Experiment set4IT049 for Agrobacterium fabrum C58

R2A with Spectinomycin dihydrochloride pentahydrate 0.002 mM

Group: stress
Media: R2A + Spectinomycin dihydrochloride pentahydrate (0.002 mM)
Culturing: Agro_ML11, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 28 (C), shaken=700 rpm
By: Hans Carlson and Mitchell Thompson on 22-Jun-21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1 D7

Specific Phenotypes

For 5 genes in this experiment

For stress Spectinomycin dihydrochloride pentahydrate in Agrobacterium fabrum C58

For stress Spectinomycin dihydrochloride pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
RNA processing and degradation, bacterial	1
Terminal cytochrome d ubiquinol oxidases	1
Terminal cytochrome oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Arginine and proline metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation I	1	1	1
L-alanine degradation II (to D-lactate)	3	3	1
ethene biosynthesis IV (engineered)	3	1	1
tRNA processing	10	10	2
4-aminobutanoate degradation V	7	2	1
L-glutamate degradation XI (reductive Stickland reaction)	7	1	1
L-glutamate degradation V (via hydroxyglutarate)	10	4	1
methylaspartate cycle	19	12	1