Experiment set4IT046 for Escherichia coli BL21
Mixed culture, Halobacteriovorax-10
Group: baloMedia: M9 (0.1x) + CaCl2, MgSO4 (5mM ), pH=7
Culturing: BL21_ML4, 48-well_plate, Aerobic, at 30C (C), shaken=700 rpm
By: AR on 5/19/21
Media components: 0.4 g/L D-Glucose, 0.2 mM Magnesium sulfate, 0.01 mM Calcium chloride, 1.28 g/L Sodium phosphate dibasic heptahydrate, 0.3 g/L Potassium phosphate monobasic, 0.05 g/L Sodium Chloride, 0.1 g/L Ammonium chloride (final concentrations)
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Ammonia assimilation | 2 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 2 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate biosynthesis I | 2 | 2 | 2 |
| L-glutamate biosynthesis IV | 1 | 1 | 1 |
| L-glutamate biosynthesis III | 1 | 1 | 1 |
| L-glutamine degradation II | 1 | 1 | 1 |
| L-glutamine degradation I | 1 | 1 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 2 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 4 |
| ammonia assimilation cycle I | 2 | 2 | 1 |
| superpathway of ammonia assimilation (plants) | 3 | 2 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 1 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 1 | 1 |
| L-citrulline biosynthesis | 8 | 6 | 1 |
| superpathway of L-citrulline metabolism | 12 | 8 | 1 |