Experiment set4S199 for Pseudomonas aeruginosa MRSN321

0.0009703712996 M Phosphomycin disodium salt

Group: stress
Media: LB + Phosphomycin disodium salt (0.00097 M), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 8/12/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 43 genes in this experiment

For stress Phosphomycin disodium salt in Pseudomonas aeruginosa MRSN321

For stress Phosphomycin disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	3
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Peptidoglycan Biosynthesis	2
ZZ gjo need homes	2
Alginate metabolism	1
Alkylphosphonate utilization	1
Central meta-cleavage pathway of aromatic compound degradation	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
DNA repair, bacterial	1
De Novo Pyrimidine Synthesis	1
Folate Biosynthesis	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Histidine Degradation	1
LMPTP YwlE cluster	1
MLST	1
Molybdenum cofactor biosynthesis	1
NAD and NADP cofactor biosynthesis global	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Pseudomonas quinolone signal PQS	1
Pterin biosynthesis	1
Purine conversions	1
Pyrimidine utilization	1
Queuosine-Archaeosine Biosynthesis	1
Quorum sensing regulation in Pseudomonas	1
Rhamnolipids in Pseudomonas	1
Thioredoxin-disulfide reductase	1
Transcription factors bacterial	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Purine metabolism
• Fructose and mannose metabolism
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycerophospholipid metabolism
• Folate biosynthesis
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Lysine degradation
• Glutathione metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Keratan sulfate biosynthesis
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Biotin metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Drug metabolism - other enzymes

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphatidylcholine biosynthesis VI	1	1	1
pyrimidine nucleobases salvage I	1	1	1
glycerol degradation I	3	3	2
glycerol and glycerophosphodiester degradation	4	4	2
pyrimidine nucleobases salvage II	2	2	1
glycerol-3-phosphate shuttle	2	2	1
xanthine and xanthosine salvage	2	2	1
guanine and guanosine salvage I	2	2	1
glycerophosphodiester degradation	2	2	1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer	2	2	1
guanine and guanosine salvage II	2	2	1
nitrate reduction IX (dissimilatory)	2	1	1
glycerol-3-phosphate to hydrogen peroxide electron transport	2	1	1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer	2	1	1
glycerol-3-phosphate to fumarate electron transfer	2	1	1
rhamnolipid biosynthesis	3	3	1
prenylated FMNH2 biosynthesis	3	2	1
adenine salvage	3	2	1
6-hydroxymethyl-dihydropterin diphosphate biosynthesis IV (Plasmodium)	3	2	1
sn-glycerol 3-phosphate anaerobic respiration	3	2	1
superpathway of guanine and guanosine salvage	3	2	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
adenine and adenosine salvage III	4	4	1
superpathway of pyrimidine nucleobases salvage	4	4	1
tetrahydromonapterin biosynthesis	4	3	1
threo-tetrahydrobiopterin biosynthesis	4	2	1
preQ0 biosynthesis	4	2	1
erythro-tetrahydrobiopterin biosynthesis I	4	2	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	3
creatinine degradation I	4	1	1
6-hydroxymethyl-dihydropterin diphosphate biosynthesis I	5	4	1
creatinine degradation II	5	1	1
peptidoglycan biosynthesis II (staphylococci)	17	12	3
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	3
drosopterin and aurodrosopterin biosynthesis	7	5	1
alginate degradation	7	1	1
glycine betaine degradation I	8	6	1
2-heptyl-3-hydroxy-4(1H)-quinolone biosynthesis	8	3	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan recycling II	10	9	1
superpathway of tetrahydrofolate biosynthesis	10	9	1
superpathway of pyrimidine ribonucleosides salvage	10	6	1
superpathway of quinolone and alkylquinolone biosynthesis	10	5	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	11	1
superpathway of purine nucleotide salvage	14	13	1
peptidoglycan recycling I	14	12	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
superpathway of chorismate metabolism	59	45	1