Experiment set4IT035 for Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

Group: phage
Media: LB_plus_SM_buffer + FelixO1_phage (wt new) (0.075 MOI) + Kanamycin sulfate (0.05 mg/mL), pH=7.2
Culturing: MS1868_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=750rpm
By: Ben Adler on 7/28/18
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 2 genes in this experiment

For phage FelixO1_phage (wt new) in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

For phage FelixO1_phage (wt new) across organisms

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	1
Pyridoxin (Vitamin B6) Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Pyrimidine metabolism
• Geraniol degradation
• Lysine biosynthesis
• Arginine and proline metabolism
• Tryptophan metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Vitamin B6 metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UMP biosynthesis I	6	6	1
UMP biosynthesis II	6	6	1
UMP biosynthesis III	6	5	1
pyridoxal 5'-phosphate biosynthesis I	7	7	1
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	9	1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	12	11	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	1