Experiment set53IT014 for Escherichia coli BW25113
M9 with Methotrexate 1.98 mM 24 hr
Group: stressMedia: M9 + Methotrexate (1.98 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a_ucsf, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ben Guthrie on 8/11/21
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 26 genes in this experiment
For stress Methotrexate in Escherichia coli BW25113
For stress Methotrexate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose and glucuronate interconversions
- Biosynthesis of steroids
- Purine metabolism
- Tryptophan metabolism
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- One carbon pool by folate
- Methane metabolism
- Folate biosynthesis
- Carotenoid biosynthesis - General
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| tetrahydromonapterin biosynthesis | 4 | 3 | 2 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| tetrahydrofolate biosynthesis I | 3 | 3 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 6 | 3 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 9 | 1 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 11 | 1 |
| peptidoglycan recycling I | 14 | 14 | 1 |
| superpathway of chorismate metabolism | 59 | 54 | 1 |