Experiment set4S59 for Pseudomonas sp. RS175
L-pyroglutamic acid carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + L-pyroglutamic acid (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source L-pyroglutamic acid in Pseudomonas sp. RS175
For carbon source L-pyroglutamic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alginate metabolism | 1 |
| Cyanate hydrolysis | 1 |
| DNA-binding regulatory proteins, strays | 1 |
| Mannose Metabolism | 1 |
| Sialic Acid Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation II | 2 | 1 | 1 |
| D-mannose degradation I | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 2 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |