Experiment set4IT076 for Pseudomonas simiae WCS417
LB with Phosphomycin disodium salt 0.001 mg/ml
Group: stressMedia: LB + Phosphomycin disodium salt (0.001 mg/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/1/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1163 B5,B6
Specific Phenotypes
For 3 genes in this experiment
For stress Phosphomycin disodium salt in Pseudomonas simiae WCS417
For stress Phosphomycin disodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycerol and Glycerol-3-phosphate Uptake and Utilization | 2 |
| Alginate metabolism | 1 |
| Glycerolipid and Glycerophospholipid Metabolism in Bacteria | 1 |
| MLST | 1 |
| Mannose Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation I | 2 | 1 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| glycerol degradation I | 3 | 3 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| glycerol and glycerophosphodiester degradation | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 8 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 6 | 1 |