Experiment set4S66 for Pseudomonas sp. RS175

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D-(+)-Galactosamine hydrochloride carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + D-(+)-Galactosamine hydrochloride (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 10 genes in this experiment

For carbon source D-(+)-Galactosamine hydrochloride in Pseudomonas sp. RS175

For carbon source D-(+)-Galactosamine hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
D-galactonate catabolism 2
Flagellar motility 1
Flagellum 1
L-Arabinose utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-serine degradation 3 3 3
L-serine degradation 3 3 2
D-galactonate degradation 3 3 2
L-cysteine degradation II 3 2 2
L-tryptophan degradation II (via pyruvate) 3 2 2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
L-methionine biosynthesis II 6 5 2
glycine betaine degradation III 7 7 2
L-glucose degradation 7 3 2
glycine betaine degradation I 8 6 2
L-mimosine degradation 8 4 2
glutathione-mediated detoxification I 8 3 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
purine nucleobases degradation II (anaerobic) 24 16 2