Experiment set4IT091 for Escherichia coli BW25113

LB with octanol 0.08 vol%

Group: stress
Media: LB + octanol (0.08 vol%)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Mitch on 3/21/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 27 genes in this experiment

For stress octanol in Escherichia coli BW25113

For stress octanol across organisms

SEED Subsystems

Subsystem	#Specific
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Selenocysteine metabolism	2
ATP-dependent RNA helicases, bacterial	1
Biotin biosynthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
KDO2-Lipid A biosynthesis	1
LOS core oligosaccharide biosynthesis	1
Proline, 4-hydroxyproline uptake and utilization	1
Rhamnose containing glycans	1
Transcription initiation, bacterial sigma factors	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1
n-Phenylalkanoic acid degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Fatty acid metabolism
• Selenoamino acid metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Aminoacyl-tRNA biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
S-methyl-5'-thioadenosine degradation II	1	1	1
adenine and adenosine salvage III	4	4	3
adenine and adenosine salvage V	3	3	2
purine ribonucleosides degradation	6	6	3
adenine and adenosine salvage I	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
cinnamoyl-CoA biosynthesis	2	1	1
γ-linolenate biosynthesis II (animals)	2	1	1
linoleate biosynthesis II (animals)	2	1	1
adenosine nucleotides degradation II	5	5	2
L-selenocysteine biosynthesis I (bacteria)	3	3	1
3-methyl-branched fatty acid α-oxidation	6	3	2
oleate biosynthesis I (plants)	3	1	1
alkane biosynthesis II	3	1	1
dTDP-N-acetylthomosamine biosynthesis	4	4	1
phytol degradation	4	3	1
dTDP-N-acetylviosamine biosynthesis	4	2	1
dTDP-β-D-fucofuranose biosynthesis	4	2	1
dTDP-6-deoxy-α-D-allose biosynthesis	4	2	1
phosphatidylcholine acyl editing	4	2	1
wax esters biosynthesis II	4	1	1
pinosylvin metabolism	4	1	1
long chain fatty acid ester synthesis (engineered)	4	1	1
sporopollenin precursors biosynthesis	18	5	4
polyisoprenoid biosynthesis (E. coli)	5	5	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
5,6-dehydrokavain biosynthesis (engineered)	10	8	2
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
dTDP-3-acetamido-α-D-fucose biosynthesis	5	2	1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis	5	2	1
dTDP-α-D-mycaminose biosynthesis	5	2	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	2	1
octane oxidation	5	2	1
purine nucleotides degradation II (aerobic)	11	11	2
stearate biosynthesis II (bacteria and plants)	6	5	1
fatty acid salvage	6	5	1
stearate biosynthesis IV	6	4	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis	6	2	1
dTDP-D-desosamine biosynthesis	6	2	1
6-gingerol analog biosynthesis (engineered)	6	2	1
stearate biosynthesis I (animals)	6	1	1
dTDP-β-L-digitoxose biosynthesis	7	3	1
dTDP-β-L-olivose biosynthesis	7	3	1
ceramide degradation by α-oxidation	7	2	1
dTDP-β-L-mycarose biosynthesis	7	2	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
dTDP-β-L-megosamine biosynthesis	8	3	1
2-deoxy-D-ribose degradation II	8	3	1
dTDP-β-L-4-epi-vancosamine biosynthesis	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	4	1
dTDP-α-D-forosamine biosynthesis	9	3	1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis	9	3	1
superpathway of enterobacterial common antigen biosynthesis	10	9	1
lipid A-core biosynthesis (E. coli K-12)	10	9	1
lipid A-core biosynthesis (Salmonella)	10	8	1
peptidoglycan recycling II	10	7	1
3-phenylpropanoate degradation	10	4	1
nucleoside and nucleotide degradation (archaea)	10	4	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
O-antigen building blocks biosynthesis (E. coli)	11	11	1
L-methionine salvage cycle III	11	5	1
peptidoglycan recycling I	14	14	1
superpathway of purine nucleotide salvage	14	13	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	2	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	7	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of erythromycin biosynthesis	19	2	1
superpathway of megalomicin A biosynthesis	22	3	1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis	23	3	1
superpathway of Kdo2-lipid A biosynthesis	25	24	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
palmitate biosynthesis III	29	21	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	14	1
oleate β-oxidation	35	32	1