Experiment set4S29 for Pseudomonas sp. RS175

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2-Keto-D-gluconic acid hemicalcium salt monohydrate carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + 2-Keto-D-gluconic acid hemicalcium salt monohydrate (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 11 genes in this experiment

For carbon source 2-Keto-D-gluconic acid hemicalcium salt monohydrate in Pseudomonas sp. RS175

For carbon source 2-Keto-D-gluconic acid hemicalcium salt monohydrate across organisms

SEED Subsystems

Subsystem #Specific
2-Ketogluconate Utilization 4
Fatty Acid Biosynthesis FASII 1
Phosphate metabolism 1
ZZ gjo need homes 1
dTDP-rhamnose synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acyl carrier protein metabolism 2 2 1
glucose degradation (oxidative) 5 4 2
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis 3 3 1
CDP-6-deoxy-D-gulose biosynthesis 5 3 1
NAD(P)/NADPH interconversion 6 3 1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis 8 3 1
streptomycin biosynthesis 18 3 1