Experiment set4S310 for Escherichia coli ECRC101
RB68
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 25 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 6/9/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 5 genes in this experiment
For phage Phage in Escherichia coli ECRC101
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Multidrug Resistance Efflux Pumps | 1 |
| Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| S-methyl-5'-thioadenosine degradation II | 1 | 1 | 1 |
| adenine and adenosine salvage III | 4 | 4 | 3 |
| adenine and adenosine salvage V | 3 | 3 | 2 |
| purine ribonucleosides degradation | 6 | 6 | 3 |
| adenine and adenosine salvage I | 2 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 2 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| L-methionine salvage cycle III | 11 | 5 | 1 |
| superpathway of purine nucleotide salvage | 14 | 13 | 1 |