Experiment set52IT050 for Escherichia coli BW25113

Compare to:

Carbon source D-Glucose 10 mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +2.0 10.8 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +1.8 17.1 glycine dehydrogenase (NCBI) compare
b2905 gcvT +1.6 11.9 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2808 gcvA +1.6 9.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0059 hepA +1.2 11.2 ATP-dependent helicase HepA (NCBI) compare
b3977 tyrU +1.2 2.2 tRNA-Tyr (NCBI) compare
b4203 rplI +1.1 6.3 50S ribosomal protein L9 (NCBI) compare
b2829 ptsP +1.0 8.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3206 npr +1.0 5.8 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4546 ypeB +1.0 2.1 hypothetical protein (NCBI) compare
b3164 pnp +0.9 3.0 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2951 yggS +0.9 5.2 predicted enzyme (NCBI) compare
b2688 gshA +0.8 6.5 glutamate--cysteine ligase (NCBI) compare
b2114 metG +0.8 2.2 methionyl-tRNA synthetase (NCBI) compare
b1161 ycgX +0.8 0.8 hypothetical protein (NCBI) compare
b1222 narX +0.8 4.1 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b2236 yfaE +0.8 3.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4568 ytjA +0.7 2.3 predicted protein (RefSeq) compare
b1438 ydcQ +0.7 3.6 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1514 lsrC +0.7 2.2 AI2 transporter (NCBI) compare
b1486 ddpB +0.7 1.6 D-ala-D-ala transporter subunit (NCBI) compare
b4598 yncL +0.7 2.0 hypothetical protein (RefSeq) compare
b1259 yciG +0.7 1.8 orf, hypothetical protein (VIMSS) compare
b2953 yggU +0.6 2.8 orf, hypothetical protein (VIMSS) compare
b1823 cspC +0.6 2.4 stress protein, member of the CspA-family (NCBI) compare
b4647 mokA +0.6 0.8 no description compare
b3842 rfaH +0.6 4.4 transcriptional activator RfaH (NCBI) compare
b1591 b1591 +0.6 2.1 putative oxidoreductase component (VIMSS) compare
b2948 yqgE +0.6 3.2 orf, hypothetical protein (VIMSS) compare
b3643 rph +0.6 1.8 ribonuclease PH (NCBI) compare
b1095 fabF +0.6 4.1 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1618 uidR +0.6 2.7 DNA-binding transcriptional repressor (NCBI) compare
b3630 rfaP +0.6 5.1 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0759 galE +0.6 3.7 UDP-galactose-4-epimerase (VIMSS) compare
b3627 rfaI +0.6 4.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b4294 insA-7 +0.6 2.3 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2356 yfdM +0.6 1.0 orf, hypothetical protein (VIMSS) compare
b4589 ylcI +0.6 2.7 hypothetical protein (RefSeq) compare
b1150 ymfR +0.6 1.0 e14 prophage; predicted protein (NCBI) compare
b1685 ydiH +0.6 1.3 predicted protein (RefSeq) compare
b1656 sodB +0.6 2.7 superoxide dismutase, Fe (NCBI) compare
b1752 ydjZ +0.6 1.8 conserved inner membrane protein (NCBI) compare
b3620 rfaF +0.6 3.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0178 hlpA +0.5 2.5 periplasmic chaperone (NCBI) compare
b2947 gshB +0.5 2.2 glutathione synthetase (NCBI) compare
b1961 dcm +0.5 1.0 DNA cytosine methylase (NCBI) compare
b3261 fis +0.5 1.8 DNA-binding protein Fis (NCBI) compare
b1382 ynbE +0.5 1.2 predicted lipoprotein (NCBI) compare
b4141 yjeH +0.5 4.2 predicted transporter (NCBI) compare
b1589 ynfG +0.5 1.9 oxidoreductase, Fe-S subunit (NCBI) compare
b0784 moaD +0.5 2.3 molybdopterin synthase, small subunit (NCBI) compare
b1818 manY +0.5 1.9 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0051 ksgA +0.5 2.8 dimethyladenosine transferase (NCBI) compare
b2136 yohD +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b3806 cyaA +0.5 2.9 adenylate cyclase (NCBI) compare
b0550 rusA +0.5 2.1 DLP12 prophage; endonuclease RUS (NCBI) compare
b4369 leuP +0.5 1.3 tRNA-Leu (NCBI) compare
b0852 rimK +0.5 2.7 ribosomal protein S6 modification protein (NCBI) compare
b4453 ldrD +0.5 1.6 toxic polypeptide, small (NCBI) compare
b3889 yiiE +0.5 1.9 predicted transcriptional regulator (RefSeq) compare
b2990 hybG +0.5 0.9 hydrogenase 2 accessory protein (NCBI) compare
b1627 rsxA +0.5 2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b4000 hupA +0.5 2.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3119 tdcR +0.5 2.3 threonine dehydratase operon activator protein (VIMSS) compare
b2804 fucU +0.5 1.8 L-fucose mutarotase (NCBI) compare
b2572 rseA +0.5 2.2 anti-sigma factor (NCBI) compare
b3632 rfaQ +0.5 4.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1141 ymfG +0.5 1.9 e14 prophage; predicted excisionase (NCBI) compare
b2435 amiA +0.5 2.2 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b3979 thrT +0.4 0.4 tRNA-Thr (NCBI) compare
b3631 rfaG +0.4 4.1 glucosyltransferase I (NCBI) compare
b3928 yiiU +0.4 3.0 hypothetical protein (NCBI) compare
b1760 ynjH +0.4 2.6 hypothetical protein (NCBI) compare
b3264 envR +0.4 2.3 DNA-binding transcriptional regulator (NCBI) compare
b3052 rfaE +0.4 2.7 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b4202 rpsR +0.4 1.0 30S ribosomal protein S18 (NCBI) compare
b1634 tppB +0.4 2.6 putative tripeptide transporter permease (NCBI) compare
b4041 plsB +0.4 1.4 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0503 ybbB +0.4 2.5 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1936 intG +0.4 0.6 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1667 ydhR +0.4 1.2 hypothetical protein (NCBI) compare
b2910 zapA +0.4 2.5 protein that localizes to the cytokinetic ring (NCBI) compare
b1855 lpxM +0.4 2.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1613 manA +0.4 1.2 mannose-6-phosphate isomerase (VIMSS) compare
b3068 ygjF +0.4 1.5 G/U mismatch-specific DNA glycosylase (NCBI) compare
b3658 selC +0.4 2.1 tRNA-Sec (NCBI) compare
b0751 pnuC +0.4 3.0 predicted nicotinamide mononucleotide transporter (NCBI) compare
b4701 sokX +0.4 0.4 sok-related sRNA, function unknown (RefSeq) compare
b4012 yjaB +0.4 1.8 predicted acetyltransferase (NCBI) compare
b4461 yfjD +0.4 3.3 predicted inner membrane protein (RefSeq) compare
b1335 ogt +0.4 1.4 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b2111 yehD +0.4 1.9 predicted fimbrial-like adhesin protein (NCBI) compare
b2494 yfgC +0.4 3.0 predicted peptidase (NCBI) compare
b1066 rimJ +0.4 2.4 ribosomal-protein-S5-alanine N-acetyltransferase (NCBI) compare
b2058 wcaB +0.4 0.8 predicted acyl transferase (NCBI) compare
b1160 elbA +0.4 0.6 hypothetical protein (NCBI) compare
b1394 paaG +0.4 1.3 enoyl-CoA hydratase (NCBI) compare
b3621 rfaC +0.4 2.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2939 yqgB +0.4 0.8 predicted protein (RefSeq) compare
b1802 yeaW +0.4 1.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1633 nth +0.4 2.2 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b0926 ycbK +0.4 2.1 hypothetical protein (NCBI) compare
b3778 rep +0.4 2.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3387 dam +0.4 3.2 DNA adenine methylase (NCBI) compare
b2421 cysM +0.4 1.6 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1739 osmE +0.4 1.1 DNA-binding transcriptional activator (NCBI) compare
b3181 greA +0.4 2.5 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1789 yeaL +0.4 1.6 conserved inner membrane protein (NCBI) compare
b2038 rfbC +0.4 2.5 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b1441 ydcT +0.4 1.5 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1610 tus +0.4 2.3 DNA replication terminus site-binding protein (NCBI) compare
b3129 sohA +0.4 1.4 predicted regulator (NCBI) compare
b0949 uup +0.4 2.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4451 ryhB +0.4 0.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0623 cspE +0.4 1.0 cold shock protein E (NCBI) compare
b0661 miaB +0.4 3.0 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1253 yciA +0.4 1.5 predicted hydrolase (NCBI) compare
b1640 ydhH +0.4 1.5 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b3350 kefB +0.4 3.3 glutathione-regulated potassium-efflux system protein (NCBI) compare
b3292 zntR +0.4 1.7 zinc-responsive transcriptional regulator (NCBI) compare
b1380 ldhA +0.4 0.8 D-lactate dehydrogenase (NCBI) compare
b1976 b1976 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b2684 mprA +0.3 2.0 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1333 uspE +0.3 1.9 stress-induced protein (NCBI) compare
b1573 ydfC +0.3 0.8 Qin prophage; predicted protein (RefSeq) compare
b1914 uvrY +0.3 1.7 response regulator (NCBI) compare
b2003 yeeT +0.3 2.1 CP4-44 prophage; predicted protein (NCBI) compare
b2366 dsdA +0.3 1.6 D-serine dehydratase (NCBI) compare
b4056 yjbQ +0.3 2.5 hypothetical protein (NCBI) compare
b0882 clpA +0.3 2.8 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b1630 rsxD +0.3 1.8 electron transport complex protein RnfD (NCBI) compare
b1918 yecS +0.3 1.8 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4360 yjjA +0.3 1.2 putative glycoprotein/receptor (VIMSS) compare
b3890 yiiF +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1904 yecR +0.3 0.8 hypothetical protein (NCBI) compare
b3034 nudF +0.3 1.9 ADP-ribose pyrophosphatase (NCBI) compare
b1111 ycfQ +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0219 yafV +0.3 1.5 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b0381 ddlA +0.3 2.5 D-alanylalanine synthetase (NCBI) compare
b1949 fliQ +0.3 1.3 flagellar biosynthesis protein Q (NCBI) compare
b4502 yeiW +0.3 1.7 hypothetical protein (NCBI) compare
b0615 citF +0.3 2.1 citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI) compare
b3024 ygiW +0.3 1.8 hypothetical protein (NCBI) compare
b1013 ycdC +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1891 flhC +0.3 1.1 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b2620 smpB +0.3 0.6 SsrA-binding protein (NCBI) compare
b1977 asnT +0.3 1.4 tRNA-Asn (NCBI) compare
b1423 ydcJ +0.3 1.1 hypothetical protein (NCBI) compare
b0190 yaeQ +0.3 2.2 hypothetical protein (NCBI) compare
b2618 b2618 +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b0560 nohB +0.3 1.0 DLP12 prophage; DNA packaging protein (NCBI) compare
b1143 ymfI +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b4227 ytfQ +0.3 2.3 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1147 ymfL +0.3 1.4 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b4698 mgrR +0.3 0.8 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1609 rstB +0.3 1.4 sensory histidine kinase in two-component regulatory system with RstA (NCBI) compare
b2314 dedD +0.3 1.4 putative lipoprotein (VIMSS) compare
b1629 rsxC +0.3 2.2 electron transport complex protein RnfC (NCBI) compare
b2531 iscR +0.3 0.6 DNA-binding transcriptional repressor (NCBI) compare
b0955 ycbZ +0.3 2.5 predicted peptidase (NCBI) compare
b3033 yqiB +0.3 2.1 predicted dehydrogenase (NCBI) compare
b1478 adhP +0.3 2.0 alcohol dehydrogenase (VIMSS) compare
b4406 yaeP +0.3 0.8 hypothetical protein (NCBI) compare
b0502 ylbG +0.3 1.3 no description compare
b2099 yegU +0.3 1.7 predicted hydrolase (NCBI) compare
b2735 ygbI +0.3 1.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0677 nagA +0.3 1.9 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b4376 osmY +0.3 2.4 periplasmic protein (NCBI) compare
b1461 ydcE +0.3 1.1 4-oxalocrotonate tautomerase (NCBI) compare
b4676 yoaK +0.3 0.3 expressed protein (RefSeq) compare
b3929 rraA +0.3 2.1 ribonuclease activity regulator protein RraA (NCBI) compare
b3423 glpR +0.3 2.3 DNA-binding transcriptional repressor (NCBI) compare
b2444 yffM +0.3 1.0 CPZ-55 prophage; predicted protein (NCBI) compare
b1562 hokD +0.3 1.0 Qin prophage; small toxic polypeptide (NCBI) compare
b1621 malX +0.3 1.7 fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI) compare
b2141 yohJ +0.3 1.1 hypothetical protein (NCBI) compare
b1790 yeaM +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2646 ypjF +0.3 1.1 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b4426 isrA +0.3 1.1 unknown RNA (NCBI) compare
b0234 yafP +0.3 0.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1688 ydiK +0.3 1.7 predicted inner membrane protein (NCBI) compare
b3628 rfaB +0.3 2.4 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b4128 yjdK +0.3 0.3 hypothetical protein (NCBI) compare
b1406 ydbC +0.3 1.8 predicted oxidoreductase, NAD(P)-binding (NCBI) compare
b1548 nohA +0.3 0.6 Qin prophage; predicted packaging protein (RefSeq) compare
b0866 ybjQ +0.3 0.6 hypothetical protein (NCBI) compare
b3644 yicC +0.3 1.6 hypothetical protein (NCBI) compare
b0610 rnk +0.3 1.2 nucleoside diphosphate kinase regulator (NCBI) compare
b4093 phnO +0.3 1.0 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4135 yjdC +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b4384 deoD +0.3 2.2 purine nucleoside phosphorylase (NCBI) compare
b0247 ykfG +0.3 0.6 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1540 ydfH +0.3 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b4620 yjbT +0.3 1.5 hypothetical protein, no homologs (RefSeq) compare
b0295 ykgL +0.3 1.7 hypothetical protein (NCBI) compare
b2082 ogrK +0.3 1.4 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b1794 yeaP +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b3780 rhlB +0.3 2.2 ATP-dependent RNA helicase (NCBI) compare
b3443 yrhA +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b0836 yliH +0.3 1.4 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in carbon source experiments

For carbon source D-Glucose across organisms