Experiment set53IT006 for Escherichia coli BW25113

Compare to:

BHIS with Methotrexate 0.06 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +3.1 22.8 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1954 dsrA +2.2 10.5 regulatory antisense RNA (NCBI) compare
b4402 yjjY +2.0 4.7 hypothetical protein (NCBI) compare
b2217 rcsB +1.7 13.8 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2741 rpoS +1.6 12.6 RNA polymerase sigma factor (NCBI) compare
b1823 cspC +1.4 5.4 stress protein, member of the CspA-family (NCBI) compare
b1150 ymfR +1.4 2.8 e14 prophage; predicted protein (NCBI) compare
b0014 dnaK +1.3 4.8 molecular chaperone DnaK (NCBI) compare
b4552 yrhC +1.2 2.1 no description compare
b1642 slyA +1.1 4.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4133 cadC +1.1 9.9 DNA-binding transcriptional activator (NCBI) compare
b2531 iscR +1.0 2.5 DNA-binding transcriptional repressor (NCBI) compare
b3229 sspA +1.0 4.7 stringent starvation protein A (NCBI) compare
b2136 yohD +0.9 4.1 orf, hypothetical protein (VIMSS) compare
b2659 b2659 +0.9 2.1 orf, hypothetical protein (VIMSS) compare
b1824 yobF +0.9 3.1 hypothetical protein (NCBI) compare
b3753 rbsR +0.9 7.5 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b4699 fnrS +0.8 1.3 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1942 fliJ +0.8 2.4 flagellar biosynthesis chaperone (NCBI) compare
b3516 gadX +0.8 7.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0240 crl +0.8 5.7 DNA-binding transcriptional regulator (NCBI) compare
b2934 cmtB +0.8 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3002 yqhA +0.8 2.0 hypothetical protein (NCBI) compare
b2911 ssrS +0.8 1.1 6S regulatory RNA (NCBI) compare
b3755 yieP +0.8 3.8 predicted transcriptional regulator (NCBI) compare
b3206 npr +0.7 3.3 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0082 mraW +0.7 3.8 S-adenosyl-methyltransferase (NCBI) compare
b2181 yejG +0.7 3.8 hypothetical protein (NCBI) compare
b1765 ydjA +0.7 1.0 predicted oxidoreductase (NCBI) compare
b1658 purR +0.7 5.4 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3397 nudE +0.7 5.0 ADP-ribose diphosphatase (NCBI) compare
b1246 oppD +0.7 5.1 oligopeptide transporter ATP-binding component (NCBI) compare
b3146 yraL +0.7 5.1 predicted methyltransferase (NCBI) compare
b3512 GadE +0.7 2.6 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4066 yjcF +0.7 0.5 hypothetical protein (NCBI) compare
b3863 polA +0.7 0.7 DNA polymerase I (NCBI) compare
b2829 ptsP +0.6 6.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2189 proL +0.6 2.2 tRNA-Pro (NCBI) compare
b1245 oppC +0.6 4.9 oligopeptide transporter subunit (NCBI) compare
b1233 ychJ +0.6 2.0 hypothetical protein (NCBI) compare
b0388 aroL +0.6 3.5 shikimate kinase II (NCBI) compare
b2221 atoD +0.6 3.5 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b2093 gatB +0.6 3.7 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2502 ppx +0.6 4.7 exopolyphosphatase (NCBI) compare
b1607 ydgC +0.6 1.6 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b3082 ygjM +0.6 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1179 ycgL +0.6 2.6 conserved protein (NCBI) compare
b0436 tig +0.6 3.5 trigger factor (NCBI) compare
b4345 mcrC +0.6 1.0 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1244 oppB +0.6 4.6 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b4407 thiS +0.6 2.0 sulfur carrier protein ThiS (NCBI) compare
b2666 yqaE +0.6 2.4 predicted membrane protein (NCBI) compare
b4071 nrfB +0.5 1.8 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1992 cobS +0.5 1.2 cobalamin synthase (NCBI) compare
b4426 isrA +0.5 2.3 unknown RNA (NCBI) compare
b3163 nlpI +0.5 2.6 hypothetical protein (NCBI) compare
b1243 oppA +0.5 3.7 oligopeptide transporter subunit (NCBI) compare
b1860 ruvB +0.5 0.4 Holliday junction DNA helicase B (NCBI) compare
b2229 b2229 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1953 b1953 +0.5 2.8 orf, hypothetical protein (VIMSS) compare
b0122 yacC +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b1269 rluB +0.5 3.0 23S rRNA pseudouridylate synthase (NCBI) compare
b1306 pspC +0.5 2.2 DNA-binding transcriptional activator (NCBI) compare
b0263 afuB +0.5 2.4 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b3500 gor +0.5 1.2 glutathione reductase (NCBI) compare
b3495 uspA +0.5 4.3 universal stress global response regulator (NCBI) compare
b1247 oppF +0.5 3.3 oligopeptide transporter subunit (NCBI) compare
b1429 tehA +0.5 1.3 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b2953 yggU +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1358 ydaT +0.5 1.3 Rac prophage; predicted protein (NCBI) compare
b2668 ygaP +0.5 3.8 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1422 ydcI +0.5 2.2 putative transcriptional regulator LYSR-type (VIMSS) compare
b0705 ybfL +0.5 0.5 putative receptor protein (VIMSS) compare
b2960 yggH +0.5 3.4 tRNA(m7G46)-methyltransferase (NCBI) compare
b4590 ybfK +0.5 0.7 hypothetical protein (RefSeq) compare
b1597 asr +0.5 1.9 acid shock protein (VIMSS) compare
b1743 spy +0.5 1.3 envelope stress induced periplasmic protein (NCBI) compare
b0976 hyaE +0.5 2.4 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b2433 b2433 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1919 yedO +0.5 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0623 cspE +0.5 1.2 cold shock protein E (NCBI) compare
b1591 b1591 +0.5 1.7 putative oxidoreductase component (VIMSS) compare
b3884 yihW +0.5 3.4 putative DEOR-type transcriptional regulator (VIMSS) compare
b1492 gadC +0.4 3.2 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b3779 gpp +0.4 4.1 guanosine pentaphosphatase/exopolyphosphatase (NCBI) compare
b0851 nfsA +0.4 2.3 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b3429 glgA +0.4 3.8 glycogen synthase (NCBI) compare
b1831 proQ +0.4 2.4 putative solute/DNA competence effector (NCBI) compare
b0696 kdpC +0.4 1.5 potassium-transporting ATPase subunit C (NCBI) compare
b1001 yccE +0.4 0.3 hypothetical protein (NCBI) compare
b3390 aroK +0.4 0.9 shikimate kinase I (NCBI) compare
b1595 ynfL +0.4 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3032 cpdA +0.4 3.4 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b0982 yccY +0.4 2.3 putative phosphatase (VIMSS) compare
b4214 cysQ +0.4 3.5 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b2212 alkB +0.4 1.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b1330 ynaI +0.4 2.5 conserved inner membrane protein (NCBI) compare
b4045 yjbJ +0.4 1.5 predicted stress response protein (NCBI) compare
b1524 yneH +0.4 0.8 predicted glutaminase (NCBI) compare
b1536 ydeI +0.4 0.7 hypothetical protein (NCBI) compare
b1305 pspB +0.4 1.2 phage shock protein B (NCBI) compare
b0965 b0965 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b4532 yncN +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b4397 creA +0.4 1.2 hypothetical protein (NCBI) compare
b3959 argB +0.4 2.8 acetylglutamate kinase (RefSeq) compare
b3446 yrhB +0.4 2.1 hypothetical protein (NCBI) compare
b0864 artP +0.4 1.7 arginine transporter subunit (NCBI) compare
b4655 ythA +0.4 0.5 expressed protein (RefSeq) compare
b2759 ygcK +0.4 2.5 hypothetical protein (NCBI) compare
b3858 yihD +0.4 1.3 hypothetical protein (NCBI) compare
b4572 ylbE +0.4 2.0 no description compare
b2742 nlpD +0.4 2.4 predicted outer membrane lipoprotein (NCBI) compare
b4606 ypfM +0.4 2.4 hypothetical protein (RefSeq) compare
b0802 ybiJ +0.4 0.9 hypothetical protein (NCBI) compare
b1482 osmC +0.4 1.4 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3071 yqjI +0.4 1.6 predicted transcriptional regulator (NCBI) compare
b3443 yrhA +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1317 ycjU +0.4 2.3 predicted beta-phosphoglucomutase (NCBI) compare
b0104 guaC +0.4 3.2 guanosine 5'-monophosphate oxidoreductase (NCBI) compare
b4577 sgrS +0.4 1.4 small antisense RNA (NCBI) compare
b0661 miaB +0.4 3.2 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b4049 dusA +0.4 2.8 tRNA-dihydrouridine synthase A (RefSeq) compare
b0660 ybeZ +0.4 2.4 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b1250 kch +0.4 2.7 voltage-gated potassium channel (NCBI) compare
b1160 elbA +0.4 0.6 hypothetical protein (NCBI) compare
b1007 b1007 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0821 ybiU +0.4 2.6 hypothetical protein (NCBI) compare
b2510 b2510 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b2993 hybD +0.4 1.3 predicted maturation element for hydrogenase 2 (NCBI) compare
b3507 dctR +0.4 3.2 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b2479 gcvR +0.4 2.1 transcriptional regulation of gcv operon (VIMSS) compare
b1186 nhaB +0.4 2.6 NhaB sodium/proton transporter (Katherine Huang) compare
b2004 yeeU +0.4 1.2 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1151 ymfO +0.4 1.6 e14 prophage; conserved protein (RefSeq) compare
b1382 ynbE +0.4 1.0 predicted lipoprotein (NCBI) compare
b4403 yjtD +0.4 2.3 predicted rRNA methyltransferase (NCBI) compare
b0300 ykgA +0.4 1.7 putative ARAC-type regulatory protein (VIMSS) compare
b4222 ytfP +0.4 1.5 hypothetical protein (NCBI) compare
b4124 dcuR +0.4 2.9 DNA-binding response regulator in two-component regulatory system with DcuS (NCBI) compare
b2690 yqaB +0.3 1.7 predicted hydrolase (NCBI) compare
b4538 yoeF +0.3 1.0 hypothetical protein (RefSeq) compare
b4062 soxS +0.3 1.2 DNA-binding transcriptional dual regulator (NCBI) compare
b4387 ytjB +0.3 2.1 hypothetical protein (NCBI) compare
b1394 paaG +0.3 1.6 enoyl-CoA hydratase (NCBI) compare
b4585 sroB +0.3 0.7 Novel sRNA, function unknown (RefSeq) compare
b2055 wcaE +0.3 0.2 predicted glycosyl transferase (NCBI) compare
b4536 yobH +0.3 2.0 hypothetical protein (NCBI) compare
b1830 prc +0.3 1.5 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0803 ybiI +0.3 1.6 hypothetical protein (NCBI) compare
b1835 yebU +0.3 2.1 putative nucleolar proteins (VIMSS) compare
b1324 tpx +0.3 1.9 thiol peroxidase (NCBI) compare
b0217 yafT +0.3 2.0 predicted aminopeptidase (NCBI) compare
b3432 glgB +0.3 2.9 glycogen branching enzyme (NCBI) compare
b0991 ymcE +0.3 1.6 cold shock gene (NCBI) compare
b2998 yghW +0.3 1.7 hypothetical protein (NCBI) compare
b1950 fliR +0.3 1.5 flagellar biosynthesis protein R (NCBI) compare
b1841 yobA +0.3 1.1 hypothetical protein (NCBI) compare
b2814 metZ +0.3 0.8 tRNA-Met (NCBI) compare
b3752 rbsK +0.3 2.4 ribokinase (NCBI) compare
b1773 ydjI +0.3 1.1 predicted aldolase (NCBI) compare
b1271 yciK +0.3 1.4 short chain dehydrogenase (NCBI) compare
b0850 ybjC +0.3 1.6 predicted inner membrane protein (NCBI) compare
b1516 lsrB +0.3 1.9 AI2 transporter (NCBI) compare
b3816 corA +0.3 2.6 magnesium/nickel/cobalt transporter (NCBI) compare
b1755 ynjC +0.3 1.8 fused transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b4094 phnN +0.3 1.6 ribose 1,5-bisphosphokinase (NCBI) compare
b1480 sra +0.3 0.7 30S ribosomal subunit protein S22 (NCBI) compare
b0225 yafQ +0.3 1.6 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4595 yciY +0.3 1.2 hypothetical protein (RefSeq) compare
b1602 pntB +0.3 1.9 pyridine nucleotide transhydrogenase (NCBI) compare
b2195 ccmG +0.3 1.5 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b3934 cytR +0.3 2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b1619 hdhA +0.3 2.6 7-alpha-hydroxysteroid dehydrogenase (NCBI) compare
b0636 ybeA +0.3 2.1 hypothetical protein (NCBI) compare
b3471 yhhQ +0.3 1.8 conserved inner membrane protein (NCBI) compare
b1213 ychQ +0.3 1.7 predicted transcriptional regulator (NCBI) compare
b0310 ykgH +0.3 0.7 predicted inner membrane protein (NCBI) compare
b1586 b1586 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1172 ymgG +0.3 1.8 conserved protein, UPF0757 family (RefSeq) compare
b3354 yheU +0.3 2.0 hypothetical protein (NCBI) compare
b1198 dhaM +0.3 1.6 fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component (RefSeq) compare
b1821 b1821 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4559 yjdO +0.3 1.4 hypothetical protein (NCBI) compare
b0955 ycbZ +0.3 2.2 predicted peptidase (NCBI) compare
b1219 ychN +0.3 1.2 hypothetical protein (NCBI) compare
b1448 yncA +0.3 0.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1075 flgD +0.3 1.7 flagellar basal body rod modification protein D (NCBI) compare
b3912 cpxR +0.3 2.1 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b4293 fecI +0.3 1.4 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b0279 yagM +0.3 0.2 CP4-6 prophage; predicted protein (NCBI) compare
b2341 yfcX +0.3 2.5 fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI) compare
b0450 glnK +0.3 0.8 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b4125 dcuS +0.3 2.4 sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration (NCBI) compare
b0829 gsiA +0.3 2.2 fused predicted peptide transport subunits of ABC superfamily: ATP-binding components (RefSeq) compare
b1540 ydfH +0.3 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2698 recX +0.3 0.8 RecA regulator RecX (NCBI) compare
b1667 ydhR +0.3 0.6 hypothetical protein (NCBI) compare
b2458 eutI +0.3 2.2 predicted phosphotransacetylase subunit (NCBI) compare
b1814 sdaA +0.3 1.2 L-serine deaminase I (NCBI) compare
b2643 yfjX +0.3 1.3 CP4-57 prophage; predicted antirestriction protein (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms