Experiment set4S28 for Pseudomonas sp. RS175
2-Keto-D-gluconic acid hemicalcium salt monohydrate carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + 2-Keto-D-gluconic acid hemicalcium salt monohydrate (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 12 genes in this experiment
For carbon source 2-Keto-D-gluconic acid hemicalcium salt monohydrate in Pseudomonas sp. RS175
For carbon source 2-Keto-D-gluconic acid hemicalcium salt monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| 2-Ketogluconate Utilization | 4 |
| Fatty Acid Biosynthesis FASII | 1 |
| ZZ gjo need homes | 1 |
| dTDP-rhamnose synthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Glutathione metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Pantothenate and CoA biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: