Experiment set4S71 for Pseudomonas sp. RS175
D-Glucosamine Hydrochloride carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + D-Glucosamine Hydrochloride (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 12 genes in this experiment
For carbon source D-Glucosamine Hydrochloride in Pseudomonas sp. RS175
For carbon source D-Glucosamine Hydrochloride across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-serine degradation | 3 | 3 | 3 |
| L-serine degradation | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| L-tryptophan degradation II (via pyruvate) | 3 | 2 | 2 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| glycine betaine degradation III | 7 | 7 | 2 |
| glycine betaine degradation I | 8 | 6 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| chorismate biosynthesis from 3-dehydroquinate | 5 | 5 | 1 |
| chorismate biosynthesis I | 7 | 7 | 1 |
| pyrroloquinoline quinone biosynthesis | 7 | 4 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 2 |
| chorismate biosynthesis II (archaea) | 12 | 8 | 1 |
| superpathway of L-tryptophan biosynthesis | 13 | 13 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| superpathway of chorismate metabolism | 59 | 43 | 1 |