Experiment set4S201 for Pseudomonas aeruginosa MRSN321

0.0001311019372 M Ceftazidime hydrate

Group: stress
Media: LB + Ceftazidime hydrate (0.000131 M), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 8/12/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 32 genes in this experiment

For stress Ceftazidime hydrate in Pseudomonas aeruginosa MRSN321

For stress Ceftazidime hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Peptidoglycan Biosynthesis	3
Lipid A modifications	2
Bacterial Cytoskeleton	1
Beta-lactamase	1
DNA repair, UvrABC system	1
Dissimilatory nitrite reductase	1
Heme biosynthesis orphans	1
Methionine Salvage	1
Ribosome biogenesis bacterial	1
Tn552	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Urea cycle and metabolism of amino groups
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Lysine degradation
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Biotin metabolism
• Diterpenoid biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-methyl-5'-thioadenosine degradation II	1	1	1
S-methyl-5'-thioadenosine degradation III	2	1	1
5'-deoxyadenosine degradation I	3	2	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	3
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	3
peptidoglycan biosynthesis II (staphylococci)	17	12	3
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan recycling II	10	9	1
L-methionine salvage cycle III	11	9	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan recycling I	14	12	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1