Experiment set4S615 for Rhodanobacter sp. FW510-T8

Compare to:

R2A_PIPES with Metal_ion_mix_sitestressplate 2 x

Group: stress
Media: R2A_PIPES + 2x Metal_ion_mix_sitestressplate, pH=7
Culturing: rhodanobacter_T8_ML1, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hira on 10/21/2025
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt

Metal_ion_mix_sitestressplate 2x includes: 0.016 mM Cobalt chloride hexahydrate, 0.004 mM Cadmium chloride hemipentahydrate, 0.106 mM Nickel (II) chloride hexahydrate, 2.5 mM Aluminum chloride hydrate, 2 mM Manganese (II) chloride tetrahydrate, 0.02 mM Zinc chloride (final concentrations)

Specific Phenotypes

For 2 genes in this experiment

For stress Metal_ion_mix_sitestressplate in Rhodanobacter sp. FW510-T8

For stress Metal_ion_mix_sitestressplate across organisms

SEED Subsystems

Subsystem #Specific
High affinity phosphate transporter and control of PHO regulon 1
Phosphate metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.