Experiment set53IT006 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.06 mM 48 hr

200 most important genes:

  gene name fitness t score description  
b2512 yfgL -4.5 -9.4 protein assembly complex, lipoprotein component (NCBI) compare
b2808 gcvA -4.4 -3.1 DNA-binding transcriptional dual regulator (NCBI) compare
b1185 dsbB -4.1 -2.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA -3.4 -8.1 periplasmic protein disulfide isomerase I (NCBI) compare
b1855 lpxM -3.3 -7.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4242 mgtA -3.2 -2.2 magnesium transporter (NCBI) compare
b4171 miaA -3.0 -4.0 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2312 purF -2.9 -8.2 amidophosphoribosyltransferase (NCBI) compare
b4702 mgtL -2.8 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b0222 lpcA -2.7 -1.9 phosphoheptose isomerase (NCBI) compare
b2571 rseB -2.7 -11.2 periplasmic negative regulator of sigmaE (NCBI) compare
b0439 lon -2.6 -3.0 DNA-binding ATP-dependent protease La (NCBI) compare
b1882 cheY -2.6 -1.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3844 fre -2.5 -9.7 NAD(P)H-flavin reductase (NCBI) compare
b2017 yefM -2.4 -1.4 orf, hypothetical protein (VIMSS) compare
b0688 pgm -2.4 -3.6 phosphoglucomutase (NCBI) compare
b1291 sapD -2.2 -2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0632 dacA -2.2 -10.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1438 ydcQ -2.2 -4.1 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0683 fur -2.2 -2.1 ferric uptake regulator (NCBI) compare
b3052 rfaE -2.2 -2.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3493 pitA -2.1 -8.1 phosphate transporter, low-affinity (NCBI) compare
b1292 sapC -2.1 -2.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0145 dksA -2.1 -4.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0080 fruR -2.0 -7.7 DNA-binding transcriptional dual regulator (NCBI) compare
b2501 ppk -2.0 -11.2 polyphosphate kinase (NCBI) compare
b3164 pnp -1.9 -4.8 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2318 truA -1.9 -3.4 tRNA pseudouridine synthase A (NCBI) compare
b0438 clpX -1.9 -3.5 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0142 folK -1.9 -1.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3979 thrT -1.9 -0.7 tRNA-Thr (NCBI) compare
b3169 nusA -1.9 -2.1 transcription elongation factor NusA (NCBI) compare
b2330 yfcB -1.8 -3.2 putative adenine-specific methylase (VIMSS) compare
b1235 rssB -1.8 -7.0 response regulator of RpoS (NCBI) compare
b4676 yoaK -1.7 -1.0 expressed protein (RefSeq) compare
b4006 purH -1.7 -10.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4203 rplI -1.7 -7.5 50S ribosomal protein L9 (NCBI) compare
b2329 aroC -1.7 -8.1 chorismate synthase (NCBI) compare
b0386 proC -1.7 -3.4 pyrroline-5-carboxylate reductase (NCBI) compare
b0908 aroA -1.6 -8.4 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3916 pfkA -1.6 -3.9 6-phosphofructokinase (NCBI) compare
b3183 obgE -1.6 -2.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4005 purD -1.6 -9.5 phosphoribosylamine--glycine ligase (NCBI) compare
b2784 relA -1.6 -11.3 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b0522 purK -1.6 -7.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b0726 sucA -1.6 -5.9 alpha-ketoglutarate decarboxylase (NCBI) compare
b4375 prfC -1.6 -6.5 peptide chain release factor 3 (NCBI) compare
b0437 clpP -1.6 -1.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2518 ndk -1.5 -3.4 nucleoside diphosphate kinase (NCBI) compare
b0049 apaH -1.5 -1.0 diadenosinetetraphosphatase (NCBI) compare
b1865 nudB -1.5 -1.7 dATP pyrophosphohydrolase (NCBI) compare
b2912 ygfA -1.5 -2.9 putative ligase (VIMSS) compare
b3870 glnA -1.5 -3.3 glutamine synthetase (NCBI) compare
b0019 nhaA -1.5 -11.0 pH-dependent sodium/proton antiporter (NCBI) compare
b0400 phoR -1.5 -10.4 sensory histidine kinase in two-component regulatory system with PhoB (NCBI) compare
b2288 nuoA -1.5 -4.4 NADH dehydrogenase I chain A (VIMSS) compare
b3867 hemN -1.5 -10.5 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b3341 rpsG -1.5 -1.7 30S ribosomal protein S7 (NCBI) compare
b2277 nuoM -1.4 -6.7 NADH dehydrogenase subunit M (NCBI) compare
b0226 dinJ -1.4 -5.4 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1176 minC -1.4 -3.0 septum formation inhibitor (NCBI) compare
b3621 rfaC -1.4 -1.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b4175 hflC -1.4 -9.2 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1025 ycdT -1.4 -2.6 predicted diguanylate cyclase (NCBI) compare
b2414 cysK -1.4 -7.9 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b0889 lrp -1.4 -3.3 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b0053 surA -1.4 -0.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3630 rfaP -1.4 -5.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1332 ynaJ -1.4 -1.3 predicted inner membrane protein (NCBI) compare
b3790 rffC -1.4 -3.3 TDP-fucosamine acetyltransferase (RefSeq) compare
b3792 wzxE -1.4 -2.0 O-antigen translocase (NCBI) compare
b0200 gmhB -1.4 -0.9 hypothetical protein (NCBI) compare
b1084 rne -1.4 -3.7 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3631 rfaG -1.3 -7.9 glucosyltransferase I (NCBI) compare
b3162 deaD -1.3 -5.4 inducible ATP-independent RNA helicase (VIMSS) compare
b3849 trkH -1.3 -9.4 potassium transporter (NCBI) compare
b4174 hflK -1.3 -8.6 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2281 nuoI -1.3 -3.7 NADH dehydrogenase subunit I (NCBI) compare
b2168 fruK -1.3 -1.3 1-phosphofructokinase (NCBI) compare
b3204 ptsN -1.3 -5.2 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2282 nuoH -1.3 -3.9 NADH dehydrogenase subunit H (NCBI) compare
b3620 rfaF -1.3 -2.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3842 rfaH -1.3 -3.7 transcriptional activator RfaH (NCBI) compare
b2287 nuoB -1.2 -4.2 NADH dehydrogenase subunit B (NCBI) compare
b1290 sapF -1.2 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0741 pal -1.2 -1.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0550 rusA -1.2 -3.1 DLP12 prophage; endonuclease RUS (NCBI) compare
b0767 ybhE -1.2 -4.6 6-phosphogluconolactonase (NCBI) compare
b4414 tff -1.2 -3.2 unknown RNA (NCBI) compare
b3791 rffA -1.2 -3.6 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0630 lipB -1.2 -1.3 protein of lipoate biosynthesis (VIMSS) compare
b1109 ndh -1.2 -4.0 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b4654 yjdQ -1.2 -5.1 no description compare
b2683 ygaH -1.1 -3.8 predicted inner membrane protein (NCBI) compare
b2276 nuoN -1.1 -5.4 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b2572 rseA -1.1 -2.3 anti-sigma factor (NCBI) compare
b3787 rffD -1.1 -6.7 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2285 nuoE -1.1 -1.7 NADH dehydrogenase subunit E (NCBI) compare
b1236 galU -1.1 -2.1 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1293 sapB -1.1 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4202 rpsR -1.1 -0.7 30S ribosomal protein S18 (NCBI) compare
b4025 pgi -1.1 -7.9 glucose-6-phosphate isomerase (NCBI) compare
b4180 rlmB -1.1 -6.3 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b4179 vacB -1.1 -4.1 putative enzyme (VIMSS) compare
b2313 cvpA -1.1 -2.8 membrane protein required for colicin V production (NCBI) compare
b4146 yjeK -1.0 -2.0 predicted lysine aminomutase (NCBI) compare
b2278 nuoL -1.0 -6.6 NADH dehydrogenase subunit L (NCBI) compare
b3977 tyrU -1.0 -1.0 tRNA-Tyr (NCBI) compare
b2280 nuoJ -1.0 -4.6 NADH dehydrogenase subunit J (NCBI) compare
b3786 rffE -1.0 -7.2 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1850 eda -1.0 -1.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2286 nuoC -1.0 -4.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3387 dam -1.0 -4.8 DNA adenine methylase (NCBI) compare
b2283 nuoG -1.0 -5.3 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b1090 plsX -1.0 -5.0 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1192 ldcA -1.0 -2.3 L,D-carboxypeptidase A (NCBI) compare
b0912 ihfB -1.0 -0.6 integration host factor subunit beta (NCBI) compare
b3205 yhbJ -1.0 -3.7 hypothetical protein (NCBI) compare
b2188 yejM -1.0 -7.2 predicted hydrolase, inner membrane (NCBI) compare
b2279 nuoK -1.0 -1.9 NADH dehydrogenase subunit K (NCBI) compare
b0101 yacG -1.0 -3.0 zinc-binding protein (NCBI) compare
b3414 gntY -1.0 -4.9 predicted gluconate transport associated protein (NCBI) compare
b0677 nagA -0.9 -4.9 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b3806 cyaA -0.9 -4.2 adenylate cyclase (NCBI) compare
b4041 plsB -0.9 -2.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2951 yggS -0.9 -3.2 predicted enzyme (NCBI) compare
b3398 yrfF -0.9 -3.0 predicted inner membrane protein (NCBI) compare
b0926 ycbK -0.9 -4.6 hypothetical protein (NCBI) compare
b0675 nagD -0.9 -5.5 UMP phosphatase (NCBI) compare
b2421 cysM -0.9 -3.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b2499 purM -0.9 -3.8 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2175 spr -0.9 -2.2 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2340 sixA -0.9 -3.7 phosphohistidine phosphatase (NCBI) compare
b1351 racC -0.9 -0.6 Rac prophage; predicted protein (NCBI) compare
b3247 cafA -0.8 -6.9 bundles of cytoplasmic filaments (VIMSS) compare
b1778 yeaA -0.8 -1.8 methionine sulfoxide reductase B (NCBI) compare
b0564 appY -0.8 -0.8 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0051 ksgA -0.8 -3.6 dimethyladenosine transferase (NCBI) compare
b2553 glnB -0.8 -4.4 regulatory protein P-II for glutamine synthetase (NCBI) compare
b3838 tatB -0.8 -4.4 sec-independent translocase (NCBI) compare
b2569 lepA -0.8 -2.9 GTP-binding protein LepA (NCBI) compare
b4201 priB -0.8 -2.2 primosomal replication protein N (NCBI) compare
b3778 rep -0.8 -4.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1551 b1551 -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b2504 yfgG -0.8 -1.5 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3742 mioC -0.8 -3.3 flavodoxin (NCBI) compare
b1676 pykF -0.8 -3.2 pyruvate kinase (NCBI) compare
b0724 sdhB -0.8 -3.7 succinate dehydrogenase, FeS subunit (NCBI) compare
b0722 sdhD -0.8 -2.8 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4481 rffT -0.8 -2.3 4-alpha-L-fucosyltransferase (NCBI) compare
b2576 srmB -0.8 -2.0 ATP-dependent RNA helicase (NCBI) compare
b3930 menA -0.8 -6.9 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b3494 yhiO -0.8 -4.8 universal stress protein UspB (NCBI) compare
b1874 cutC -0.8 -0.7 copper homeostasis protein (NCBI) compare
b2707 srlR -0.8 -4.6 DNA-bindng transcriptional repressor (NCBI) compare
b4178 yjeB -0.7 -3.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0928 aspC -0.7 -4.9 aspartate aminotransferase, PLP-dependent (NCBI) compare
b4294 insA-7 -0.7 -1.5 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b1281 pyrF -0.7 -1.5 orotidine 5'-phosphate decarboxylase (NCBI) compare
b2617 smpA -0.7 -3.0 small membrane protein A (VIMSS) compare
b1107 nagZ -0.7 -3.6 beta-hexosaminidase (NCBI) compare
b2498 upp -0.7 -2.1 uracil phosphoribosyltransferase (VIMSS) compare
b1982 amn -0.7 -5.6 AMP nucleosidase (NCBI) compare
b1871 yecP -0.7 -3.2 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b3236 mdh -0.7 -4.2 malate dehydrogenase (NCBI) compare
b4388 serB -0.7 -4.6 3-phosphoserine phosphatase (NCBI) compare
b4480 hdfR -0.7 -4.1 transcriptional regulator HdfR (NCBI) compare
b2904 gcvH -0.7 -1.9 glycine cleavage system protein H (NCBI) compare
b3203 yhbH -0.7 -2.3 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b0729 sucD -0.7 -1.8 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2642 yfjW -0.7 -0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0143 pcnB -0.7 -3.4 poly(A) polymerase I (VIMSS) compare
b2809 b2809 -0.7 -2.4 orf, hypothetical protein (VIMSS) compare
b2082 ogrK -0.7 -1.2 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b3965 trmA -0.7 -5.6 tRNA (uracil-5-)-methyltransferase (NCBI) compare
b4390 nadR -0.7 -5.3 probable nadAB transcriptional regulator (VIMSS) compare
b4570 lomR -0.7 -1.6 no description compare
b0563 tfaX -0.7 -4.0 DLP12 prophage; predicted protein (RefSeq) compare
b2906 visC -0.7 -3.7 hypothetical protein (NCBI) compare
b0209 yafD -0.7 -4.3 hypothetical protein (NCBI) compare
b2493 yfgO -0.7 -4.8 predicted inner membrane protein (NCBI) compare
b3021 ygiT -0.7 -1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3237 argR -0.7 -2.5 arginine repressor (NCBI) compare
b3857 mobA -0.7 -4.4 molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI) compare
b3794 rffM -0.7 -3.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2113 mrp -0.6 -4.1 putative ATPase (VIMSS) compare
b0009 mog -0.6 -4.8 molybdenum cofactor biosynthesis protein (NCBI) compare
b3764 yifE -0.6 -2.8 hypothetical protein (NCBI) compare
b3465 yhhF -0.6 -3.2 predicted methyltransferase (NCBI) compare
b0783 moaC -0.6 -3.6 molybdenum cofactor biosynthesis protein C (NCBI) compare
b3911 cpxA -0.6 -2.9 two-component sensor protein (NCBI) compare
b3706 trmE -0.6 -1.7 tRNA modification GTPase (NCBI) compare
b0888 trxB -0.6 -2.7 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3628 rfaB -0.6 -4.6 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b4000 hupA -0.6 -2.9 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0728 sucC -0.6 -2.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b2905 gcvT -0.6 -4.3 glycine cleavage system aminomethyltransferase T (NCBI) compare
b4224 chpS -0.6 -2.8 suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS) compare
b4139 aspA -0.6 -4.8 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b0723 sdhA -0.6 -4.3 succinate dehydrogenase flavoprotein subunit (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms