Experiment set4S19 for Pseudomonas sp. RS175

Compare to:

D-Glucuronic acid sodium salt monohydrate carbon source 10 mM

Group: carbon source
Media: MME_noCarbon + D-Glucuronic acid sodium salt monohydrate (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-January-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 10 genes in this experiment

For carbon source D-Glucuronic acid sodium salt monohydrate in Pseudomonas sp. RS175

For carbon source D-Glucuronic acid sodium salt monohydrate across organisms

SEED Subsystems

Subsystem #Specific
D-galactarate, D-glucarate and D-glycerate catabolism 2
D-Galacturonate and D-Glucuronate Utilization 1
Entner-Doudoroff Pathway 1
L-Arabinose utilization 1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway 1
Lactose and Galactose Uptake and Utilization 1
N-linked Glycosylation in Bacteria 1
Rhamnose containing glycans 1
Xylose utilization 1
ZZ gjo need homes 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-glucarate degradation II 3 3 3
UDP-α-D-galactose biosynthesis 1 1 1
UDP-N-acetyl-D-galactosamine biosynthesis I 1 1 1
D-galactarate degradation II 3 3 2
D-glucuronate degradation II 5 4 3
D-galacturonate degradation II 5 3 3
D-glucuronate degradation III 2 2 1
UDP-α-D-galactofuranose biosynthesis 2 1 1
D-galacturonate degradation III 2 1 1
ethanol degradation II 3 3 1
ethanol degradation IV 3 3 1
hypotaurine degradation 3 2 1
sorbitol biosynthesis II 3 2 1
L-lyxonate degradation 3 2 1
ethanol degradation III 3 2 1
D-galactose detoxification 3 1 1
histamine degradation 3 1 1
trans-4-hydroxy-L-proline degradation II 4 4 1
D-glucarate degradation I 4 3 1
putrescine degradation III 4 3 1
phytol degradation 4 3 1
fatty acid α-oxidation I (plants) 4 2 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 2 1
L-ascorbate biosynthesis VI (plants, myo-inositol pathway) 4 1 1
mitochondrial NADPH production (yeast) 5 4 1
superpathway of D-glucarate and D-galactarate degradation 5 4 1
octane oxidation 5 4 1
glucose degradation (oxidative) 5 4 1
glucose and glucose-1-phosphate degradation 5 4 1
D-galactose degradation I (Leloir pathway) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
dopamine degradation 5 2 1
D-xylose degradation III 5 1 1
D-xylose degradation V 5 1 1
L-arabinose degradation III 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) 6 3 1
3-methyl-branched fatty acid α-oxidation 6 3 1
D-arabinose degradation III 6 2 1
alkane oxidation 6 1 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 14 5
noradrenaline and adrenaline degradation 13 8 2
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
serotonin degradation 7 4 1
L-ascorbate biosynthesis VIII (engineered pathway) 7 2 1
ceramide degradation by α-oxidation 7 2 1
stachyose degradation 7 2 1
limonene degradation IV (anaerobic) 7 1 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 7 1
aromatic biogenic amine degradation (bacteria) 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
Entner-Doudoroff pathway III (semi-phosphorylative) 9 7 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 6 1
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
UDP-sugars interconversion 9 2 1
colanic acid building blocks biosynthesis 11 9 1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 18 1 1
superpathway of pentose and pentitol degradation 42 14 2
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1