Experiment set4IT045 for Escherichia coli BL21
Mixed culture, Halobacteriovorax-1
Group: baloMedia: M9 (0.1x), pH=7
Culturing: BL21_ML4, flask, Aerobic, at 30C (C), shaken=200 rpm
By: AR on 05/19/2021
Media components: 0.4 g/L D-Glucose, 0.2 mM Magnesium sulfate, 0.01 mM Calcium chloride, 1.28 g/L Sodium phosphate dibasic heptahydrate, 0.3 g/L Potassium phosphate monobasic, 0.05 g/L Sodium Chloride, 0.1 g/L Ammonium chloride (final concentrations)
Specific Phenotypes
For 3 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Arginine Biosynthesis extended | 1 |
| Lysine Biosynthesis DAP Pathway | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lysine biosynthesis
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-ornithine biosynthesis I | 5 | 5 | 1 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 1 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| L-arginine biosynthesis I (via L-ornithine) | 9 | 9 | 1 |
| L-arginine biosynthesis III (via N-acetyl-L-citrulline) | 9 | 8 | 1 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 9 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 16 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 18 | 1 |
| aspartate superpathway | 25 | 24 | 1 |