Experiment set4S34 for Pseudomonas sp. RS175

D-Mannitol carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + D-Mannitol (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 8 genes in this experiment

For carbon source D-Mannitol in Pseudomonas sp. RS175

For carbon source D-Mannitol across organisms

SEED Subsystems

Subsystem	#Specific
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	8
Mannitol Utilization	2
Fructose utilization	1
Sucrose utilization	1
Sucrose utilization Shewanella	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism
• C21-Steroid hormone metabolism
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• Starch and sucrose metabolism
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-xylose degradation I	2	2	1
D-arabinitol degradation I	2	1	1
xylitol degradation I	2	1	1
mannitol cycle	5	4	2
D-sorbitol degradation I	3	2	1
sucrose degradation I (sucrose phosphotransferase)	3	1	1
sucrose degradation IV (sucrose phosphorylase)	4	3	1
sucrose degradation III (sucrose invertase)	4	3	1
D-fructuronate degradation	4	3	1
sucrose degradation VII (sucrose 3-dehydrogenase)	4	1	1
sucrose degradation II (sucrose synthase)	5	4	1
superpathway of β-D-glucuronosides degradation	7	4	1
superpathway of hexuronide and hexuronate degradation	10	4	1
superpathway of glucose and xylose degradation	17	17	1
heterolactic fermentation	18	16	1
superpathway of anaerobic sucrose degradation	19	15	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	14	1
superpathway of pentose and pentitol degradation	42	14	1