Experiment set4S61 for Pseudomonas sp. RS175
Glycerol carbon source 20 mM
Group: carbon sourceMedia: MME_noCarbon + Glycerol (20 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source Glycerol in Pseudomonas sp. RS175
For carbon source Glycerol across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycerol degradation I | 3 | 3 | 2 |
| glycerol and glycerophosphodiester degradation | 4 | 4 | 2 |
| arsenate detoxification III | 2 | 2 | 1 |
| phospholipid remodeling (phosphatidylethanolamine, yeast) | 4 | 2 | 1 |
| arsenic detoxification (plants) | 6 | 5 | 1 |
| arsenic detoxification (yeast) | 12 | 4 | 1 |
| arsenic detoxification (mammals) | 17 | 8 | 1 |