Experiment set4IT071 for Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

LB_plus_SM_buffer with Br60_phage 0.000005 MOI

Group: phage
Media: LB_plus_SM_buffer + Br60_phage (5e-06 MOI) + Kanamycin sulfate (0.05 mg/mL), pH=7.2
Culturing: MS1868_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=750rpm
By: Ben Adler on 7/30/18
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 2 genes in this experiment

For phage Br60_phage in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

For phage Br60_phage across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
putrescine biosynthesis I	2	2	1
superpathway of putrescine biosynthesis	4	3	1
superpathway of polyamine biosynthesis I	8	7	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	7	1
superpathway of L-arginine and L-ornithine degradation	13	9	1
superpathway of arginine and polyamine biosynthesis	17	16	1