Experiment set4IT071 for Pseudomonas simiae WCS417
LB with Paraquat dichloride 0.002 mg/ml
Group: stressMedia: LB + Paraquat dichloride (0.002 mg/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/1/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1161 A7,A8
Specific Phenotypes
For 6 genes in this experiment
For stress Paraquat dichloride in Pseudomonas simiae WCS417
For stress Paraquat dichloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 2 |
| Glutathione-regulated potassium-efflux system and associated functions | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| choline degradation I | 2 | 2 | 1 |
| β-alanine biosynthesis IV | 2 | 1 | 1 |
| β-alanine biosynthesis I | 2 | 1 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |
| glycine betaine biosynthesis III (plants) | 3 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis I (Wollastonia) | 3 | 1 | 1 |
| choline degradation IV | 4 | 2 | 1 |
| dimethylsulfoniopropanoate biosynthesis II (Spartina) | 4 | 1 | 1 |