Experiment set4S41 for Pseudomonas sp. RS175
L-Proline carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + L-Proline (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 1 genes in this experiment
For carbon source L-Proline in Pseudomonas sp. RS175
For carbon source L-Proline across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Arginine and Ornithine Degradation | 1 |
| Proline, 4-hydroxyproline uptake and utilization | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-proline degradation I | 3 | 3 | 2 |
| L-arginine degradation I (arginase pathway) | 3 | 2 | 1 |
| ethene biosynthesis II (microbes) | 4 | 1 | 1 |
| (5R)-carbapenem carboxylate biosynthesis | 6 | 1 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 5 | 1 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |