Experiment set4S75 for Pseudomonas sp. RS175
Sodium D,L-Lactate carbon source 20 mM
Group: carbon sourceMedia: MME_noCarbon + Sodium D,L-Lactate (20 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 5 genes in this experiment
For carbon source Sodium D,L-Lactate in Pseudomonas sp. RS175
For carbon source Sodium D,L-Lactate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Lactate utilization | 5 |
| L-rhamnose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyruvate fermentation to (S)-lactate | 1 | 1 | 1 |
| pyruvate fermentation to acetate and (S)-lactate I | 4 | 4 | 1 |
| pyruvate fermentation to acetate and lactate II | 4 | 3 | 1 |
| homolactic fermentation | 12 | 10 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 5 | 1 |
| Bifidobacterium shunt | 15 | 15 | 1 |
| superpathway of glucose and xylose degradation | 17 | 17 | 1 |
| heterolactic fermentation | 18 | 16 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 15 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 14 | 1 |