Experiment set4IT094 for Pseudomonas simiae WCS417
LB with Polymyxin B sulfate 0.006 mg/ml
Group: stressMedia: LB + Polymyxin B sulfate (0.006 mg/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1164 C7,C8
Specific Phenotypes
For 8 genes in this experiment
For stress Polymyxin B sulfate in Pseudomonas simiae WCS417
For stress Polymyxin B sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Multidrug Resistance Efflux Pumps | 1 |
| Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) | 1 |
| N-linked Glycosylation in Bacteria | 1 |
| Orphan regulatory proteins | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Puromycin biosynthesis
- Tyrosine metabolism
- Benzoate degradation via hydroxylation
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Limonene and pinene degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-N-acetyl-β-L-fucosamine biosynthesis | 4 | 2 | 1 |
| UDP-N-acetyl-β-L-quinovosamine biosynthesis | 4 | 1 | 1 |
| CMP-pseudaminate biosynthesis | 6 | 1 | 1 |
| CMP-diacetamido-8-epilegionaminic acid biosynthesis | 7 | 1 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 9 | 1 |