Experiment set4S77 for Pseudomonas sp. RS175

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Shikimic Acid carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + Shikimic Acid (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Shikimic Acid in Pseudomonas sp. RS175

For carbon source Shikimic Acid across organisms

SEED Subsystems

Subsystem #Specific
Chorismate Synthesis 2
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 2
Alginate metabolism 1
Cinnamic Acid Degradation 1
D-Galacturonate and D-Glucuronate Utilization 1
Gentisare degradation 1
Phenylpropanoid compound degradation 1
Quinate degradation 1
Salicylate and gentisate catabolism 1
p-Hydroxybenzoate degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
shikimate degradation II 2 1 1
chorismate biosynthesis from 3-dehydroquinate 5 5 2
gallate biosynthesis 3 2 1
quinate degradation I 3 1 1
quinate degradation II 3 1 1
chorismate biosynthesis I 7 7 2
superpathway of L-phenylalanine biosynthesis 10 10 2
superpathway of L-tyrosine biosynthesis 10 10 2
chorismate biosynthesis II (archaea) 12 8 2
superpathway of L-tryptophan biosynthesis 13 13 2
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of chorismate metabolism 59 43 2
superpathway of aromatic compound degradation via 3-oxoadipate 35 13 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 10 1