Experiment set53IT005 for Escherichia coli BW25113

Compare to:

M9 with Methotrexate 0.1 mM 48 hr

200 most detrimental genes:

  gene name fitness t score description  
b2846 yqeH +1.6 1.9 orf, hypothetical protein (VIMSS) compare
b3210 arcB +1.5 11.0 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b3929 rraA +1.5 11.6 ribonuclease activity regulator protein RraA (NCBI) compare
b2741 rpoS +1.3 10.4 RNA polymerase sigma factor (NCBI) compare
b3933 ftsN +1.2 7.9 essential cell division protein (NCBI) compare
b1085 b1085 +1.1 2.8 orf, hypothetical protein (VIMSS) compare
b1600 mdtJ +1.1 2.7 multidrug efflux system transporter (NCBI) compare
b2684 mprA +1.1 3.5 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0122 yacC +1.0 5.8 orf, hypothetical protein (VIMSS) compare
b2531 iscR +1.0 2.2 DNA-binding transcriptional repressor (NCBI) compare
b3146 yraL +0.9 6.9 predicted methyltransferase (NCBI) compare
b2571 rseB +0.9 6.2 periplasmic negative regulator of sigmaE (NCBI) compare
b1954 dsrA +0.9 3.3 regulatory antisense RNA (NCBI) compare
b4313 fimE +0.9 6.9 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1830 prc +0.8 4.0 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1961 dcm +0.8 1.4 DNA cytosine methylase (NCBI) compare
b3443 yrhA +0.8 1.1 orf, hypothetical protein (VIMSS) compare
b2829 ptsP +0.8 7.5 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2007 yeeX +0.8 1.4 conserved protein (RefSeq) compare
b2314 dedD +0.8 3.8 putative lipoprotein (VIMSS) compare
b2217 rcsB +0.8 5.8 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1607 ydgC +0.8 2.5 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1936 intG +0.8 1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2279 nuoK +0.8 2.0 NADH dehydrogenase subunit K (NCBI) compare
b3206 npr +0.7 4.4 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0955 ycbZ +0.7 5.8 predicted peptidase (NCBI) compare
b2659 b2659 +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b2749 ygbE +0.7 5.4 conserved inner membrane protein (NCBI) compare
b0131 panD +0.7 3.4 aspartate 1-decarboxylase precursor (NCBI) compare
b0178 hlpA +0.7 4.7 periplasmic chaperone (NCBI) compare
b4000 hupA +0.7 3.8 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0925 ycbB +0.7 5.0 predicted carboxypeptidase (NCBI) compare
b3844 fre +0.6 4.2 NAD(P)H-flavin reductase (NCBI) compare
b1160 elbA +0.6 1.1 hypothetical protein (NCBI) compare
b4133 cadC +0.6 5.7 DNA-binding transcriptional activator (NCBI) compare
b0232 yafN +0.6 0.4 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3349 slyD +0.6 4.0 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b1520 b1520 +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b1630 rsxD +0.6 3.8 electron transport complex protein RnfD (NCBI) compare
b1382 ynbE +0.6 1.7 predicted lipoprotein (NCBI) compare
b2071 yegJ +0.6 1.8 hypothetical protein (NCBI) compare
b1381 ydbH +0.6 4.7 hypothetical protein (NCBI) compare
b1247 oppF +0.6 4.1 oligopeptide transporter subunit (NCBI) compare
b4215 ytfI +0.6 1.4 hypothetical protein (NCBI) compare
b0082 mraW +0.6 3.5 S-adenosyl-methyltransferase (NCBI) compare
b3163 nlpI +0.6 2.6 hypothetical protein (NCBI) compare
b2688 gshA +0.6 4.2 glutamate--cysteine ligase (NCBI) compare
b3009 yghB +0.6 3.8 conserved inner membrane protein (NCBI) compare
b4590 ybfK +0.6 0.8 hypothetical protein (RefSeq) compare
b1575 dicB +0.6 1.3 Qin prophage; cell division inhibition protein (NCBI) compare
b1824 yobF +0.6 2.8 hypothetical protein (NCBI) compare
b0685 ybfE +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b1380 ldhA +0.6 1.9 D-lactate dehydrogenase (NCBI) compare
b4585 sroB +0.5 1.2 Novel sRNA, function unknown (RefSeq) compare
b1205 ychH +0.5 1.1 predicted inner membrane protein (NCBI) compare
b1758 b1758 +0.5 1.2 putative cytochrome oxidase (VIMSS) compare
b2617 smpA +0.5 2.5 small membrane protein A (VIMSS) compare
b1246 oppD +0.5 3.8 oligopeptide transporter ATP-binding component (NCBI) compare
b2512 yfgL +0.5 2.8 protein assembly complex, lipoprotein component (NCBI) compare
b3628 rfaB +0.5 4.2 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b4458 oxyS +0.5 1.5 global regulatory RNA (NCBI) compare
b1025 ycdT +0.5 1.5 predicted diguanylate cyclase (NCBI) compare
b2170 setB +0.5 2.4 lactose/glucose efflux system (NCBI) compare
b3119 tdcR +0.5 1.6 threonine dehydratase operon activator protein (VIMSS) compare
b1628 rsxB +0.5 2.6 electron transport complex protein RnfB (NCBI) compare
b4529 ydbJ +0.5 1.0 hypothetical protein (NCBI) compare
b2859 ygeO +0.5 1.2 hypothetical protein (RefSeq) compare
b1557 cspB +0.5 1.8 Qin prophage; cold shock protein (NCBI) compare
b4642 yoeD +0.5 1.8 no description compare
b3627 rfaI +0.5 3.4 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1919 yedO +0.5 2.1 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1728 ydjM +0.5 1.7 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1070 flgN +0.5 1.2 export chaperone for FlgK and FlgL (NCBI) compare
b0294 ykgK +0.5 2.2 predicted regulator (NCBI) compare
b3890 yiiF +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b2363 yfdT +0.5 1.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2948 yqgE +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b4552 yrhC +0.5 0.7 no description compare
b1186 nhaB +0.4 3.2 NhaB sodium/proton transporter (Katherine Huang) compare
b0661 miaB +0.4 3.9 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3658 selC +0.4 2.2 tRNA-Sec (NCBI) compare
b1837 yebW +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b2450 yffS +0.4 1.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b4050 yjbO +0.4 2.5 orf, hypothetical protein (VIMSS) compare
b1480 sra +0.4 1.3 30S ribosomal subunit protein S22 (NCBI) compare
b4012 yjaB +0.4 1.5 predicted acetyltransferase (NCBI) compare
b3387 dam +0.4 2.8 DNA adenine methylase (NCBI) compare
b1627 rsxA +0.4 2.1 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1233 ychJ +0.4 2.8 hypothetical protein (NCBI) compare
b3523 yhjE +0.4 3.6 predicted transporter (NCBI) compare
b1730 ydjO +0.4 0.7 predicted protein (RefSeq) compare
b1253 yciA +0.4 1.5 predicted hydrolase (NCBI) compare
b3816 corA +0.4 3.4 magnesium/nickel/cobalt transporter (NCBI) compare
b1258 yciF +0.4 0.7 hypothetical protein (NCBI) compare
b4453 ldrD +0.4 1.5 toxic polypeptide, small (NCBI) compare
b1194 ycgR +0.4 1.9 protein involved in flagellar function (NCBI) compare
b4222 ytfP +0.4 2.2 hypothetical protein (NCBI) compare
b4701 sokX +0.4 0.7 sok-related sRNA, function unknown (RefSeq) compare
b2101 yegW +0.4 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3753 rbsR +0.4 3.5 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b1685 ydiH +0.4 1.1 predicted protein (RefSeq) compare
b4071 nrfB +0.4 1.5 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1048 mdoG +0.4 2.5 glucan biosynthesis protein, periplasmic (NCBI) compare
b1823 cspC +0.4 1.3 stress protein, member of the CspA-family (NCBI) compare
b0933 ssuB +0.4 2.2 alkanesulfonate transporter subunit (NCBI) compare
b2642 yfjW +0.4 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2229 b2229 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b0240 crl +0.4 3.2 DNA-binding transcriptional regulator (NCBI) compare
b2759 ygcK +0.4 2.9 hypothetical protein (NCBI) compare
b3611 yibN +0.4 2.1 predicted rhodanese-related sulfurtransferase (NCBI) compare
b1335 ogt +0.4 1.7 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b3292 zntR +0.4 1.9 zinc-responsive transcriptional regulator (NCBI) compare
b1591 b1591 +0.4 0.9 putative oxidoreductase component (VIMSS) compare
b1548 nohA +0.4 1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b1236 galU +0.4 1.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3672 ivbL +0.4 1.4 ilvB operon leader peptide (NCBI) compare
b3500 gor +0.4 0.9 glutathione reductase (NCBI) compare
b2947 gshB +0.4 1.8 glutathione synthetase (NCBI) compare
b4571 wbbL +0.4 1.6 no description compare
b1743 spy +0.4 1.5 envelope stress induced periplasmic protein (NCBI) compare
b1877 yecT +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b4394 yjjX +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1761 gdhA +0.4 2.7 glutamate dehydrogenase (NCBI) compare
b1387 maoC +0.4 2.6 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b0754 aroG +0.4 2.7 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b0247 ykfG +0.4 0.9 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2204 napH +0.4 2.0 quinol dehydrogenase membrane component (NCBI) compare
b4181 yjfI +0.4 1.5 hypothetical protein (NCBI) compare
b3181 greA +0.4 2.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3101 yqjF +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b2937 speB +0.4 2.3 agmatinase (NCBI) compare
b2502 ppx +0.4 2.7 exopolyphosphatase (NCBI) compare
b1835 yebU +0.4 2.1 putative nucleolar proteins (VIMSS) compare
b1678 ynhG +0.4 2.9 hypothetical protein (NCBI) compare
b0982 yccY +0.4 2.0 putative phosphatase (VIMSS) compare
b2236 yfaE +0.4 1.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1244 oppB +0.4 2.2 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b1151 ymfO +0.3 2.0 e14 prophage; conserved protein (RefSeq) compare
b1196 ycgY +0.3 1.3 hypothetical protein (NCBI) compare
b1090 plsX +0.3 2.0 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1492 gadC +0.3 2.8 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b1642 slyA +0.3 1.3 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0296 ykgM +0.3 1.2 50S ribosomal protein L31 (NCBI) compare
b1363 trkG +0.3 0.4 Rac prophage; potassium transporter subunit (NCBI) compare
b3234 degQ +0.3 2.8 serine endoprotease, periplasmic (NCBI) compare
b3042 b3042 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0976 hyaE +0.3 2.1 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b4520 ymgF +0.3 0.8 hypothetical protein (NCBI) compare
b1132 hflD +0.3 2.3 hypothetical protein (NCBI) compare
b1287 yciW +0.3 1.7 putative oxidoreductase (VIMSS) compare
b0388 aroL +0.3 1.8 shikimate kinase II (NCBI) compare
b4503 yafF +0.3 0.6 no description compare
b0426 yajQ +0.3 2.6 orf, hypothetical protein (VIMSS) compare
b0617 citD +0.3 1.1 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1763 topB +0.3 1.9 DNA topoisomerase III (NCBI) compare
b1283 osmB +0.3 2.7 lipoprotein (NCBI) compare
b0212 gloB +0.3 1.6 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b0382 yaiB +0.3 2.8 hypothetical protein (NCBI) compare
b3739 atpI +0.3 2.3 membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS) compare
b4371 rsmC +0.3 2.8 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b3884 yihW +0.3 2.0 putative DEOR-type transcriptional regulator (VIMSS) compare
b4598 yncL +0.3 1.2 hypothetical protein (RefSeq) compare
b0759 galE +0.3 2.0 UDP-galactose-4-epimerase (VIMSS) compare
b4451 ryhB +0.3 1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2743 pcm +0.3 1.9 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b3446 yrhB +0.3 1.9 hypothetical protein (NCBI) compare
b4049 dusA +0.3 2.4 tRNA-dihydrouridine synthase A (RefSeq) compare
b2840 ygeA +0.3 2.3 predicted racemase (NCBI) compare
b2099 yegU +0.3 1.9 predicted hydrolase (NCBI) compare
b2783 chpR +0.3 1.4 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b0464 acrR +0.3 2.5 DNA-binding transcriptional repressor (NCBI) compare
b3071 yqjI +0.3 1.5 predicted transcriptional regulator (NCBI) compare
b0263 afuB +0.3 1.3 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b2934 cmtB +0.3 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1336 ydaH +0.3 2.0 putative pump protein (transport) (VIMSS) compare
b1295 ymjA +0.3 1.4 hypothetical protein (NCBI) compare
b3554 yiaF +0.3 2.7 orf, hypothetical protein (VIMSS) compare
b2435 amiA +0.3 2.1 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1869 yecN +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b0782 moaB +0.3 1.1 molybdopterin biosynthesis protein B (NCBI) compare
b1969 yedW +0.3 1.7 putative 2-component transcriptional regulator (VIMSS) compare
b0717 ybgP +0.3 2.0 predicted assembly protein (NCBI) compare
b0792 ybhR +0.3 1.9 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0864 artP +0.3 1.6 arginine transporter subunit (NCBI) compare
b1948 fliP +0.3 1.4 flagellar biosynthesis protein P (NCBI) compare
b2110 yehC +0.3 0.7 predicted periplasmic pilin chaperone (NCBI) compare
b3961 oxyR +0.3 2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2447 yffP +0.3 1.7 CPZ-55 prophage; predicted protein (NCBI) compare
b1518 lsrG +0.3 1.0 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1618 uidR +0.3 1.9 DNA-binding transcriptional repressor (NCBI) compare
b2455 eutE +0.3 1.4 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b1848 yebG +0.3 0.8 conserved protein regulated by LexA (NCBI) compare
b1334 fnr +0.3 1.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2195 ccmG +0.3 1.2 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b2300 yfcE +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b2156 lysP +0.3 2.2 lysine transporter (NCBI) compare
b2299 yfcD +0.3 1.6 predicted NUDIX hydrolase (NCBI) compare
b1306 pspC +0.3 1.5 DNA-binding transcriptional activator (NCBI) compare
b0280 yagN +0.3 1.9 CP4-6 prophage; predicted protein (NCBI) compare
b3516 gadX +0.3 2.6 DNA-binding transcriptional dual regulator (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Methotrexate in Escherichia coli BW25113

For stress Methotrexate across organisms