Experiment set4IT069 for Escherichia coli BW25113

Compare to:

LB with capsaicin 2 mM

200 most detrimental genes:

  gene name fitness t score description  
b1984 asnW +0.9 1.7 tRNA-Asn (NCBI) compare
b0782 moaB +0.8 1.5 molybdopterin biosynthesis protein B (NCBI) compare
b4394 yjjX +0.8 2.9 orf, hypothetical protein (VIMSS) compare
b1429 tehA +0.7 1.8 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1058 yceO +0.7 2.2 hypothetical protein (NCBI) compare
b3134 agaW +0.6 1.9 N-acetylgalactosamine-specific enzyme IIC component of PTS, fragment (pseudogene) (RefSeq) compare
b1150 ymfR +0.6 1.3 e14 prophage; predicted protein (NCBI) compare
b3442 yhhZ +0.6 1.9 hypothetical protein (NCBI) compare
b0858 ybjO +0.6 1.3 predicted inner membrane protein (NCBI) compare
b0866 ybjQ +0.6 1.2 hypothetical protein (NCBI) compare
b1926 fliT +0.6 2.4 predicted chaperone (NCBI) compare
b2377 yfdY +0.6 2.2 predicted inner membrane protein (NCBI) compare
b3027 ygiZ +0.5 1.9 conserved inner membrane protein (NCBI) compare
b1427 rimL +0.5 2.3 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b4171 miaA +0.5 3.3 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4451 ryhB +0.5 0.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4441 glmY +0.5 1.4 sRNA activator of glmS mRNA (RefSeq) compare
b4508 peaD +0.5 1.1 putative sensory transduction regulator (VIMSS) compare
b4373 rimI +0.5 1.9 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b0450 glnK +0.5 1.8 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b1523 yneG +0.5 1.8 hypothetical protein (NCBI) compare
b1827 kdgR +0.5 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1693 aroD +0.5 2.9 3-dehydroquinate dehydratase (NCBI) compare
b2410 yfeH +0.5 2.2 predicted inner membrane protein (NCBI) compare
b1217 chaB +0.5 0.9 cation transport regulator (NCBI) compare
b1306 pspC +0.5 2.0 DNA-binding transcriptional activator (NCBI) compare
b0880 cspD +0.5 0.9 cold shock protein homolog (NCBI) compare
b2765 ygcM +0.5 1.6 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b4630 ykgP +0.5 1.7 no description compare
b2237 inaA +0.5 1.7 hypothetical protein (NCBI) compare
b4406 yaeP +0.5 0.9 hypothetical protein (NCBI) compare
b1819 manZ +0.5 2.3 mannose-specific enzyme IID component of PTS (NCBI) compare
b4234 yjgA +0.4 1.8 hypothetical protein (NCBI) compare
b1104 ycfL +0.4 1.6 hypothetical protein (NCBI) compare
b4546 ypeB +0.4 1.0 hypothetical protein (NCBI) compare
b4413 sokC +0.4 1.3 regulatory antisense RNA (NCBI) compare
b2432 yfeY +0.4 1.4 hypothetical protein (NCBI) compare
b3775 ppiC +0.4 1.7 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b1263 trpD +0.4 1.6 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b4704 arrS +0.4 1.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b2614 grpE +0.4 1.4 heat shock protein (NCBI) compare
b1553 ydfP +0.4 1.2 Qin prophage; conserved protein (NCBI) compare
b1669 ydhT +0.4 1.4 hypothetical protein (NCBI) compare
b2293 yfbT +0.4 0.8 putative phosphatase (VIMSS) compare
b4436 ryeC +0.4 0.6 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b0121 speE +0.4 2.4 spermidine synthase (NCBI) compare
b1946 fliN +0.4 1.0 flagellar motor switch protein (NCBI) compare
b1038 csgF +0.4 1.7 predicted transport protein (NCBI) compare
b1823 cspC +0.4 1.0 stress protein, member of the CspA-family (NCBI) compare
b0862 artQ +0.4 1.8 arginine transporter subunit (NCBI) compare
b4060 yjcB +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b4544 yfbW +0.4 1.8 hypothetical protein (NCBI) compare
b3545 proK +0.4 1.0 tRNA-Pro (NCBI) compare
b1291 sapD +0.4 1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1540 ydfH +0.4 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b4509 ylcG +0.4 1.3 DLP12 prophage; predicted protein (NCBI) compare
b1111 ycfQ +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b2095 gatZ +0.4 2.7 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b3504 yhiS +0.4 1.5 hypothetical protein (RefSeq) compare
b2419 yfeK +0.4 1.2 hypothetical protein (NCBI) compare
b3221 yhcH +0.4 1.6 hypothetical protein (NCBI) compare
b2398 yfeC +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1346 ydaQ +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b0500 ybbD +0.4 1.2 hypothetical protein (RefSeq) compare
b3002 yqhA +0.4 0.9 hypothetical protein (NCBI) compare
b1446 ydcY +0.4 0.9 hypothetical protein (NCBI) compare
b2096 gatY +0.4 1.5 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2499 purM +0.4 1.7 phosphoribosylaminoimidazole synthetase (NCBI) compare
b1781 yeaE +0.4 2.2 predicted oxidoreductase (NCBI) compare
b2061 wzb +0.4 1.2 protein-tyrosine phosphatase (NCBI) compare
b4195 ulaC +0.4 1.6 L-ascorbate-specific enzyme IIA component of PTS (NCBI) compare
b4314 fimA +0.4 2.0 major type 1 subunit fimbrin (pilin) (NCBI) compare
b4592 yccB +0.4 0.4 hypothetical protein (RefSeq) compare
b2951 yggS +0.4 1.3 predicted enzyme (NCBI) compare
b3098 yqjD +0.4 1.1 hypothetical protein (NCBI) compare
b2433 b2433 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4675 yoaJ +0.3 1.8 expressed protein (RefSeq) compare
b0523 purE +0.3 1.5 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4227 ytfQ +0.3 1.9 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b0857 potI +0.3 1.2 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1868 yecE +0.3 1.1 hypothetical protein (NCBI) compare
b4477 dgoA +0.3 1.9 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (NCBI) compare
b0389 yaiA +0.3 1.4 hypothetical protein (NCBI) compare
b3029 ygiN +0.3 1.2 quinol monooxygenase (NCBI) compare
b4503 yafF +0.3 0.8 no description compare
b0502 ylbG +0.3 1.4 no description compare
b1418 cybB +0.3 0.9 cytochrome b(561) (VIMSS) compare
b0822 ybiV +0.3 1.2 predicted hydrolase (NCBI) compare
b3120 yhaB +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2488 hyfH +0.3 1.7 hydrogenase 4, Fe-S subunit (NCBI) compare
b2977 glcG +0.3 1.5 hypothetical protein (NCBI) compare
b4519 icdC +0.3 2.1 no description compare
b2673 nrdH +0.3 0.8 glutaredoxin-like protein (NCBI) compare
b0717 ybgP +0.3 1.7 predicted assembly protein (NCBI) compare
b0550 rusA +0.3 1.2 DLP12 prophage; endonuclease RUS (NCBI) compare
b1573 ydfC +0.3 1.0 Qin prophage; predicted protein (RefSeq) compare
b0746 valZ +0.3 1.1 tRNA-Val (NCBI) compare
b1962 yedJ +0.3 1.6 predicted phosphohydrolase (NCBI) compare
b1379 hslJ +0.3 1.6 heat-inducible protein (NCBI) compare
b3646 yicG +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b0714 nei +0.3 1.4 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b0163 yaeH +0.3 1.6 hypothetical protein (NCBI) compare
b0534 sfmF +0.3 2.2 predicted fimbrial-like adhesin protein (NCBI) compare
b3155 yhbQ +0.3 1.8 hypothetical protein (NCBI) compare
b2145 yeiS +0.3 1.5 predicted inner membrane protein (NCBI) compare
b4567 yjjZ +0.3 1.9 hypothetical protein (NCBI) compare
b4144 yjeI +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b2156 lysP +0.3 2.4 lysine transporter (NCBI) compare
b1188 ycgB +0.3 1.9 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b4374 yjjG +0.3 1.8 nucleotidase (NCBI) compare
b1595 ynfL +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2259 pmrD +0.3 0.8 polymyxin resistance protein B (VIMSS) compare
b1670 ydhU +0.3 1.6 predicted cytochrome (NCBI) compare
b1754 b1754 +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b1633 nth +0.3 1.3 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b2021 hisC +0.3 1.2 histidinol-phosphate aminotransferase (NCBI) compare
b3136 agaS +0.3 1.7 tagatose-6-phosphate ketose/aldose isomerase (NCBI) compare
b2718 hycH +0.3 1.3 protein required for maturation of hydrogenase 3 (NCBI) compare
b1846 yebE +0.3 1.5 hypothetical protein (NCBI) compare
b2768 ygcP +0.3 1.6 predicted anti-terminator regulatory protein (NCBI) compare
b0430 cyoC +0.3 1.2 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b3206 npr +0.3 1.8 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4614 sokA +0.3 1.6 no description compare
b2442 intZ +0.3 1.7 CPZ-55 prophage; predicted integrase (RefSeq) compare
b4001 yjaH +0.3 1.3 hypothetical protein (NCBI) compare
b0238 gpt +0.3 0.9 xanthine phosphoribosyltransferase (NCBI) compare
b4533 ynfO +0.3 1.0 Qin prophage; predicted protein (NCBI) compare
b2248 yfaX +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4655 ythA +0.3 0.9 expressed protein (RefSeq) compare
b1730 ydjO +0.3 1.3 predicted protein (RefSeq) compare
b1575 dicB +0.3 1.4 Qin prophage; cell division inhibition protein (NCBI) compare
b0248 yafX +0.3 1.3 CP4-6 prophage; predicted protein (NCBI) compare
b0236 prfH +0.3 1.4 peptide chain release factor 2 (NCBI) compare
b4158 yjeO +0.3 1.3 conserved inner membrane protein (NCBI) compare
b4193 sgaT +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b2094 gatA +0.3 1.7 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b0120 speD +0.3 2.0 S-adenosylmethionine decarboxylase proenzyme (NCBI) compare
b1728 ydjM +0.3 1.1 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1484 ddpD +0.3 1.4 D-ala-D-ala transporter subunit (NCBI) compare
b4000 hupA +0.3 1.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0836 yliH +0.3 0.8 hypothetical protein (NCBI) compare
b1312 ycjP +0.3 1.5 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4319 fimG +0.3 1.4 minor component of type 1 fimbriae (NCBI) compare
b0158 btuF +0.3 1.8 vitamin B12-transporter protein BtuF (NCBI) compare
b4438 cyaR +0.3 0.8 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1920 fliY +0.3 0.9 cystine transporter subunit (NCBI) compare
b4369 leuP +0.3 1.0 tRNA-Leu (NCBI) compare
b1305 pspB +0.3 0.6 phage shock protein B (NCBI) compare
b1300 puuC +0.3 0.9 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b3483 yhhH +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b4603 rseX +0.3 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1336 ydaH +0.3 1.4 putative pump protein (transport) (VIMSS) compare
b1635 gst +0.3 1.5 glutathionine S-transferase (NCBI) compare
b3868 glnG +0.3 1.5 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0250 ykfB +0.3 1.3 CP4-6 prophage; predicted protein (NCBI) compare
b0207 dkgB +0.3 1.7 2,5-diketo-D-gluconate reductase B (NCBI) compare
b2455 eutE +0.3 0.9 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b3378 yhfU +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b0825 mipB +0.3 1.1 putative transaldolase (VIMSS) compare
b1853 yebK +0.3 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1270 btuR +0.3 0.8 cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI) compare
b0453 ybaY +0.3 1.5 glycoprotein/polysaccharide metabolism (VIMSS) compare
b3806 cyaA +0.3 1.5 adenylate cyclase (NCBI) compare
b0527 ybcI +0.3 1.0 conserved inner membrane protein (NCBI) compare
b2919 ygfG +0.3 1.3 putative enzyme (VIMSS) compare
b2445 yffN +0.3 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b4561 insM +0.3 1.3 transposase (RefSeq) compare
b1729 ydjN +0.3 0.9 predicted transporter (NCBI) compare
b2725 hycA +0.3 1.0 regulator of the transcriptional regulator FhlA (NCBI) compare
b1315 ycjS +0.3 1.6 predicted oxidoreductase, NADH-binding (NCBI) compare
b2846 yqeH +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b4370 leuQ +0.3 0.9 tRNA-Leu (NCBI) compare
b4660 yhiL +0.3 1.0 no description compare
b0560 nohB +0.3 0.7 DLP12 prophage; DNA packaging protein (NCBI) compare
b1012 b1012 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b1949 fliQ +0.2 1.0 flagellar biosynthesis protein Q (NCBI) compare
b0099 mutT +0.2 1.2 nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP (NCBI) compare
b1685 ydiH +0.2 0.5 predicted protein (RefSeq) compare
b1818 manY +0.2 0.8 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0252 yafZ +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b3739 atpI +0.2 1.6 membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS) compare
b1426 ydcH +0.2 0.6 orf, hypothetical protein (VIMSS) compare
b1101 ptsG +0.2 1.9 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b4329 yjiG +0.2 1.2 conserved inner membrane protein (NCBI) compare
b1410 ynbC +0.2 1.7 predicted hydrolase (NCBI) compare
b1476 fdnI +0.2 0.5 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1891 flhC +0.2 1.3 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b0852 rimK +0.2 1.2 ribosomal protein S6 modification protein (NCBI) compare
b2183 rsuA +0.2 1.1 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b1004 wrbA +0.2 1.4 TrpR binding protein WrbA (NCBI) compare
b1303 pspF +0.2 1.0 psp operon transcriptional activator (VIMSS) compare
b2100 yegV +0.2 1.5 predicted kinase (NCBI) compare
b4367 fhuF +0.2 1.8 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b1482 osmC +0.2 0.8 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b0929 ompF +0.2 1.6 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2236 yfaE +0.2 0.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3361 fic +0.2 1.0 stationary-phase protein, cell division (NCBI) compare
b2500 purN +0.2 1.3 phosphoribosylglycinamide formyltransferase (NCBI) compare
b0708 phr +0.2 1.3 deoxyribodipyrimidine photolyase, FAD-binding (NCBI) compare
b4598 yncL +0.2 0.6 hypothetical protein (RefSeq) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress capsaicin across organisms