Experiment set4S85 for Pseudomonas sp. RS175

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Sodium propionate carbon source 20 mM

Group: carbon source
Media: MME_noCarbon + Sodium propionate (20 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Sodium propionate in Pseudomonas sp. RS175

For carbon source Sodium propionate across organisms

SEED Subsystems

Subsystem #Specific
Anaerobic respiratory reductases 1
Fermentations: Mixed acid 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
partial TCA cycle (obligate autotrophs) 8 8 1
nitrogen remobilization from senescing leaves 8 5 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 1
formaldehyde assimilation I (serine pathway) 13 6 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
mixed acid fermentation 16 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
ethene biosynthesis V (engineered) 25 19 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 20 1