypothetical protein (NCBI) compare
b4215 ytfI +0.8 1.9 hypothetical protein (NCBI) compare
b3146 yraL +0.8 6.1 predicted methyltransferase (NCBI) compare
b4704 arrS +0.8 0.4 Antisense sRNA ArrS, function unknown (NCBI) compare
b4071 nrfB +0.8 1.8 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1936 intG +0.8 1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1231 tyrT +0.8 1.5 tRNA-Tyr (NCBI) compare
b1247 oppF +0.7 5.7 oligopeptide transporter subunit (NCBI) compare
b3397 nudE +0.7 6.0 ADP-ribose diphosphatase (NCBI) compare
b4246 pyrL +0.7 1.4 pyrBI operon leader peptide (NCBI) compare
b1658 purR +0.7 5.7 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b4585 sroB +0.7 1.6 Novel sRNA, function unknown (RefSeq) compare
b2934 cmtB +0.7 0.9 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1357 ydaS +0.7 2.7 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b3163 nlpI +0.7 2.4 hypothetical protein (NCBI) compare
b3495 uspA +0.7 6.1 universal stress global response regulator (NCBI) compare
b1233 ychJ +0.7 2.5 hypothetical protein (NCBI) compare
b4545 ypdJ +0.6 1.4 no description compare
b2637 yfjT +0.6 3.5 CP4-57 prophage; predicted protein (NCBI) compare
b0082 mraW +0.6 3.6 S-adenosyl-methyltransferase (NCBI) compare
b1244 oppB +0.6 5.0 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b2189 proL +0.6 2.1 tRNA-Pro (NCBI) compare
b1492 gadC +0.6 4.3 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1160 elbA +0.6 0.8 hypothetical protein (NCBI) compare
b2829 ptsP +0.6 5.8 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2502 ppx +0.6 4.9 exopolyphosphatase (NCBI) compare
b3755 yieP +0.6 3.4 predicted transcriptional regulator (NCBI) compare
b3206 npr +0.6 3.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4603 rseX +0.6 0.7 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4592 yccB +0.6 1.4 hypothetical protein (RefSeq) compare
b2181 yejG +0.6 2.8 hypothetical protein (NCBI) compare
b2510 b2510 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b1245 oppC +0.5 3.6 oligopeptide transporter subunit (NCBI) compare
b4520 ymgF +0.5 1.6 hypothetical protein (NCBI) compare
b1953 b1953 +0.5 3.1 orf, hypothetical protein (VIMSS) compare
b2229 b2229 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.5 2.8 shikimate kinase II (NCBI) compare
b1733 chbG +0.5 2.3 hypothetical protein (NCBI) compare
b4552 yrhC +0.5 0.8 no description compare
b2668 ygaP +0.5 3.9 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1257 yciE +0.5 0.3 hypothetical protein (NCBI) compare
b1848 yebG +0.5 1.3 conserved protein regulated by LexA (NCBI) compare
b0661 miaB +0.5 4.3 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1583 ynfB +0.5 2.5 hypothetical protein (NCBI) compare
b0782 moaB +0.5 2.0 molybdopterin biosynthesis protein B (NCBI) compare
b1246 oppD +0.5 3.6 oligopeptide transporter ATP-binding component (NCBI) compare
b3889 yiiE +0.5 1.7 predicted transcriptional regulator (RefSeq) compare
b1591 b1591 +0.5 1.5 putative oxidoreductase component (VIMSS) compare
b0955 ycbZ +0.5 3.7 predicted peptidase (NCBI) compare
b0696 kdpC +0.5 2.0 potassium-transporting ATPase subunit C (NCBI) compare
b1743 spy +0.5 1.3 envelope stress induced periplasmic protein (NCBI) compare
b1607 ydgC +0.5 1.2 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4532 yncN +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1920 fliY +0.5 2.0 cystine transporter subunit (NCBI) compare
b0866 ybjQ +0.5 1.1 hypothetical protein (NCBI) compare
b1448 yncA +0.5 1.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2960 yggH +0.5 3.5 tRNA(m7G46)-methyltransferase (NCBI) compare
b2007 yeeX +0.5 0.7 conserved protein (RefSeq) compare
b0303 ykgI +0.5 0.5 orf, hypothetical protein (VIMSS) compare
b4650 yibS +0.5 1.7 no description compare
b2006 yeeW +0.4 1.9 CP4-44 prophage; predicted protein (RefSeq) compare
b3429 glgA +0.4 3.7 glycogen synthase (NCBI) compare
b0982 yccY +0.4 2.8 putative phosphatase (VIMSS) compare
b1499 ydeO +0.4 0.4 predicted DNA-binding transcriptional acfivator (NCBI) compare
b0660 ybeZ +0.4 2.5 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b0774 bioA +0.4 2.6 adenosylmethionine--8-amino-7-oxononanoate transaminase (NCBI) compare
b1001 yccE +0.4 0.3 hypothetical protein (NCBI) compare
b4017 arpA +0.4 2.2 regulator of acetyl CoA synthetase (NCBI) compare
b1062 pyrC +0.4 2.1 dihydroorotase (NCBI) compare
b2504 yfgG +0.4 1.1 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1038 csgF +0.4 1.9 predicted transport protein (NCBI) compare
b1243 oppA +0.4 3.3 oligopeptide transporter subunit (NCBI) compare
b1550 b1550 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1725 yniA +0.4 1.8 predicted phosphotransferase/kinase (NCBI) compare
b4050 yjbO +0.4 2.7 orf, hypothetical protein (VIMSS) compare
b4045 yjbJ +0.4 1.3 predicted stress response protein (NCBI) compare
b3779 gpp +0.4 3.9 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b1919 yedO +0.4 1.5 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0617 citD +0.4 1.5 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b0792 ybhR +0.4 2.6 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2448 yffQ +0.4 1.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b3293 yhdN +0.4 1.7 hypothetical protein (NCBI) compare
b0965 b0965 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b2742 nlpD +0.4 2.1 predicted outer membrane lipoprotein (NCBI) compare
b1627 rsxA +0.4 1.9 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2004 yeeU +0.4 1.3 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b2236 yfaE +0.4 2.2 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4279 yjhB +0.4 0.2 putative transport protein (VIMSS) compare
b1040 csgD +0.4 1.0 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b0436 tig +0.4 2.7 trigger factor (NCBI) compare
b2814 metZ +0.4 1.1 tRNA-Met (NCBI) compare
b1671 ydhX +0.4 1.8 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2666 yqaE +0.4 2.3 predicted membrane protein (NCBI) compare
b1461 ydcE +0.4 1.7 4-oxalocrotonate tautomerase (NCBI) compare
b3106 yhaK +0.4 1.7 predicted pirin-related protein (NCBI) compare
b3816 corA +0.4 3.2 magnesium/nickel/cobalt transporter (NCBI) compare
b2635 ypjK +0.4 1.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2936 yggG +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1648 b1648 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4064 yjcD +0.4 3.3 predicted permease (NCBI) compare
b1344 ydaO +0.4 1.1 predicted C32 tRNA thiolase (NCBI) compare
b4529 ydbJ +0.4 0.8 hypothetical protein (NCBI) compare
b4692 oweE +0.4 1.7 no description compare
b4450 arcZ +0.4 1.4 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b0623 cspE +0.4 1.3 cold shock protein E (NCBI) compare
b0104 guaC +0.4 3.0 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b1085 b1085 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b2433 b2433 +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b0503 ybbB +0.4 1.2 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1242 ychE +0.4 2.1 predicted inner membrane protein (NCBI) compare
b2424 cysU +0.4 1.5 sulfate/thiosulfate transporter subunit (NCBI) compare
b2506 yfgI +0.4 1.1 hypothetical protein (NCBI) compare
b4214 cysQ +0.4 2.9 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b3228 sspB +0.4 2.3 ClpXP protease specificity-enhancing factor (NCBI) compare
b3292 zntR +0.4 2.0 zinc-responsive transcriptional regulator (NCBI) compare
b1576 ydfD +0.4 0.2 Qin prophage; predicted protein (NCBI) compare
b4095 phnM +0.4 2.4 carbon-phosphorus lyase complex subunit (NCBI) compare
b1669 ydhT +0.4 1.5 hypothetical protein (NCBI) compare
b1630 rsxD +0.4 1.5 electron transport complex protein RnfD (NCBI) compare
b1263 trpD +0.4 1.0 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b4453 ldrD +0.4 1.5 toxic polypeptide, small (NCBI) compare
b1970 yedX +0.4 1.9 hypothetical protein (NCBI) compare
b1336 ydaH +0.4 2.4 putative pump protein (transport) (VIMSS) compare
b4577 sgrS +0.4 1.4 small antisense RNA (NCBI) compare
b3432 glgB +0.4 3.2 glycogen branching enzyme (NCBI) compare
b4406 yaeP +0.4 1.6 hypothetical protein (NCBI) compare
b4196 ulaD +0.4 1.8 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI) compare
b4094 phnN +0.4 1.6 ribose 1,5-bisphosphokinase (NCBI) compare
b0232 yafN +0.4 0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2644 yfjY +0.3 1.2 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b2136 yohD +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b3933 ftsN +0.3 2.5 essential cell division protein (NCBI) compare
b2290 yfbQ +0.3 2.7 aspartate aminotransferase (NCBI) compare
b3507 dctR +0.3 3.1 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b1842 holE +0.3 1.1 DNA polymerase III, theta subunit (NCBI) compare
b1306 pspC +0.3 1.6 DNA-binding transcriptional activator (NCBI) compare
b1251 yciI +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b4049 dusA +0.3 2.8 tRNA-dihydrouridine synthase A (RefSeq) compare
b4701 sokX +0.3 0.4 sok-related sRNA, function unknown (RefSeq) compare
b1841 yobA +0.3 0.8 hypothetical protein (NCBI) compare
b0301 ykgB +0.3 2.5 orf, hypothetical protein (VIMSS) compare
b3455 livG +0.3 2.0 leucine/isoleucine/valine transporter subunit (NCBI) compare
b3888 yiiD +0.3 2.2 predicted acetyltransferase (NCBI) compare
b4595 yciY +0.3 1.0 hypothetical protein (RefSeq) compare
b3676 yidH +0.3 2.0 conserved inner membrane protein (NCBI) compare
b1831 proQ +0.3 1.4 putative solute/DNA competence effector (NCBI) compare
b2665 ygaU +0.3 1.4 hypothetical protein (NCBI) compare
b1179 ycgL +0.3 0.7 conserved protein (NCBI) compare
b1792 yeaO +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0423 thiI +0.3 2.0 thiamine biosynthesis protein ThiI (NCBI) compare
b2012 yeeD +0.3 1.6 hypothetical protein (NCBI) compare
b2479 gcvR +0.3 1.7 transcriptional regulation of gcv operon (VIMSS) compare
b1853 yebK +0.3 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3133 agaV +0.3 1.1 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b1822 rrmA +0.3 1.1 23S rRNA m1G745 methyltransferase (NCBI) compare
b0755 gpmA +0.3 1.8 phosphoglyceromutase (NCBI) compare
b4676 yoaK +0.3 0.3 expressed protein (RefSeq) compare
b2009 sbmC +0.3 1.5 DNA gyrase inhibitor (NCBI) compare
b1582 ynfA +0.3 1.4 hypothetical protein (NCBI) compare
b3043 ygiL +0.3 1.7 predicted fimbrial-like adhesin protein (NCBI) compare
b4594 ymgJ +0.3 1.2 hypothetical protein (RefSeq) compare
b1348 lar +0.3 1.1 Rac prophage; restriction alleviation protein (NCBI) compare
b2071 yegJ +0.3 1.0 hypothetical protein (NCBI) compare
b0953 rmf +0.3 1.1 ribosome modulation factor (NCBI) compare
b1147 ymfL +0.3 1.1 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1685 ydiH +0.3 0.8 predicted protein (RefSeq) compare
b0644 ybeQ +0.3 2.3 orf, hypothetical protein (VIMSS) compare
b2540 hcaC +0.3 1.1 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b3207 yrbL +0.3 2.0 hypothetical protein (NCBI) compare
b1300 puuC +0.3 2.0 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b0798 ybiA +0.3 1.1 hypothetical protein (NCBI) compare
b2199 ccmC +0.3 1.2 heme exporter subunit (NCBI) compare
b1548 nohA +0.3 0.6 Qin prophage; predicted packaging protein (RefSeq) compare
b3503 arsC +0.3 1.8 arsenate reductase (NCBI) compare
b1361 ydaW +0.3 0.2 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Methotrexate across organisms