Experiment set4S22 for Pseudomonas sp. RS175
D-Ribose carbon source 10 mM
Group: carbon sourceMedia: MME_noCarbon + D-Ribose (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 4 genes in this experiment
For carbon source D-Ribose in Pseudomonas sp. RS175
For carbon source D-Ribose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-galactonate catabolism | 2 |
| Coenzyme PQQ synthesis | 1 |
| Pyrroloquinoline Quinone biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-galactonate degradation | 3 | 3 | 2 |
| L-lyxonate degradation | 3 | 2 | 1 |
| L-glucose degradation | 7 | 3 | 2 |
| L-arabinose degradation III | 6 | 5 | 1 |
| pyrroloquinoline quinone biosynthesis | 7 | 4 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 14 | 1 |